Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis

KOSE, DUYGU; Halici, Zekai; GÜRBÜZ, Muhammed; MAMAN, ADEM; KÖSE, AHMET; Yayla, Muhammed

doi:10.30565/medalanya.939161

Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis

Duygu KÖSE, (Atatürk Üniversitesi, Tıp Fakültesi, Farmakoloji Anabilim Dalı, Erzurum, Türkiye)

Ahmet KÖSE, (Atatürk Üniversitesi, Tıp Fakültesi, Farmakoloji Anabilim Dalı, Erzurum, Türkiye)

Zekai HALICI, (Atatürk Üniversitesi, Tıp Fakültesi, Farmakoloji Anabilim Dalı, Erzurum, Türkiye)

Muhammet Ali GÜRBÜZ, (Atatürk Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Erzurum, Türkiye)

Adem MAMAN, (Atatürk Üniversitesi, Tıp Fakültesi, Nükleer Tıp Anabilim Dalı, Erzurum, Türkiye)

Muhammed YAYLA (Kafkas Üniversitesi, Tıp Fakültesi, Farmakoloji Anabilim Dalı, Kars, Türkiye)

Acta Medica Alanya

5 1

Yıl: 2021 Cilt: 5 Sayı: 2 Sayfa Aralığı: 164 - 170 Metin Dili: İngilizce DOI: 10.30565/medalanya.939161 İndeks Tarihi: 29-07-2022

Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis

Öz:

Aim: Melatonin promoted osteoblast differentiation and causes an increase in levelsof markers of bone differentiation and proliferation. Ramelteon (RAMEL) activatesmelatonin receptors and binds to these receptors as non-selective. In this study, weinvestigated the preventive effects of the melatonin agonist RAMEL on osteoporosisby radiological, histological, and molecularly.Methods: Groups 1: Control, Group 2: Osteoporosis: Overectomized group (OP),Group 3: OP + ramelteon 2 mg/kg, Group 4: OP + ramelteon 4 mg/kg. 24 animalsunderwent bilateral ovariectomy. RAMEL was administered orally once a day in theprophylactic treatment mode for 8 weeks, 6 weeks after ovariectomy.Results: Fourteen weeks after ovariectomy, there was a significant reduction infemoral bone mineral density (BMD) (g/cm2) in the OP group compared to the controlgroup. Compared to the OP group, RAMEL treatment significantly increased the BMDlevel (p<0.05). Bone matrix protein 2 (BMP2) and runt-related transcription factor 2(RUNX2) mRNA levels were significantly lower in the OP group than in the controlgroup (p<0.05). RUNX2 and BMP2 mRNA levels were significantly higher in theRAMEL treatment groups than in the OP group (p<0.05).Conclusion: To take advantage of the peripheral effects of melatonin, RAMEL, aperipheral melatonin agonist, can be used to prevent osteoporosis.

Anahtar Kelime: melatonin; rameltone; osteoporosis; bone;

Uykusuzluğu tedavi etmek için kullanılan Ramelteon, osteoporoz oluşumunu azaltabilir

Öz:

Amaç: Melatonin osteoblast farklılaşmasını teşvik eder ve kemik farklılaşması ve proliferasyon belirteçlerinin düzeylerinde artışa neden olur. Ramelteon (RAMEL) melatonin reseptörlerini aktive eder ve bu reseptörlere seçici olmayan şekilde bağlanır. Bu çalışmada melatonin agonisti RAMEL' in osteoporoz üzerindeki önleyici etkileri radyolojik, histolojik ve moleküler olarak araştırıldı. Yöntem: Grup 1: Kontrol, Grup 2: Osteoporoz: Over eksizyonu yapılan grup (OP), Grup 3: OP + ramelteon 2 mg/kg, Grup 4: OP + ramelteon 4 mg/kg. 24 hayvana, bilateral ovariektomi uygulandı. RAMEL, ovariektomiden 6 hafta sonra, 8 hafta boyunca profilaktik tedavi modunda günde bir kez oral yoldan uygulandı. Bulgular: Ovariektomiden on dört hafta sonra, kontrol grubuna kıyasla OP grubunda femoral kemik mineral yoğunluğunda (g/cm2) anlamlı bir azalma oldu. OP grubu ile karşılaştırıldığında, RAMEL tedavisi BMD seviyesini önemli ölçüde artırdı (p<0.05). Bone matrix protein 2 (BMP2) ve runt-related transcription factor 2 (RUNX2) mRNA seviyeleri OP grubunda kontrol grubuna göre anlamlı derecede düşüktü (p<0.05). RUNX2 ve BMP2 mRNA seviyeleri, RAMEL tedavi gruplarında OP grubuna göre anlamlı olarak daha yüksekti (p<0.05). Sonuç: Melatoninin periferik etkilerinden yararlanmak için, bir periferik melatonin agonisti olan RAMEL, osteoporozu önlemek için kullanılabilir

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KOSE D, KÖSE A, Halici Z, GÜRBÜZ M, MAMAN A, Yayla M (2021). Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis. , 164 - 170. 10.30565/medalanya.939161
Chicago	KOSE DUYGU,KÖSE AHMET,Halici Zekai,GÜRBÜZ Muhammed,MAMAN ADEM,Yayla Muhammed Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis. (2021): 164 - 170. 10.30565/medalanya.939161
MLA	KOSE DUYGU,KÖSE AHMET,Halici Zekai,GÜRBÜZ Muhammed,MAMAN ADEM,Yayla Muhammed Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis. , 2021, ss.164 - 170. 10.30565/medalanya.939161
AMA	KOSE D,KÖSE A,Halici Z,GÜRBÜZ M,MAMAN A,Yayla M Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis. . 2021; 164 - 170. 10.30565/medalanya.939161
Vancouver	KOSE D,KÖSE A,Halici Z,GÜRBÜZ M,MAMAN A,Yayla M Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis. . 2021; 164 - 170. 10.30565/medalanya.939161
IEEE	KOSE D,KÖSE A,Halici Z,GÜRBÜZ M,MAMAN A,Yayla M "Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis." , ss.164 - 170, 2021. 10.30565/medalanya.939161
ISNAD	KOSE, DUYGU vd. "Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis". (2021), 164-170. https://doi.org/10.30565/medalanya.939161

APA	KOSE D, KÖSE A, Halici Z, GÜRBÜZ M, MAMAN A, Yayla M (2021). Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis. Acta Medica Alanya, 5(2), 164 - 170. 10.30565/medalanya.939161
Chicago	KOSE DUYGU,KÖSE AHMET,Halici Zekai,GÜRBÜZ Muhammed,MAMAN ADEM,Yayla Muhammed Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis. Acta Medica Alanya 5, no.2 (2021): 164 - 170. 10.30565/medalanya.939161
MLA	KOSE DUYGU,KÖSE AHMET,Halici Zekai,GÜRBÜZ Muhammed,MAMAN ADEM,Yayla Muhammed Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis. Acta Medica Alanya, vol.5, no.2, 2021, ss.164 - 170. 10.30565/medalanya.939161
AMA	KOSE D,KÖSE A,Halici Z,GÜRBÜZ M,MAMAN A,Yayla M Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis. Acta Medica Alanya. 2021; 5(2): 164 - 170. 10.30565/medalanya.939161
Vancouver	KOSE D,KÖSE A,Halici Z,GÜRBÜZ M,MAMAN A,Yayla M Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis. Acta Medica Alanya. 2021; 5(2): 164 - 170. 10.30565/medalanya.939161
IEEE	KOSE D,KÖSE A,Halici Z,GÜRBÜZ M,MAMAN A,Yayla M "Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis." Acta Medica Alanya, 5, ss.164 - 170, 2021. 10.30565/medalanya.939161
ISNAD	KOSE, DUYGU vd. "Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis". Acta Medica Alanya 5/2 (2021), 164-170. https://doi.org/10.30565/medalanya.939161