Yıl: 2021 Cilt: 27 Sayı: 3 Sayfa Aralığı: 339 - 346 Metin Dili: İngilizce DOI: 10.9775/kvfd.2021.25439 İndeks Tarihi: 03-10-2021

Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?

Öz:
Glutamate is the major excitatory neurotransmitter in the central nervous system. Excessive glutamate is known to cause excitotoxicity.Umbelliferone is a coumarin derivative compound and has antioxidant, anti-infl ammatory, and neuroprotective eff ects. Also, umbelliferone can showneuroprotective eff ect by crossing the blood-brain barrier. In our study, it was aimed to investigate the neuroprotective eff ect of umbelliferone onprimary cortical neuron (PCN) culture. Umbelliferone was isolated from the roots of Ferulago cassia dichloromethane sub-extract. The cerebral cortexof newborn Sprague Dawley rats was used to obtain PCNs. To stimulate glutamate excitotoxicity, cells were exposed to 6x10-5M glutamate. Thendiff erent concentrations (10-1000 μM) of umbelliferone were added into the medium and allowed to incubate for 24 and 72 h. MTT assay was used tomeasure cell viability. Total Antioxidant Status (TAS) and Total Oxidant Status (TOS) analyzes were used to evaluate reactive oxygen species. MTT resultsshowed that cell viability was decreased with glutamate application. 25-250 µM umbelliferone had a significant protective eff ect against glutamateexcitotoxicity at 72 h (P<0.05). Consistent with MTT results, TAS analysis results showed 50-250 µM umbelliferone increase the level of antioxidantsin cells, which can help protect neurons against glutamate-induced excitotoxicity. In this study, umbelliferone showed a neuroprotective eff ect inPCN against glutamate excitotoxicity. These results suggest that umbelliferone may be used as therapeutic agent against glutamate excitotoxicity.
Anahtar Kelime:

Umbelliferon, Glutamat Eksitotoksisitesine Karşı Primer Kortikal Nöron Hücrelerini Korur mu?

Öz:
Glutamat merkezi sinir sisteminin ana uyarıcı nörotransmitteridir. Aşırı glutamatın, glutamat reseptörlerinin uzun süreli aktivasyonu nedeniyle eksitotoksisiteye neden olduğu bilinmektedir. Umbelliferon, antioksidan, antiinfl amatuar ve nöroprotektif etkilere sahip kumarin türevi bir bileşiktir. Ayrıca, umbelliferon kan beyin bariyerini geçerek nöronal hücreleri ölümden koruyabilmektedir. Bu çalışmada glutamat eksitotoksisitesine maruz bırakılan primer kortikal nöron (PCN) kültürlerinde umbelliferonun nöroprotektif etkisinin araştırılması amaçlanmaktadır. Umbelliferon, Ferulago cassia diklorometan alt ekstraktının köklerinden izole edildi. PCN’ler yenidoğan Sprague Dawley cinsi sıçanların serebral kortekslerinden elde edilmiştir. Glutamat eksitotoksistesi oluşturmak için hücreler 6x10-5 M glutamata maruz bırakıldı. Daha sonra hücrelere farklı konsantrasyonlarda (10-1000 μM) umbelliferon uygulanarak 24 ve 72 saat inkübasyona bırakıldı. Hücre canlılık oranı MTT yöntemi ile belirlendi. Hücrelerde oluşan reaktif oksijen türleri Total oxidant status (TOS) ve Total antioxidant status (TAS) yöntemleri ile değerlendirildi. MTT analiz sonuçlarına göre, glutamat uygulaması ile hücre canlılığının azaldığı görüldü. 72. saatte 25-250 µM umbelliferonun glutamat eksitotoksisitesine karşı nöronlarda anlamlı düzeyde koruyucu etkiye sahip olduğu tespit edildi (P<0.05). MTT sonuçlarıyla tutarlı olarak TAS analizi sonuçları, 50-250 umbelliferonun hücrelerdeki antioksidan düzeyini artırdığını ve bu da nöronların glutamata bağlı eksitotoksisiteye karşı korunmasına yardımcı olabileceğini gösterdi. Bu çalışmada umbelliferon, PCN hücrelerinde glutamat eksitotoksisitesine karşı nöroprotektif bir etki göstermiştir. Bu sonuçlar ile umbelliferonun glutamat eksitotoksisitesine karşı terapötik bir ajan olarak kullanılabileceği sonucuna varıldı.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA DEMİRKAYA A, GÜNDOGDU G, KARAKAYA S, YILMAZ TASCI S, NALCI K, Hacimuftuoglu A (2021). Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?. , 339 - 346. 10.9775/kvfd.2021.25439
Chicago DEMİRKAYA Alper Kürşat,GÜNDOGDU GÜLSAH,KARAKAYA Songül,YILMAZ TASCI SEYMANUR,NALCI KEMAL ALP,Hacimuftuoglu Ahmet Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?. (2021): 339 - 346. 10.9775/kvfd.2021.25439
MLA DEMİRKAYA Alper Kürşat,GÜNDOGDU GÜLSAH,KARAKAYA Songül,YILMAZ TASCI SEYMANUR,NALCI KEMAL ALP,Hacimuftuoglu Ahmet Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?. , 2021, ss.339 - 346. 10.9775/kvfd.2021.25439
AMA DEMİRKAYA A,GÜNDOGDU G,KARAKAYA S,YILMAZ TASCI S,NALCI K,Hacimuftuoglu A Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?. . 2021; 339 - 346. 10.9775/kvfd.2021.25439
Vancouver DEMİRKAYA A,GÜNDOGDU G,KARAKAYA S,YILMAZ TASCI S,NALCI K,Hacimuftuoglu A Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?. . 2021; 339 - 346. 10.9775/kvfd.2021.25439
IEEE DEMİRKAYA A,GÜNDOGDU G,KARAKAYA S,YILMAZ TASCI S,NALCI K,Hacimuftuoglu A "Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?." , ss.339 - 346, 2021. 10.9775/kvfd.2021.25439
ISNAD DEMİRKAYA, Alper Kürşat vd. "Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?". (2021), 339-346. https://doi.org/10.9775/kvfd.2021.25439
APA DEMİRKAYA A, GÜNDOGDU G, KARAKAYA S, YILMAZ TASCI S, NALCI K, Hacimuftuoglu A (2021). Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27(3), 339 - 346. 10.9775/kvfd.2021.25439
Chicago DEMİRKAYA Alper Kürşat,GÜNDOGDU GÜLSAH,KARAKAYA Songül,YILMAZ TASCI SEYMANUR,NALCI KEMAL ALP,Hacimuftuoglu Ahmet Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27, no.3 (2021): 339 - 346. 10.9775/kvfd.2021.25439
MLA DEMİRKAYA Alper Kürşat,GÜNDOGDU GÜLSAH,KARAKAYA Songül,YILMAZ TASCI SEYMANUR,NALCI KEMAL ALP,Hacimuftuoglu Ahmet Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.27, no.3, 2021, ss.339 - 346. 10.9775/kvfd.2021.25439
AMA DEMİRKAYA A,GÜNDOGDU G,KARAKAYA S,YILMAZ TASCI S,NALCI K,Hacimuftuoglu A Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(3): 339 - 346. 10.9775/kvfd.2021.25439
Vancouver DEMİRKAYA A,GÜNDOGDU G,KARAKAYA S,YILMAZ TASCI S,NALCI K,Hacimuftuoglu A Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(3): 339 - 346. 10.9775/kvfd.2021.25439
IEEE DEMİRKAYA A,GÜNDOGDU G,KARAKAYA S,YILMAZ TASCI S,NALCI K,Hacimuftuoglu A "Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27, ss.339 - 346, 2021. 10.9775/kvfd.2021.25439
ISNAD DEMİRKAYA, Alper Kürşat vd. "Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27/3 (2021), 339-346. https://doi.org/10.9775/kvfd.2021.25439