TY  - JOUR
TI  - Investigation of Compatibility Between Serological Tests Used in Laboratory Diagnosis of Brucellosis
AB  - Aim: In the diagnosis of brucellosis, the production of microorganisms in blood orbone marrow culture is considered the gold standard. But it is not always possible toproduce the microorganism. For this reason, serological tests are used to diagnosebrucellosis. Rose Bengal test (RB), Standard Tube Agglutination test (STA),Coombs Test (CT) and 2-Mercaptoethanol (2-ME) tests are common methods.Immunocapture-agglutination test (ICA) and Brucella Coombs Gel test (BCGT) aretests that can detect blocking antibodies along with total antibodies. The aim of ourstudy was to determine whether there is compatibility between the tests used indiagnosis and the ICA and BCGT tests.Material and Methods: Serum samples were taken from patients with a preliminarydiagnosis of brucellosis. RBT testing was performed primarily on all samples. FortyRBT positive and 40 RBT negative sera were included in the study. All serum sampleswere studied by STA, CT, 2-ME, ICA and BCGT methods. The compatibility betweenthe tests were determined by using the kappa (κ) coefficient with the Cohen kappaanalysis method.Results: 28 of 40 patients with RBT positive were detected as positive with STA and2-ME and 30 of them were positive with CT. BCGT and ICA test results were foundpositive in all RBT positive samples. All tests results were found to be negative in 40RBT negative samples. Cohen Kappa analysis found that compliance between RBTand BCGT and ICA (Kappa 1.0 <0.001) was excellent. Compliance between STA,BCGT and ICA tests was found to be good (Kappa 0.7 p< 0.001). CT testing showeda very good level of compatibility between ICA and BCGT (Kappa 0.8 p<0.001).There is a very good compatibility between the 2ME test, ICA and BCGT tests (kappa0.7 0.001), Compliance between BCGT and ICA was also found to be very good(Kappa 1.0, p<0.001).Conclusion: According to these results, with the use of ICA and BCGT tests, both thenumber of tests will be reduced and the time to receive results will be reduced. Butfurther studies are needed to determine the sensitivity and specificity of these testsbased on culture results. But after that, we believe it can be used as a diagnostic test.
AU  - DUMAN, YUCEL
AU  - AY, SELMA
AU  - Güzel, Bensu
DO  - 10.30565/medalanya.880738
PY  - 2021
JO  - Acta Medica Alanya
VL  - 5
IS  - 2
SN  - 2587-0319
SP  - 171
EP  - 174
DB  - TRDizin
UR  - http://search/yayin/detay/450965
ER  -