The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows
Yıl: 2021 Cilt: 27 Sayı: 3 Sayfa Aralığı: 389 - 395 Metin Dili: İngilizce DOI: 10.9775/kvfd.2021.25714 İndeks Tarihi: 04-10-2021
The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows
Öz: This study aims to investigate the eff ects of three single nucleotide (SNPs) polymorphisms of the MBL1 gene on subclinical mastitis forHolstein cows. For this study, a total of 151 Holstein cows were selected in their third lactation. The subclinical mastitis conditions weredetermined using the California Mastitis Test (CMT). Genotyping was carried out with the PCR-RFLP method. The results revealed that thewild-type allele frequencies were 0.72 (G allele), 0.40 (G allele), and 0.37 (T allele) for 1252 G>A, 2534 G>A, and 2569 T>C SNPs, respectively.Among the investigated SNPs, only the 1252 G>A SNP was not found in Hardy-Weinberg equilibrium. The eff ects of SNPs on subclinicalmastitis were modeled using multiple logistic regression analysis. The established model can identify cows with subclinical mastitis with aseparation efficiency of 62.3%. It was thought that 1252 G>A and 2534 G>A SNPs may aff ect subclinical mastitis rates.
Anahtar Kelime: Holstein İneklerinde MBL1 Geni Polimorfizminin Subklinik Mastitis Üzerine Etkileri
Öz: Bu çalışma, MBL1 geninde bulunan üç tek nükleotid polimorfizminin (SNP) Holstein ineklerinde subklinik mastitis üzerine etkilerini incelemeyi amaçlamaktadır. Çalışma için üçüncü laktasyonlarında olduğu bildirilen toplam 151 Holstein inek seçilmiştir. Subklinik mastitis kondüsyonları California Mastitis Testi (CMT) kullanılarak belirlenmiştir. Genotipleme PCR-RFLP yöntemi kullanılarak gerçekleştirilmiştir. Sonuç olarak doğal tip allel frekanslarının 1252 G>A, 2534 G>A ve 2569 T>C SNP’leri için sırasıyla 0.72 (G allel), 0.40 (G allel) ve 0.37 (T allel) olduğu belirlenmiştir. İncelenen SNP’ler arasında sadece 1252 G>A SNP’sinin Hardy-Weinberg dengesinde olmadığı belirlenmiştir. SNP’lerin subklinik mastitis üzerindeki etkileri, çok değişkenli lojistik regresyon analizi kullanılarak modellenmiştir. Oluşturulan model, subklinik mastitisli inekleri %62.3 verimlilikle belirleyebilmektedir. 1252 G>A ve 2534 G>A SNP’lerin subklinik mastitis oranları için etkili faktörler olabileceği düşünülmüştür.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | aksel e, AKÇAY A, arslan k, Sohel M, Güngör G, AKYÜZ B (2021). The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows. , 389 - 395. 10.9775/kvfd.2021.25714 |
| Chicago | aksel esma gamze,AKÇAY Aytaç,arslan korhan,Sohel Md Mahmodul Hasan,Güngör Güven,AKYÜZ Bilal The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows. (2021): 389 - 395. 10.9775/kvfd.2021.25714 |
| MLA | aksel esma gamze,AKÇAY Aytaç,arslan korhan,Sohel Md Mahmodul Hasan,Güngör Güven,AKYÜZ Bilal The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows. , 2021, ss.389 - 395. 10.9775/kvfd.2021.25714 |
| AMA | aksel e,AKÇAY A,arslan k,Sohel M,Güngör G,AKYÜZ B The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows. . 2021; 389 - 395. 10.9775/kvfd.2021.25714 |
| Vancouver | aksel e,AKÇAY A,arslan k,Sohel M,Güngör G,AKYÜZ B The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows. . 2021; 389 - 395. 10.9775/kvfd.2021.25714 |
| IEEE | aksel e,AKÇAY A,arslan k,Sohel M,Güngör G,AKYÜZ B "The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows." , ss.389 - 395, 2021. 10.9775/kvfd.2021.25714 |
| ISNAD | aksel, esma gamze vd. "The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows". (2021), 389-395. https://doi.org/10.9775/kvfd.2021.25714 |
| APA | aksel e, AKÇAY A, arslan k, Sohel M, Güngör G, AKYÜZ B (2021). The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27(3), 389 - 395. 10.9775/kvfd.2021.25714 |
| Chicago | aksel esma gamze,AKÇAY Aytaç,arslan korhan,Sohel Md Mahmodul Hasan,Güngör Güven,AKYÜZ Bilal The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27, no.3 (2021): 389 - 395. 10.9775/kvfd.2021.25714 |
| MLA | aksel esma gamze,AKÇAY Aytaç,arslan korhan,Sohel Md Mahmodul Hasan,Güngör Güven,AKYÜZ Bilal The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.27, no.3, 2021, ss.389 - 395. 10.9775/kvfd.2021.25714 |
| AMA | aksel e,AKÇAY A,arslan k,Sohel M,Güngör G,AKYÜZ B The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(3): 389 - 395. 10.9775/kvfd.2021.25714 |
| Vancouver | aksel e,AKÇAY A,arslan k,Sohel M,Güngör G,AKYÜZ B The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(3): 389 - 395. 10.9775/kvfd.2021.25714 |
| IEEE | aksel e,AKÇAY A,arslan k,Sohel M,Güngör G,AKYÜZ B "The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27, ss.389 - 395, 2021. 10.9775/kvfd.2021.25714 |
| ISNAD | aksel, esma gamze vd. "The Eff ects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27/3 (2021), 389-395. https://doi.org/10.9775/kvfd.2021.25714 |
