Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri

Selim ÇOMAKLI, (Atatürk Üniversitesi, Veteriner Fakültesi, Patoloji Bölümü, Erzurum, Türkiye)
Esra MANAVOĞLU KİRMAN, (Atatürk Üniversitesi, Veteriner Fakültesi, Patoloji Bölümü, Erzurum, Türkiye)
Mustafa İLERİTÜRK (Atatürk Üniversitesi, Veteriner Fakültesi, Biyokimya Bölümü, Erzurum, Türkiye)
Türk Doğa ve Fen Dergisi
4 2
Yıl: 2020 Cilt: 9 Sayı: 2 Sayfa Aralığı: 83 - 90 Metin Dili: Türkçe DOI: 10.46810/tdfd.809231 İndeks Tarihi: 29-07-2022

Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri

Öz:
Kolistin, gram-negatif bakterilerin neden olduğu enfeksiyonlarda tedavi amacıyla son seçenekolarak kullanılmaktadır. Literatürde kolistinin neden olduğu testis toksisitesi bildirilmiş olmasınarağmen mekanizmasına ilişkin çok az veri bulunmaktadır. Yapılan bu çalışmanın amacı kolistinuygulamasının neden olduğu testis toksisitesinde rutinin (Rut) koruyucu etkilerinin araştırılmasıdır.Bu amaç için 5 adet deneysel grup oluşturuldu; Kontrol, Rut (100 mg kg gün-1), Kolistin (15 mg kggün-1), Kolistin + Rut (50 mg kg gün-1), Kolistin + Rut (100 mg kg gün-1). Uygulama sonrasındaratların testis dokularında malondialdehit (MDA) ve redükte glutatyon (GSH) seviyeleri tespitedildi. Aynı zamanda testis dokularının histopatolojik incelemesi yapıldı ve 8-hidroksi-2'-deoksiguanozin (8-OHdG), nükleer faktörü kappa B (NF-κB) ve kaspaz 3 aktiviteleriimmunohistokimya ile belirlendi. Sonuçlara bakıldığında kolistin uygulamasının rat testisdokularında MDA düzeyini arttırdığı, GSH düzeyini ise azalttığı tespit edilmiştir. Farklı dozlardaRut uygulaması sonrası ise MDA düzeyinde azalma, GSH düzeyinde ise artış gözlendi. Kolistinintestis dokularında neden olduğu hasar sonucu 8-OHdG, NF-κB ve kaspaz 3 immunpozitifliklerininarttığı, özellikle Rut (100 mg kg gün-1) uygulamasından sonra bu proteinlerin aktivite düzeylerininazaldığı belirlendi. Sonuç olarak kolistinin rat testislerinde oksidatif hasar, enflamasyon, DNAhasarı ve apoptoza neden olduğu, Rut uygulamasının kolistin kaynaklı testis hasarında koruyucuetki gösterdiği belirlendi.
Anahtar Kelime: Kolistin Oksidatif stress 8-OHdG Apoptoz Enflamasyon Testis

Protective Effects of Rutin on Oxidative DNA Damage, NF-κB-Mediated Inflammation, and Apoptosis in Colistin-Induced Testicular Damage of Rats

Öz:
Colistin is used as the last-line therapy for treatment of infections caused by gramnegative bacteria. Although testicular toxicity caused by colistin has been reported in the literature, there are very few data regarding its mechanism. The aim of this study was to investigate the protective effects of rutin (Rut) in testicular toxicity caused by colistin treatment. For this purpose, five experimental groups were composed; Control, Rut (100 mg kg day-1 ), Colistin(15 mg kg day1 ), Colistin plus Rut (50 mg kg day-1 ), Colistin + Rut (100 mg kg day-1 ). After the treatment, malondialdehyde (MDA) and glutathione (GSH) levels were determined in testicular tissues of rats. Furthermore, histopathological examination of testicular tissues, 8-hydroxy-2' - deoxyguanosine (8-OHdG), nuclear factor-kappa B (NF-κB) and caspase 3 activities were determined by immunohistochemistry. It was found that colistin treatment increased the MDA level in rat testicular tissues and decreased the GSH level. However, after Rut treatment, a decrease in MDA level and an increase in GSH level were observed. As a result of the toxicity caused by colistin in testicular tissues, it was determined that 8-OHdG, NF-κB and caspase 3 activities increased, and the activity levels of these protein levels decreased after Rut 100 administration. As a conclusion, it was detected that while colistin caused oxidative damage, inflammation, DNA damage, and apoptosis in rat testicular tissues, Rut treatment had a protective effect in colistininduced testicular damage.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ÇOMAKLI S, MANAVOĞLU KİRMAN E, ILERITURK M (2020). Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. , 83 - 90. 10.46810/tdfd.809231
Chicago ÇOMAKLI Selim,MANAVOĞLU KİRMAN ESRA,ILERITURK MUSTAFA Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. (2020): 83 - 90. 10.46810/tdfd.809231
MLA ÇOMAKLI Selim,MANAVOĞLU KİRMAN ESRA,ILERITURK MUSTAFA Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. , 2020, ss.83 - 90. 10.46810/tdfd.809231
AMA ÇOMAKLI S,MANAVOĞLU KİRMAN E,ILERITURK M Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. . 2020; 83 - 90. 10.46810/tdfd.809231
Vancouver ÇOMAKLI S,MANAVOĞLU KİRMAN E,ILERITURK M Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. . 2020; 83 - 90. 10.46810/tdfd.809231
IEEE ÇOMAKLI S,MANAVOĞLU KİRMAN E,ILERITURK M "Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri." , ss.83 - 90, 2020. 10.46810/tdfd.809231
ISNAD ÇOMAKLI, Selim vd. "Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri". (2020), 83-90. https://doi.org/10.46810/tdfd.809231
APA ÇOMAKLI S, MANAVOĞLU KİRMAN E, ILERITURK M (2020). Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. Türk Doğa ve Fen Dergisi, 9(2), 83 - 90. 10.46810/tdfd.809231
Chicago ÇOMAKLI Selim,MANAVOĞLU KİRMAN ESRA,ILERITURK MUSTAFA Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. Türk Doğa ve Fen Dergisi 9, no.2 (2020): 83 - 90. 10.46810/tdfd.809231
MLA ÇOMAKLI Selim,MANAVOĞLU KİRMAN ESRA,ILERITURK MUSTAFA Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. Türk Doğa ve Fen Dergisi, vol.9, no.2, 2020, ss.83 - 90. 10.46810/tdfd.809231
AMA ÇOMAKLI S,MANAVOĞLU KİRMAN E,ILERITURK M Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. Türk Doğa ve Fen Dergisi. 2020; 9(2): 83 - 90. 10.46810/tdfd.809231
Vancouver ÇOMAKLI S,MANAVOĞLU KİRMAN E,ILERITURK M Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri. Türk Doğa ve Fen Dergisi. 2020; 9(2): 83 - 90. 10.46810/tdfd.809231
IEEE ÇOMAKLI S,MANAVOĞLU KİRMAN E,ILERITURK M "Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri." Türk Doğa ve Fen Dergisi, 9, ss.83 - 90, 2020. 10.46810/tdfd.809231
ISNAD ÇOMAKLI, Selim vd. "Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri". Türk Doğa ve Fen Dergisi 9/2 (2020), 83-90. https://doi.org/10.46810/tdfd.809231
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