Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep

HOU, Shengzhen; XIE, Dejuan Xie; Zhang, Liping; ZHANG, Huaixia Zhang; Qian, Chen; Jianlei, Jia; ZHANG, Yingying Zhang

doi:10.9775/kvfd.2020.25064

Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep

Jianlei JIA, (College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, 810016, Pr Chına)

Dejuan XIE, (College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, 810016, Pr Chına)

Yingying ZHANG, (College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, 810016, Pr Chına)

Huaixia ZHANG, (College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, 810016, Pr Chına)

Liping ZHANG, (College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, Pr Chına)

Shengzhen HOU, (College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, 810016, Pr Chına)

Qian CHEN (College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, 810016, Pr Chına)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2021 Cilt: 27 Sayı: 3 Sayfa Aralığı: 285 - 289 Metin Dili: İngilizce DOI: 10.9775/kvfd.2020.25064 İndeks Tarihi: 03-10-2021

Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep

Öz:

Bone Morphogenetic Protein Receptor-1B (BMPR-1B) is considered as the primary gene in sheep for follicular development and litter size trait. Ithas been defi ned as the most major candidate gene for genetic markers of sheep reproductive performance. In our study, polymorphisms in theBMPR-1B gene 3’-UTR region were investigated in 363 Tibetan sheep (119 Plateau-type Tibetan sheep, 141 Valley-type Tibetan sheep, and 103Oula-type Tibetan sheep) by DNA sequencing analysis. Two single nucleotide polymorphisms (SNPs) were identifi ed, which were G1339A andA1354G. The frequencies of SNPs were in Hardy-Weinberg equilibrium (Chi-square test, P>0.05). AA and AG genotypes were found in the A1354Gvariant of the 3’UTR region, that AA and A were the preponderant genotype and allele, respectively. The χ2 independence test analyses indicatedthat the A1354G variant of BMPR-1B gene 3’UTR region polymorphisms was signifi cantly correlated with litter size in all-types Tibetan sheep (P<0.05).These results demonstrate that the BMPR-1B gene 3’-UTR region might be a potential candidate gene for marker-assisted selection (MAS).

Anahtar Kelime:

Tibet Koyunlarında BMPR-1B Geni 3’-UTR Bölgesi Polimorfizmi İle Batın Genişliği İlişkisi

Öz:

Kemik Morfogenetik Protein Reseptörü-1B (BMPR-1B), koyunlarda foliküler gelişim ve batın genişliği özelliği için birincil gen olarak kabul edilir. Bu gen, koyunlarda üreme performansının genetik belirteçleri için en önemli aday gen olarak tanımlanmıştır. Çalışmamızda, 363 Tibet koyununda (119 plato tipi Tibet koyunu, 141 vadi tipi Tibet koyunu ve 103 Oula tipi Tibet koyunu) BMPR-1B geninin 3’-UTR bölgesindeki polimorfi zmler DNA dizi analizi ile araştırıldı. G1339A ve A1354G adlı iki single nükleotid polimorfi zm (SNP) saptandı. SNP’lerin frekansları Hardy-Weinberg dengesi içerisindeydi (Ki-kare testi, P>0.05). 3’UTR bölgesinin A1354G varyantında AA ve AG genotipleri saptandı ve AA ve A sırasıyla baskın genotip ve aleldi. χ2 bağımsızlık testi analizleri, BMPR-1B geni 3’UTR bölgesi polimorfi zmlerinden A1354G varyantının, tüm Tibet koyun türlerinde batın genişliği ile yakından ilişkili olduğunu gösterdi (P <0.05). Bu sonuçlar, BMPR-1B geni 3’-UTR bölgesinin, markör destekli seleksiyon (MAS) için potansiyel bir aday gen olabileceğini göstermektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Jianlei J, XIE D, ZHANG Y, ZHANG H, Zhang L, HOU S, Qian C (2021). Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep. , 285 - 289. 10.9775/kvfd.2020.25064
Chicago	Jianlei Jia,XIE Dejuan Xie,ZHANG Yingying Zhang,ZHANG Huaixia Zhang,Zhang Liping,HOU Shengzhen,Qian Chen Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep. (2021): 285 - 289. 10.9775/kvfd.2020.25064
MLA	Jianlei Jia,XIE Dejuan Xie,ZHANG Yingying Zhang,ZHANG Huaixia Zhang,Zhang Liping,HOU Shengzhen,Qian Chen Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep. , 2021, ss.285 - 289. 10.9775/kvfd.2020.25064
AMA	Jianlei J,XIE D,ZHANG Y,ZHANG H,Zhang L,HOU S,Qian C Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep. . 2021; 285 - 289. 10.9775/kvfd.2020.25064
Vancouver	Jianlei J,XIE D,ZHANG Y,ZHANG H,Zhang L,HOU S,Qian C Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep. . 2021; 285 - 289. 10.9775/kvfd.2020.25064
IEEE	Jianlei J,XIE D,ZHANG Y,ZHANG H,Zhang L,HOU S,Qian C "Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep." , ss.285 - 289, 2021. 10.9775/kvfd.2020.25064
ISNAD	Jianlei, Jia vd. "Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep". (2021), 285-289. https://doi.org/10.9775/kvfd.2020.25064

APA	Jianlei J, XIE D, ZHANG Y, ZHANG H, Zhang L, HOU S, Qian C (2021). Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27(3), 285 - 289. 10.9775/kvfd.2020.25064
Chicago	Jianlei Jia,XIE Dejuan Xie,ZHANG Yingying Zhang,ZHANG Huaixia Zhang,Zhang Liping,HOU Shengzhen,Qian Chen Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27, no.3 (2021): 285 - 289. 10.9775/kvfd.2020.25064
MLA	Jianlei Jia,XIE Dejuan Xie,ZHANG Yingying Zhang,ZHANG Huaixia Zhang,Zhang Liping,HOU Shengzhen,Qian Chen Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.27, no.3, 2021, ss.285 - 289. 10.9775/kvfd.2020.25064
AMA	Jianlei J,XIE D,ZHANG Y,ZHANG H,Zhang L,HOU S,Qian C Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(3): 285 - 289. 10.9775/kvfd.2020.25064
Vancouver	Jianlei J,XIE D,ZHANG Y,ZHANG H,Zhang L,HOU S,Qian C Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(3): 285 - 289. 10.9775/kvfd.2020.25064
IEEE	Jianlei J,XIE D,ZHANG Y,ZHANG H,Zhang L,HOU S,Qian C "Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27, ss.285 - 289, 2021. 10.9775/kvfd.2020.25064
ISNAD	Jianlei, Jia vd. "Association of BMPR-1B Gene 3’-UTR Region Polymorphism with Litter Size in Tibetan Sheep". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27/3 (2021), 285-289. https://doi.org/10.9775/kvfd.2020.25064