The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants
Yıl: 2021 Cilt: 27 Sayı: 2 Sayfa Aralığı: 225 - 233 Metin Dili: İngilizce DOI: 10.9775/kvfd.2020.25106 İndeks Tarihi: 03-10-2021
The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants
Öz: Although the clinical and pathological findings are important in the diagnosis of listeriosis, to isolation or to be shown the presence of the bacterium must be required for the definitive diagnosis. This study aims to investigate the availability of imprint cytological (IC) and immunocytochemical (ICC) methods in comparison with histopathological and immunohistochemical (IHC) methods for the rapid diagnosis of encephalitic listeriosis . In the study, the touching and smear preparations taken from the pons and medulla oblongata of 25 ruminants suspected with listeriosis by neurological symptoms were stained with modified giemsa and also with ICC technic for revealing antigens, as a new method. Same tissue sections were stained with Hematoxylin&Eosin and IHC methods too, and examined under light microscope by scoring. In IC examinations, there were intensive neutrophils in 14 cases and few neutrophils in 4 cases, and no neutrophils were observed in 7 cases. In histopathological examinations, 13 of these 14 cases revealed typical microabscesses and listeria positivity in IHC staining. ICC positivity was detected in 12 (92.3%) of the listeria positive 13 cases. A highly positive correlation was observed among cytology (14), ICC (12), histopathology and IHC (13) scores (r2> 0.8; P<0.01). In conclusion, the cytological examination of the pons and medulla oblongata of listeriosis-suspected ruminants revealed that a rapid pre-diagnosis could be made with the presence of intense neutrophils. Also, with ICC staining of cytological preparations, the diagnosis could be performed with 92.3% accuracy. Since ICC is an easy and fast method, it is concluded that it can be used safely especially in field studies, along with cytological examination.
Anahtar Kelime: Ruminantlarda Ensefalitik Listeriozisin Hızlı Tanısı İçin Sitolojik ve İmmunositolojik Yöntemlerin Kullanılabilirliğinin Araştırılması
Öz: Listeriozisin tanısında klinik ve patolojik bulgular önemli ise de, kesin teşhiste etken izolasyonu veya etkenin varlığının gösterilmesi gerekmektedir.Bu çalışmada ruminantlarda ensefalitik listeriozisin hızlı teşhisinde sitolojik ve immunositokimyasal (ICC) yöntemlerin, histopatoloji ve immuno histokimyasal (IHC) yöntemle kıyaslanarak rutinde kullanılabilirliklerinin araştırılması amaçlandı. Çalışmada, sinirsel semptomlarla listeriozistenşüphelenilen 25 adet ruminantın (1 sığır, 1 buzağı, 11 koyun, 9 kuzu, 1 keçi ve 2 oğlak) pons ve medulla oblongatasından alınan sürme ve kazıntıpreparatlar modifiye Giemsa ile ve ayrıca ICC yöntemle boyandı. Yine aynı bölümlerden alınan doku örnekleri Hematoksilen-Eozin ve IHC yöntemleriylede boyandı ve skorlanarak ışık mikroskobunda incelendi. Sitolojik muayenelerde 14 olguda yoğun, 4 olguda ise az sayıda nötrofil görülürken, 7 olgudahiç nötrofil gözlenmedi. Sitolojik olarak listeriozis olduğu değerlendirilen bu 14 vakanın 13’ünde histopatolojik incelemelerde tipik mikroapse veperivaskuler hücre infiltrasyonu ile IHC boyamalarında listeria pozitifl iği bulundu. IHC yöntemi baz alındığında, listeria pozitif 13 vakanın 12’sindeICC pozitifl iği (%92.3) belirlendi. Sitolojide az sayıda nötrofil görülen 4 olgu ile nötrofil görülmeyen 7 olguda ise mikroapseye rastlanmadı, IHC veICC de negatif olarak bulundu. Sitoloji (14), ICC (12) ile histopatoloji ve IHC (13) skorları arasında yüksek oranda (r2>0.8; P<0.01) pozitif korelasyongözlendi. Sonuç olarak listeriozis şüpheli ruminantların pons ve medulla oblongatasının sitolojik muayenesinde, yoğun nötrofil görülmesiyle hızlı öntanı konulabileceği, yine sitolojik preparatların ICC yöntemle boyanarak, %92.3 doğrulukla teşhisin yapılabileceği ortaya konuldu. ICC’nin kolay ve hızlıbir yöntem olması sebebiyle özellikle saha çalışmalarında sitolojik muayene ile birlikte güvenle kullanılabileceği sonucuna varıldı.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ÖZDEMİR Ö, ORTATATLI M, Terzi F, Hatipoglu F, ÇIFTÇI M, ATEŞ M (2021). The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants. , 225 - 233. 10.9775/kvfd.2020.25106 |
| Chicago | ÖZDEMİR Özgür,ORTATATLI MUSTAFA,Terzi Funda,Hatipoglu Fatih,ÇIFTÇI MUSTAFA KEMAL,ATEŞ Mehmet Burak The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants. (2021): 225 - 233. 10.9775/kvfd.2020.25106 |
| MLA | ÖZDEMİR Özgür,ORTATATLI MUSTAFA,Terzi Funda,Hatipoglu Fatih,ÇIFTÇI MUSTAFA KEMAL,ATEŞ Mehmet Burak The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants. , 2021, ss.225 - 233. 10.9775/kvfd.2020.25106 |
| AMA | ÖZDEMİR Ö,ORTATATLI M,Terzi F,Hatipoglu F,ÇIFTÇI M,ATEŞ M The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants. . 2021; 225 - 233. 10.9775/kvfd.2020.25106 |
| Vancouver | ÖZDEMİR Ö,ORTATATLI M,Terzi F,Hatipoglu F,ÇIFTÇI M,ATEŞ M The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants. . 2021; 225 - 233. 10.9775/kvfd.2020.25106 |
| IEEE | ÖZDEMİR Ö,ORTATATLI M,Terzi F,Hatipoglu F,ÇIFTÇI M,ATEŞ M "The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants." , ss.225 - 233, 2021. 10.9775/kvfd.2020.25106 |
| ISNAD | ÖZDEMİR, Özgür vd. "The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants". (2021), 225-233. https://doi.org/10.9775/kvfd.2020.25106 |
| APA | ÖZDEMİR Ö, ORTATATLI M, Terzi F, Hatipoglu F, ÇIFTÇI M, ATEŞ M (2021). The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27(2), 225 - 233. 10.9775/kvfd.2020.25106 |
| Chicago | ÖZDEMİR Özgür,ORTATATLI MUSTAFA,Terzi Funda,Hatipoglu Fatih,ÇIFTÇI MUSTAFA KEMAL,ATEŞ Mehmet Burak The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27, no.2 (2021): 225 - 233. 10.9775/kvfd.2020.25106 |
| MLA | ÖZDEMİR Özgür,ORTATATLI MUSTAFA,Terzi Funda,Hatipoglu Fatih,ÇIFTÇI MUSTAFA KEMAL,ATEŞ Mehmet Burak The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.27, no.2, 2021, ss.225 - 233. 10.9775/kvfd.2020.25106 |
| AMA | ÖZDEMİR Ö,ORTATATLI M,Terzi F,Hatipoglu F,ÇIFTÇI M,ATEŞ M The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(2): 225 - 233. 10.9775/kvfd.2020.25106 |
| Vancouver | ÖZDEMİR Ö,ORTATATLI M,Terzi F,Hatipoglu F,ÇIFTÇI M,ATEŞ M The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(2): 225 - 233. 10.9775/kvfd.2020.25106 |
| IEEE | ÖZDEMİR Ö,ORTATATLI M,Terzi F,Hatipoglu F,ÇIFTÇI M,ATEŞ M "The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27, ss.225 - 233, 2021. 10.9775/kvfd.2020.25106 |
| ISNAD | ÖZDEMİR, Özgür vd. "The Usability of Cytological and Immunocytological Methods forRapid Diagnosis of Encephalitic Listeriosis in Ruminants". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27/2 (2021), 225-233. https://doi.org/10.9775/kvfd.2020.25106 |
