EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE
Yıl: 2021 Cilt: 31 Sayı: 3 Sayfa Aralığı: 379 - 384 Metin Dili: İngilizce DOI: 10.17567/ataunidfd.933234 İndeks Tarihi: 04-10-2021
EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE
Öz: Aim: The aim of this study was to compare the effect of EDTA with different temperatures and pH values on the penetrabilityof a sealer into dentinal tubules by the confocal laser scanning microscope.Materials and Methods: Forty-four single rooted extracted mandibular premolars were used. The working length wasdetermined and then root canals were prepared with the Reciproc system. Before the final irrigation procedures, specimenswere randomly distributed into 4 groups according to the temperature and pH values of EDTA (n=11): 22°C pH: 7; 22°C pH 12;40°C pH 7; 40°C pH 12. Teeth were obturated using a labeled sealer mixed with 0.1% Rhodamine B and gutta percha as thecore material. Specimens were then sectioned at 2 and 7 mm from the apex. All the sections were examined under confocalmicroscope to calculate the dentinal tubule peneration area. The data were analysed using linear regression analysis and MannWhitney U tests.Results: The linear regression analysis demonstrated that temperature and area variables had the significant effect on sealerpenetration into dentinal tubules (p<0.05) among the variables (temperature, pH and area). 22°C of EDTA solutions has morepenetration area than 40°C of EDTA solutions (p<0.05) at the coronal. There were no statistically significantly differences at theapical. When total penetration areas were evaluated, coronal penetration was statistically significantly higher than apical(p<0.05).Conclusions: Within the limitation of this study, EDTA at room temperature is more effective than 40°C for chelating at thecoronal part of the root canals.
Anahtar Kelime: FARKLI SICAKLIK VE PH DEĞERLERİNDE EDTA KULLANIMININ SEALER PENETRASYONUNA ETKİSİ: LAZER TARAMALI KONFOKAL MİKROSKOP İLE YAPILMIŞ PİLOT ÇALIŞMA
Öz: Amaç: Bu çalışmada son yıkama solüsyonu olarak farklı sıcaklık ve pH değerlerinde EDTA kullanımının sealer penetrasyonuna etkisinin lazer taramalı konfokal mikroskop ile değerlendirilmesi amaçlanmaktadır. Gereç ve Yöntem: Çalışmada 44 adet çekilmiş, tek köklü ve tek kanallı alt premolar diş kullanıldı. Dişlerin çalışma uzunluğu belirlendikten sonra kemomekanik preparasyonları resiprok sistem ile tamamlandı ve son irrigasyon aşamasında EDTA’nın sıcaklık ve pH değerlerine göre dişler 4 gruba ayrıldı (n=11): Grup 1: 22°C pH: 7; Grup 2: 22°C pH 12; Grup 3: 40°C pH 7; Grup 4: 40°C pH 12. Dişler %0.1 Rhodamine B ile işaretlenmiş kanal patı ve guta perka ile dolduruldu. Kökün apikal kısmından 2 ve 7 mm uzaklıktan horizontal kesitler alındı. Örnekler Lazer Taramalı Konfokal Mikroskop ile incelendi ve patların dentin tübüllerine penetrasyon alanı hesaplandı. Verilerin istatistiksel analizinde; linear regresyon analizi ve Mann-Whitney U testi kullanıldı. Bulgular: Linear regresyon analizi ile EDTA’nın sıcaklığının ve kesitin alındığı yerin sealer penetrasyonuna etkisi olduğu gösterildi (p<0.05). 22°C EDTA solüsyonu, 40°C EDTA solüsyonuna göre koronal üçlüde istatistiksel olarak daha fazla penetrasyon alanı sağladı (p<0.05). Apical üçlüde ise EDTA’nın sıcaklığına bağlı olarak herhangi bir istatistiksel farklılık gözlemlenmedi. Total penetrasyon alanları karşılaştırıldığında, koronal penetrasyon alanı apikal penetrasyon alanından istatistiksel olarak daha fazlaydı (p<0.05). Sonuç: Oda ısısındaki EDTA solüsyonu, 40°C’deki EDTA solüsyonuna göre, kök kanalının koronal üçlüsünde daha fazla penetrasyon alanı sağlar.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Yılmaz Z, Aktemur S, Buzoglu HD, Gümüsderelioglu M. The effect of temperature and pH variations on the surface tension of EDTA solutions. J Endod 2011;37:825-27.
- 2. Kuçi A, Alaçam T, Yavaş Ö, Ergul-Ulger Z, Kayaoglu G. Sealer penetration into dentinal tubules in the presence or absence of smear layer: a confocal laser scanning microscopic study. J Endod 2014;40:1627-31.
- 3. Peters OA. Current challenges and concepts in the preparation of root canal systems: a review. J Endod 2004;30:559-67.
- 4. Peters OA, Schönenberger K, Laib A. Effects of four Ni–Ti preparation techniques on root canal geometry assessed by micro computed tomography. Int Endod J 2001;34:221-30.
- 5. de Paz LC. Redefining the persistent infection in root canals: possible role of biofilm communities. J Endod 2007;33:652-62.
- 6. Peters L, Wesselink P, Buijs J, Van Winkelhoff A. Viable bacteria in root dentinal tubules of teeth with apical periodontitis. J Endod 2001;27:76-81.
- 7. Haragushiku GA, Sousa-Neto MD, Silva-Sousa YT, Alfredo E, Silva SC, Silva RG. Adhesion of endodontic sealers to human root dentine submitted to different surface treatments. Photomed Laser Surg 2010;28:405-10.
- 8. Gharib SR, Tordik PA, Imamura GM, Baginski TA, Goodell GG. A confocal laser scanning microscope investigation of the epiphany obturation system. J Endod 2007;33:957-61.
- 9. Orstavik D. Essential endodontology: prevention and treatment of apical periodontitis: John Wiley & Sons; 2020.
- 10. Zehnder M. Root canal irrigants. J Endod 2006;32:389-98.
- 11. Taşman F, Çehreli ZC, Oğan C, Etikan İ. Surface tension of root canal irrigants. J Endod 2000;26:586-7.
- 12. Abou-Rass M, Patonai FJ. The effects of decreasing surface tension on the flow of irrigating solutions in narrow root canals. Oral Surg Oral Med Oral Pathol Oral Radiol 1982;53:524-6.
- 13. Akcay M, Arslan H, Durmus N, Mese M, Capar ID. Dentinal tubule penetration of AH Plus, iRoot SP, MTA fillapex, and guttaflow bioseal root canal sealers after different final irrigation procedures: A confocal microscopic study. Lasers Surg Med 2016;48:70-6.
- 14. Byström A, Sundqvist G. Bacteriologic evaluation of the effect of 0.5 percent sodium hypochlorite in endodontic therapy. Oral Surg Oral Med Oral Pathol 1983;55:307-12.
- 15. Byström A, Sunvqvist G. The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy. Int Endod J 1985;18:35-40.
- 16. Kokkas AB, Boutsioukis AC, Vassiliadis LP, Stavrianos CK. The influence of the smear layer on dentinal tubule penetration depth by three different root canal sealers: an in vitro study. J Endod 2004;30:100-2.
- 17. Violich D, Chandler N. The smear layer in endodontics–a review. Int Endod J 2010;43:2-15.
- 18. Wu L, Mu Y, Deng X, Zhang S, Zhou D. Comparison of the effect of four decalcifying agents combined with 60 C 3% sodium hypochlorite on smear layer removal. J Endod 2012;38:381-4.
- 19. Jardine AP, Da Rosa RA, Santini MF, et al. The effect of final irrigation on the penetrability of an epoxy resin-based sealer into dentinal tubules: a confocal microscopy study. Clin Oral Investig 2016;20:117-23.
- 20. Giardino L, Ambu E, Becce C, Rimondini L, Morra M. Surface tension comparison of four common root canal irrigants and two new irrigants containing antibiotic. J Endod 2006;32:1091-3.
- 21. Cameron JA. The effect of a fluorocarbon surfactant on the surface tension of the endodontic irrigant, sodium hypochlorite: a preliminary report. Aust Dent J 1986;31(5):364-8.
- 22. Calt S, Serper A. Time-dependent effects of EDTA on dentin structures. J Endod 2002;28:17-9.
- 23. Evcil MS, Ersoy İ, Yeter KY, Topcu KM. A comparıson of the accuracy of two dıfferent apex locators usıng dıfferent root canal solutıons. J Dent Fac Ataturk Uni 2012:132-7.
- 24. Baumgartner JC, Mader CL. A scanning electron microscopic evaluation of four root canal irrigation regimens. J Endod 1987;13:147-57.
- 25. Morgan LA, Baumgartner JC. Demineralization of resected root-ends with methylene blue dye. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1997;84:74-8.
- 26. Çiçek E, Keskin Ö. The effect of the temperature changes of EDTA and MTAD on the removal of the smear layer: a scanning electron microscopy study. Scanning 2015;37:193-6.
- 27. Hu X, Ling J, Gao Y. Effects of irrigation solutions on dentin wettability and roughness. J Endod 2010;36:1064-7.
- 28. Zehnder M, Paque F. Disinfection of the root canal system during root canal re‐treatment. Endodontic Topics 2008;19(1):58-73.
- 29. Cury JA, Bragotto C, Valdrighi L. The demineralizing efficiency of EDTA solutions on dentin: I. Influence of pH. Oral Surg Oral Med Oral Pathol 1981;52:446-8.
- 30. Pérez VC, Cárdenas MEM, Planells US. The possible role of pH changes during EDTA demineralization of teeth. Oral Surg Oral Med Oral Pathol 1989;68:220-2.
- 31. Serper A, Çalt S. The demineralizing effects of EDTA at different concentrations and pH. J Endod 2002;28:501-2.
- 32. Eriksson A, Albrektsson T, Grane B, McQueen D. Thermal injury to bone: a vital-microscopic description of heat effects. Int J Oral Surg 1982;11:115-21.
- 33. Tuncer AK, Tuncer S. Effect of different final irrigation solutions on dentinal tubule penetration depth and percentage of root canal sealer. J Endod 2012;38:860-3.
- 34. Patel D, Sherriff M, Ford T, Watson T, Mannocci F. The penetration of RealSeal primer and Tubliseal into root canal dentinal tubules: a confocal microscopic study. Int Endod J 2007;40:67-71.
- 35. Carrigan PJ, Morse DR, Furst ML, Sinai IH. A scanning electron microscopic evaluation of human dentinal tubules according to age and location. J Endod 1984;10:359-63.
- 36. De Deus GA, Gurgel‐Filho ED, Maniglia‐Ferreira C, Coulinho‐Filho T. The influence of filling technique on depth of tubule penetration by root canal sealer: a study using light microscopy and digital image processing. Aust Endod J 2004;30:23-8.
- 37. Kagayama M, Sasano Y, Sato H, Kamakura S, Motegi K, Mizoguchi I. Confocal microscopy of dentinal tubules in human tooth stained with alizarin red. Anat Embryol 1999;199(3):233-8.
- 38. Kok D, Duarte MAH, Da Rosa RA, Wagner MH, Pereira JR, Só MVR. Evaluation of epoxy resin sealer after three root canal filling techniques by confocal laser scanning microscopy. Microsc Res Tech 2012;75:1277-80.
- 39. De‐Deus G, Brandao M, Leal F, et al. Lack of correlation between sealer penetration into dentinal tubules and sealability in nonbonded root fillings. Int Endod J 2012;45:642-51.
| APA | sümbüllü m, ÇOLAK TOPÇU M, Arslan H (2021). EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE. , 379 - 384. 10.17567/ataunidfd.933234 |
| Chicago | sümbüllü meltem,ÇOLAK TOPÇU MELTEM,Arslan Hakan EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE. (2021): 379 - 384. 10.17567/ataunidfd.933234 |
| MLA | sümbüllü meltem,ÇOLAK TOPÇU MELTEM,Arslan Hakan EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE. , 2021, ss.379 - 384. 10.17567/ataunidfd.933234 |
| AMA | sümbüllü m,ÇOLAK TOPÇU M,Arslan H EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE. . 2021; 379 - 384. 10.17567/ataunidfd.933234 |
| Vancouver | sümbüllü m,ÇOLAK TOPÇU M,Arslan H EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE. . 2021; 379 - 384. 10.17567/ataunidfd.933234 |
| IEEE | sümbüllü m,ÇOLAK TOPÇU M,Arslan H "EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE." , ss.379 - 384, 2021. 10.17567/ataunidfd.933234 |
| ISNAD | sümbüllü, meltem vd. "EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE". (2021), 379-384. https://doi.org/10.17567/ataunidfd.933234 |
| APA | sümbüllü m, ÇOLAK TOPÇU M, Arslan H (2021). EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 31(3), 379 - 384. 10.17567/ataunidfd.933234 |
| Chicago | sümbüllü meltem,ÇOLAK TOPÇU MELTEM,Arslan Hakan EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 31, no.3 (2021): 379 - 384. 10.17567/ataunidfd.933234 |
| MLA | sümbüllü meltem,ÇOLAK TOPÇU MELTEM,Arslan Hakan EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, vol.31, no.3, 2021, ss.379 - 384. 10.17567/ataunidfd.933234 |
| AMA | sümbüllü m,ÇOLAK TOPÇU M,Arslan H EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi. 2021; 31(3): 379 - 384. 10.17567/ataunidfd.933234 |
| Vancouver | sümbüllü m,ÇOLAK TOPÇU M,Arslan H EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi. 2021; 31(3): 379 - 384. 10.17567/ataunidfd.933234 |
| IEEE | sümbüllü m,ÇOLAK TOPÇU M,Arslan H "EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE." Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 31, ss.379 - 384, 2021. 10.17567/ataunidfd.933234 |
| ISNAD | sümbüllü, meltem vd. "EFFECT OF DIFFERENT TEMPERATURE AND PH VALUES OF EDTA ON SEALER PENETRATION INTO DENTINAL TUBULES: A PILOT STUDY WITH CONFOCAL LASER SCANNING MICROSCOPE". Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 31/3 (2021), 379-384. https://doi.org/10.17567/ataunidfd.933234 |
