TY  - JOUR
TI  - Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?
AB  - Glutamate is the major excitatory neurotransmitter in the central nervous system. Excessive glutamate is known to cause excitotoxicity.Umbelliferone is a coumarin derivative compound and has antioxidant, anti-infl ammatory, and neuroprotective eff ects. Also, umbelliferone can showneuroprotective eff ect by crossing the blood-brain barrier. In our study, it was aimed to investigate the neuroprotective eff ect of umbelliferone onprimary cortical neuron (PCN) culture. Umbelliferone was isolated from the roots of Ferulago cassia dichloromethane sub-extract. The cerebral cortexof newborn Sprague Dawley rats was used to obtain PCNs. To stimulate glutamate excitotoxicity, cells were exposed to 6x10-5M glutamate. Thendiff erent concentrations (10-1000 μM) of umbelliferone were added into the medium and allowed to incubate for 24 and 72 h. MTT assay was used tomeasure cell viability. Total Antioxidant Status (TAS) and Total Oxidant Status (TOS) analyzes were used to evaluate reactive oxygen species. MTT resultsshowed that cell viability was decreased with glutamate application. 25-250 µM umbelliferone had a significant protective eff ect against glutamateexcitotoxicity at 72 h (P<0.05). Consistent with MTT results, TAS analysis results showed 50-250 µM umbelliferone increase the level of antioxidantsin cells, which can help protect neurons against glutamate-induced excitotoxicity. In this study, umbelliferone showed a neuroprotective eff ect inPCN against glutamate excitotoxicity. These results suggest that umbelliferone may be used as therapeutic agent against glutamate excitotoxicity.
AU  - DEMİRKAYA, Alper Kürşat
AU  - KARAKAYA, Songül
AU  - NALCI, KEMAL ALP
AU  - Hacimuftuoglu, Ahmet
AU  - GÜNDOGDU, GÜLSAH
AU  - YILMAZ TASCI, SEYMANUR
DO  - 10.9775/kvfd.2021.25439
PY  - 2021
JO  - Kafkas Üniversitesi Veteriner Fakültesi Dergisi
VL  - 27
IS  - 3
SN  - 1300-6045
SP  - 339
EP  - 346
DB  - TRDizin
UR  - http://search/yayin/detay/447860
ER  -