TY  - JOUR
TI  - High-performance Liquid Chromatography Analysis of Nebivolol and Amlodipine and Some Related Compounds in Synthetic Mixture
AB  - Objective: This study aimed to develop and validate a method usinga high-performance liquid chromatography (HPLC) to perform aquantitative analysis of nebivolol (NEB) and amlodipine (AML)along with some related substances in the synthetic mixture.Methods: The separation in the described chromatographic systemwas accomplished using a mobile phase consisting of a mixture ofacetate buffer (pH: 4.5) and acetonitrile and a HPLC C18 column(150 mm x 4.6 mm, 2.6 μm) with gradient elution on a consistentflow rate of 1.3 mL/min. Photodiode array detection was carriedout at a wavelength of 265 nm. According to The InternationalConference on Harmonisation guidelines, the drug was exposed tovarious stress conditions; including photolysis, oxidation, thermaldegradation and hydrolysis under acidic, basic and neutral mediums.Results: Ranges of detection and quantitation limits weredetermined to be 0.2-10.0 μg.mL-1 and 0.25-10.0 μg.mL-1 forNEB and AML, respectively. The relative standard deviation valueswithin and between days precision were determined to be <2%. Forall substances, the average recovery values were determined withinthe range of 98.00%-101.50%.Conclusion: We conclude that this developed analytical procedureapplies to the quality control of drug formulations.
AU  - Çetin Aluç, Çiğdem
AU  - Onal, Armagan
AU  - Önal, Cem
AU  - Aluc, Kadir
DO  - 10.14235/bas.galenos.2020.4372
PY  - 2021
JO  - Bezmiâlem Science
VL  - 9
IS  - 3
SN  - 2148-2373
SP  - 264
EP  - 270
DB  - TRDizin
UR  - http://search/yayin/detay/450489
ER  -