The histochemical and ultrastructural effects of cadmium on mouse EDL muscle

GEYİKOĞLU, Fatime; Turkez, Hasan; VURALER, Özgen; TEMELLİ, Aysel

The histochemical and ultrastructural effects of cadmium on mouse EDL muscle

Fatime GEYİKOĞLU, (Atatürk Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Erzurum, Türkiye)

Aysel TEMELLİ, (Atatürk Üniversitesi, Eğitim Fakültesi, Biyoloji Bölümü, Erzurum, Türkiye)

Özgen VURALER, (Atatürk Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Erzurum, Türkiye)

Hasan TÜRKEZ (Atatürk Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Erzurum, Türkiye)

Turkish Journal of Zoology

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Yıl: 2005 Cilt: 29 Sayı: 3 Sayfa Aralığı: 283 - 289 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The histochemical and ultrastructural effects of cadmium on mouse EDL muscle

Öz:

Erkek farelerin extensor digitorum longus (EDL) kasları üzerine kadmiyum (Cd)'un histokimyasal ve Ultrastrüktürel etkileri araştırıldı. Farelere Cd'un iki dozu (0,1 ve 1,0 x $10^{-4}$ M) enjekte edildi. Hayvanlar 48 saatin sonunda öldürüldü ve onların EDL kasları çıkarıldı. Kontrol gruplarında süksinik dehidrojenaz (SDH) metodu ile liflerin üç tipi (l, IIA ve IIB) tanımlandı. Liflerin mitokondri ve sarkotübüler içerikleri farklıydı. Cd verilen farelerde EDL kaslarının histokimyasal ve Ultrastrüktürel özellikleri kontrollerdekinden farklı değildi. Bu yüzden, bu uygulama sonuçları ayrıca verilmemektedir. Ancak, kadmiyumun yüksek dozu (1,0 x $10^{-4}$ M) kas liflerinin tüm tiplerinde SDH aktivitesi üzerine artışa neden oldu. Ayrıca, sarkoplazmik retikulumun ve mitokondrilerin Ultrastrüktürel değişiklikleri, enerji rezervlerinde azalma (lipid damlaları, glikojen) (I ve IIA tipleri) ve miyofibriller dejenerasyonlar tesbit edildi. Tip IIB liflerinin sitoplazmasında tane benzeri tortular gözlendi.

Anahtar Kelime: fareler ağır metaller kadmiyum kaslar mitokondri ince yapı sitotoksisite suksinat dehidrogenaz doku kimyası

Konular: Biyoloji

Fare EDL kası üzerine kadmiyumun histokimyasal ve ultrastrüktürel etkileri

Öz:

The histochemical and Ultrastructural effects of cadmium (Cd) on the extensor digitorum longus (EDL) muscles of male mice were investigated. Two doses of Cd (0.1 and 1.0 x $10^{-4}$ M) were injected into the mice. The animals were killed at the end of 48 h and their EDL muscles excised. Three types of fibers (I, IIA and MB) were described by the succinic dehydrogenase (SDH) method in the control groups. The mitochondrial and sarcotubular contents of the fibers were different. The histochemical and Ultrastructural characteristics of the EDL muscles in mice given Cd did not differ from those of the controls. Therefore, the results of this application are not given separately. However, the high dose of Cd (1.0 x $10^{-4}$ M) caused an increase in the SDH activity in all types of muscle fibers. In addition, Ultrastructural alterations of the sarcoplasmic reticulum and mitochondria, a reduction in energetic reserves (lipid droplets, glycogen) (types I and IIA) and myofibrillar degenerations were detected. Grain-like deposits were also observed in the cytoplasm of type IIB fibers.

Anahtar Kelime: cadmium muscles mitochondria ultrastructure cytotoxicity succinate dehydrogenase histochemistry mice heavy metals

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

Al-Nasser, I.A. 2000. Cadmium hepatotoxicity and alterations of the mitochondrial function. J. Toxicol. Clin. Toxicol. 38: 407-413.
Azou, B.L., Dubus, I., Ohayon-Courtes, C, Labouyrie, J.P., Perez, L., Pouvreau, C, Juvet, L. and Cambar, J. 2002. Cadmium induces direct morphological changes in mesangial cell culture. Toxicol. 179 (3): 233-245.
Bancroft, S.D. and Stevens, A. 1982. Theory and Practice of Histological Techniques. Churchill Livingstone, (2nd ed.) Edinburgh, p. 662.
Eddinger, T.J., Moss, R.L. and Cassens, R.G. 1985. Fiber number arid type composition in extensor digitorium longus, soleus, and diaphragm muscles with aging in Fisher 344 rats. J. Histochem. Cytochem. 33 (10): 1033-1041.
Faroon, O.M., Williams, M. and O'Connor, R. 1994. A review of the carcinogenecity of chemicals most frequently found at national priorities list sites. Toxicol. Ind. Health. 10: 203-230.
Freitas, E.M. S., Silva, M.D.P. and Cruz-Höfling, M.A. 2002. Histochemical differences in the responses of predominantly fast-twitch glycolytic muscle and slow-twitch oxidative muscle to veratrine. Toxicon. 40: 1471-1481.
Friedman, P.A. and Gesek, F.A. 1994. Cadmium uptake by kidney distal convolute tubule cells. Toxicol. Appl. Pharmacol. 128: 257-263.
Geyikoğlu, F., Temelli, A. and Özkaral, A. 2002. Morphological adaptation of rat skeletal muscle to a cold environment. Turk. J. Vet. Anim. Sci. 26: 1121-1126.
Ghadially, F.N. 1999. As you like it, Part 2: A critique and historical review of the electron microscopy literature. Ultrastruct. Pathol. 23: 1-17.
Harstad, E.B. and Klaassen, CD. 2002. Gadolinium chloride pretreatment prevents cadmium chloride- induced liver damage in both wild-type and MT-null mice. Toxicol. Appl. Pharmacol. 180: 178-185.
Herzberg, G.R. and Farrell, B. 2003. Fasting-induced, selective loss of fatty acids from muscle triacylglycerols. Nutr. Res. 23 (2): 205-213.
Kim, S.C., Cho, M.K. and Kim, S.G. 2003. Cadmium-induced non- apoptotic cell death mediated by oxidative stress under the condition of sulfhydryl deficiency. Toxicol. Lett. 144 (3): 325- 336.
Kolakowski, J., Baranski, B. and Opalska, B. 1983. Effect of long-term inhalation exposure to cadmium oxide fumes on cardiac muscle ultrastructure in rats. Toxicol. Lett. 19 (3): 273-278.
Lag, M., Helgeland, K., Olsen, I. and Jonsen, J. 1986. Effects of cadmium acetate and sodium selenite on mucociliary functions and adenosine triphosphate content in mouse trachea organ cultures. Toxicol. 39 (3): 323-332.
Novelli, E.L.B., Lopes, A.M., Rodrigues, A.S.E., Novelli Filho, J.L.V.B. and Ribas, B.O. 1999. Superoxide radical and nephrotoxic effect of cadmium exposure. Int. J. Environ. Health Res. 9: 109-116.
Papp, A., Nagymajtenyi, L. and Desi, I. 2003. A study on electrophysiological effects of subchronic cadmium treatment in rats. Environ. Toxicol. Pharmacol. 13 (3): 181-186.
Pawert, M., Triebskorn, R., Graff, S., Berkus, M., Schulz, J. and Köhler, H.R. 1996. Cellular alterations in collembolan midgut cells as a marker of'heavy metal exposure: ultrastructure and intracellular metal distribution. Sci. Total. Environ. 181: 187-200.
Provias, J.P., Ackerley, C.A., Smith, C. and Becker, L.E. 1994. Cadmium encephalopathy: a report with elemental analysis and pathological findings. Acta Neuropathol. 88: 583-586.
Regunathan, A., Cerny, E.A., Villareal, J. and Bhattacharyya, M.H. 2002. Role of fos and src in cadmium-induced decreases in bone mineral content in mice. Toxicol. Appl. Pharm. 185: 25-40.
Satoh, E., Asai, F., Itoh, K., Nishimura, M. and Urakawa, N. 1982. Mechanism of cadmium-induced blockade of neuromuscular transmission. Eur. J. Pharmacol. 77 (4): 251-257.
Segner, M. and Braunbeck, T. 1998. Cellular Response Profile to Chemical Stress. Wiley, Spektrum Akademischer, Verlag, pp. 521-569.
Shen, Y.M. and Sangiah, S. 1995. Na K+, ATPase, glutathione and hydroxyl free radicals in cadmium chloride induced testicular toxicity in mice. Arch. Environ. Contam. Toxicol. 29: 174-179.
Srivastava, D.K. 1982. Comparative effects of copper, cadmium and mercury on tissue glycogen of the catfish, Heteropneustes fossilis (Bloch). Toxicol. Lett. 11 (1- 2): 135-139.
Topashka-Ancheva, M.t Metcheva, R. and Teodorova, S. 2003. Bioaccumulation and damaging action of polymetal industrial dust on laboratory mice Mus musculus alba II. disturbances. Environ. Res. 92 (2): 152-160.
Torre, F.L., Salibian, A. and Ferrari, L. 2000. Biomarkers assessment in juvenile Cyprinus carpio exposed to waterborne cadmium. Environ. Pollut. 109: 277-282.
Xu, J., Maki, D. and Stapleton, S.R. 2003. Mediation of cadmium-induced oxidative damage and glucose-6-phosphate dehydrogenase expression through glutathione depletion. J. Biochem. Mol. Toxicol. 17 (2): 67-75.
Yamano, T., Shimizu, M. and Noda, T. 1998. Comparative effects of repeated administration of cadmium on kidney, spleen, thymus, and bone marrow in 2, 4 and 8 month old male Wistar rats. Toxicol. Sci. 46: 393-402.

APA	GEYİKOĞLU F, TEMELLİ A, VURALER Ö, Turkez H (2005). The histochemical and ultrastructural effects of cadmium on mouse EDL muscle. , 283 - 289.
Chicago	GEYİKOĞLU Fatime,TEMELLİ Aysel,VURALER Özgen,Turkez Hasan The histochemical and ultrastructural effects of cadmium on mouse EDL muscle. (2005): 283 - 289.
MLA	GEYİKOĞLU Fatime,TEMELLİ Aysel,VURALER Özgen,Turkez Hasan The histochemical and ultrastructural effects of cadmium on mouse EDL muscle. , 2005, ss.283 - 289.
AMA	GEYİKOĞLU F,TEMELLİ A,VURALER Ö,Turkez H The histochemical and ultrastructural effects of cadmium on mouse EDL muscle. . 2005; 283 - 289.
Vancouver	GEYİKOĞLU F,TEMELLİ A,VURALER Ö,Turkez H The histochemical and ultrastructural effects of cadmium on mouse EDL muscle. . 2005; 283 - 289.
IEEE	GEYİKOĞLU F,TEMELLİ A,VURALER Ö,Turkez H "The histochemical and ultrastructural effects of cadmium on mouse EDL muscle." , ss.283 - 289, 2005.
ISNAD	GEYİKOĞLU, Fatime vd. "The histochemical and ultrastructural effects of cadmium on mouse EDL muscle". (2005), 283-289.

APA	GEYİKOĞLU F, TEMELLİ A, VURALER Ö, Turkez H (2005). The histochemical and ultrastructural effects of cadmium on mouse EDL muscle. Turkish Journal of Zoology, 29(3), 283 - 289.
Chicago	GEYİKOĞLU Fatime,TEMELLİ Aysel,VURALER Özgen,Turkez Hasan The histochemical and ultrastructural effects of cadmium on mouse EDL muscle. Turkish Journal of Zoology 29, no.3 (2005): 283 - 289.
MLA	GEYİKOĞLU Fatime,TEMELLİ Aysel,VURALER Özgen,Turkez Hasan The histochemical and ultrastructural effects of cadmium on mouse EDL muscle. Turkish Journal of Zoology, vol.29, no.3, 2005, ss.283 - 289.
AMA	GEYİKOĞLU F,TEMELLİ A,VURALER Ö,Turkez H The histochemical and ultrastructural effects of cadmium on mouse EDL muscle. Turkish Journal of Zoology. 2005; 29(3): 283 - 289.
Vancouver	GEYİKOĞLU F,TEMELLİ A,VURALER Ö,Turkez H The histochemical and ultrastructural effects of cadmium on mouse EDL muscle. Turkish Journal of Zoology. 2005; 29(3): 283 - 289.
IEEE	GEYİKOĞLU F,TEMELLİ A,VURALER Ö,Turkez H "The histochemical and ultrastructural effects of cadmium on mouse EDL muscle." Turkish Journal of Zoology, 29, ss.283 - 289, 2005.
ISNAD	GEYİKOĞLU, Fatime vd. "The histochemical and ultrastructural effects of cadmium on mouse EDL muscle". Turkish Journal of Zoology 29/3 (2005), 283-289.