Yıl: 2020 Cilt: 9 Sayı: 2 Sayfa Aralığı: 127 - 133 Metin Dili: İngilizce DOI: 10.46810/tdfd.819741 İndeks Tarihi: 29-07-2022

Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue

Öz:
In this study, the protective effects of boric acid (BA) in the prevention of brain damagecaused by mercury II chloride ( HgCl2) in rats were investigated. In the experiment, 24 adult andWistar albino male rats weighing roundly 200-300 g were used. Group I (Control, n=8): Isotonicsaline (i.p), Group II (HgCl2,n=8):(0.01g/kg)(oral), Group III (HgCl2 + (BA) (n:8): HgCl2(0.01g/kg)+ BA (3.25mg/kg/day i.p.) group were administered. The rats in all groups were sacrificed at theend of the 10th day and their brain tissues were taken. Biochemical parameters including theenzyme activities of SOD, CAT and GSH-Px were measured. The enzyme activity of SOD wasreduced in HgCl2 treated group in comparison to the control group (p<0.001). Activity of theenzyme was increased after BA administration (p˂0.001). CAT enzyme activity decreased in HgCl2and HgCl2+BA administered groups with control (p˂0.001). An increase in enzyme activity in BAgroup with HgCl2 administered group was observed. GSH-Px enzyme activity decreased in HgCl2(p˂0.001) and HgCl2+BA (p˂0.05) groups with control. However, an increase was found in BAadministered group with HgCl2 administered group (p˂0.001). It is thought that antioxidant enzymeactivities such as SOD, CAT and GSH-Px are significantly changed and BA may have a protectiveeffect in the histopathological examination of brain tissue.
Anahtar Kelime:

Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi

Öz:
Bu çalışmada, sıçanlarda civa II klorür'ün meydana getireceği beyin hasarının engellenmesinde borik asit (BA)'in koruyucu etkileri araştırıldı. Deneyde 200-300 gr ağırlığında, 24 adet yetişkin, Wistar albino cinsi erkek rat kullanıldı. Ratlar, Grup I (Kontrol,n:8):İzotonik serum uygulandı (i.p), Grup II (civa II klorür (HgCl2),n:8):Oral yol ile (0.01 g/kg), Grup III (HgCl2+BA,n:8): Oral yol ile HgCl2 (0.01 g/kg/gün) + BA (3.25mg/kg/gün) (i.p) konsantrasyonda uygulaması yapıldı. Tüm gruplardaki ratlar 10. günün sonunda sakrifiye edilerek beyin dokuları alındı. Biyokimyasal parametrelerden SOD, CAT ve GSH-Px antioksidan enzim aktiviteleri ölçüldü. Ayrıca histopatolojik olarak değerlendirildi. SOD enzim aktivitesi değerlendirildiğinde; Hg uygulanan grupta kontrole oranla azaldığı (p˂0.001) görüldü. BA, uygulanan grupta ise aktivitenin HgCl2 grubu ile kıyaslandığında arttığı (p˂0.001) tespit edildi. CAT enzim aktivitesi değerlendirildiğinde; kontrole oranla HgCl2 ve HgCl2+BA uygulanan gruplarda aktivitede azalma (p˂0.001) olduğu görüldü. HgCl2 uygulanan grupla karşılaştırıldığında BA uygulanan grupta enzim aktivitesinde artış belirlendi. GSH-Px enzim aktivitesi değerlendirildiğinde ise; kontrol grubu ile kıyaslandığında HgCl2 (p˂0.001) ve HgCl2+BA (p˂0.05) uygulanan gruplarda aktivitenin azaldığı görüldü. Ayrıca HgCl2 uygulanan grup ile karşılaştırıldığında BA uygulanan grupta artış olduğu görüldü (p˂0.001). SOD, CAT ve GSH-Px gibi antioksidan enzim aktivitelerinin anlamlı olarak değiştiği ve beyin dokusu histopatolojik incelemede BA'nın koruyucu etkisi olabileceği düşünülmektedir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA AKKOYUN H, bengü a, ULUCAN A, Bayramoğlu AKKOYUN M, İZGİ M, ŞAHİN Ö, Ekin S, MELEK S (2020). Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue. , 127 - 133. 10.46810/tdfd.819741
Chicago AKKOYUN Hurrem Turan,bengü aydın şükrü,ULUCAN Aykut,Bayramoğlu AKKOYUN Mahire,İZGİ Mehmet Sait,ŞAHİN Ömer,Ekin Suat,MELEK SULE Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue. (2020): 127 - 133. 10.46810/tdfd.819741
MLA AKKOYUN Hurrem Turan,bengü aydın şükrü,ULUCAN Aykut,Bayramoğlu AKKOYUN Mahire,İZGİ Mehmet Sait,ŞAHİN Ömer,Ekin Suat,MELEK SULE Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue. , 2020, ss.127 - 133. 10.46810/tdfd.819741
AMA AKKOYUN H,bengü a,ULUCAN A,Bayramoğlu AKKOYUN M,İZGİ M,ŞAHİN Ö,Ekin S,MELEK S Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue. . 2020; 127 - 133. 10.46810/tdfd.819741
Vancouver AKKOYUN H,bengü a,ULUCAN A,Bayramoğlu AKKOYUN M,İZGİ M,ŞAHİN Ö,Ekin S,MELEK S Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue. . 2020; 127 - 133. 10.46810/tdfd.819741
IEEE AKKOYUN H,bengü a,ULUCAN A,Bayramoğlu AKKOYUN M,İZGİ M,ŞAHİN Ö,Ekin S,MELEK S "Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue." , ss.127 - 133, 2020. 10.46810/tdfd.819741
ISNAD AKKOYUN, Hurrem Turan vd. "Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue". (2020), 127-133. https://doi.org/10.46810/tdfd.819741
APA AKKOYUN H, bengü a, ULUCAN A, Bayramoğlu AKKOYUN M, İZGİ M, ŞAHİN Ö, Ekin S, MELEK S (2020). Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue. Türk Doğa ve Fen Dergisi, 9(2), 127 - 133. 10.46810/tdfd.819741
Chicago AKKOYUN Hurrem Turan,bengü aydın şükrü,ULUCAN Aykut,Bayramoğlu AKKOYUN Mahire,İZGİ Mehmet Sait,ŞAHİN Ömer,Ekin Suat,MELEK SULE Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue. Türk Doğa ve Fen Dergisi 9, no.2 (2020): 127 - 133. 10.46810/tdfd.819741
MLA AKKOYUN Hurrem Turan,bengü aydın şükrü,ULUCAN Aykut,Bayramoğlu AKKOYUN Mahire,İZGİ Mehmet Sait,ŞAHİN Ömer,Ekin Suat,MELEK SULE Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue. Türk Doğa ve Fen Dergisi, vol.9, no.2, 2020, ss.127 - 133. 10.46810/tdfd.819741
AMA AKKOYUN H,bengü a,ULUCAN A,Bayramoğlu AKKOYUN M,İZGİ M,ŞAHİN Ö,Ekin S,MELEK S Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue. Türk Doğa ve Fen Dergisi. 2020; 9(2): 127 - 133. 10.46810/tdfd.819741
Vancouver AKKOYUN H,bengü a,ULUCAN A,Bayramoğlu AKKOYUN M,İZGİ M,ŞAHİN Ö,Ekin S,MELEK S Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue. Türk Doğa ve Fen Dergisi. 2020; 9(2): 127 - 133. 10.46810/tdfd.819741
IEEE AKKOYUN H,bengü a,ULUCAN A,Bayramoğlu AKKOYUN M,İZGİ M,ŞAHİN Ö,Ekin S,MELEK S "Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue." Türk Doğa ve Fen Dergisi, 9, ss.127 - 133, 2020. 10.46810/tdfd.819741
ISNAD AKKOYUN, Hurrem Turan vd. "Investigation of Protecting Effect of Boric Acid against Mercury II Chloride Toxicity in Rat Brain Tissue". Türk Doğa ve Fen Dergisi 9/2 (2020), 127-133. https://doi.org/10.46810/tdfd.819741