In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells

ONUR, MEHMET; ARACAGOK, YUSUF DORUK; GOKER, ALI HAKAN; Cihangir, Nilufer; AKBAY, ESİN

doi:10.15671/hjbc.680564

In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells

ESİN ÇETİN, (Hacettepe Üniversitesi, Biyoloji Bölümü, Ankara, Türkiye)

Y. Doruk ARACAGÖK, (Hacettepe Üniversitesi, Biyoloji Bölümü, Ankara, Türkiye)

Hakan GÖKER, (Ankara Üniversitesi, Merkezi Enstrümantal Analiz II Laboratuvarı, Ankara, Türkiye)

Mehmet Ali ONUR, (Hacettepe Üniversitesi, Biyoloji Bölümü, Ankara, Türkiye)

Nilüfer CİHANGİR (Hacettepe Üniversitesi, Biyoloji Bölümü, Ankara, Türkiye)

Hacettepe Journal of Biology and Chemistry

4 0

Yıl: 2021 Cilt: 49 Sayı: 1 Sayfa Aralığı: 25 - 36 Metin Dili: İngilizce DOI: 10.15671/hjbc.680564 İndeks Tarihi: 12-10-2021

In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells

Öz:

The aim of this in vitro study was assessment of the cytotoxic level of O-desmethylnaproxen. A mouse connective tissue fibroblast cell line, L929 was exposed to naproxen and O-desmethylnaproxen in different concentrations for 24- and 48- hours. Cell viability was tested by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphnyltetrazolium bromide (MTT), while apoptosis was determined by acridine orange/propidium iodide (AO/PI) double staining method. The control group was free from any agent (Dulbecco’s Modified Eagle’s Medium (DMEM) only) and accepted as 100% cell viability. The IC50 results indicated that the inhibition of 50% cell viability was resulted by the highest concentration of O-desmethylnaproxen (0.7 μg/mL) while none of naproxen concentration was caused 50% cell death. Consequently, to avoid the unacceptable side effects of naproxen metabolites that evacuated by urine; further studies should be conducted to determine the accumulation of naproxen metabolites.

Anahtar Kelime:

Fare Bağ Dokusu Fibroblast Hücreleri Üzerinde Naproksen metaboliti O-Desmetilnaproksenin In Vitro Değerlendirilmesi

Öz:

Bu in vitro çalışmanın amacı, O-desmetilnaproksen’nin sitotoksik seviyesinin belirlenmesidir. Bu amaçla, L929 hücre hattı,fare bağ doku fibroblast hücreleri, 24- ve 48- saat boyunca farklı konsantrasyonlarda naproksen ve O-desmetilnaproksenemaruz bırakılmıştır. Hücre canlılığı MTT ile test edilirken, apoptoz AO/PI çift boyama yöntemi ile belirlenmiştir. Kontrol grubuherhangi bir ajan içermeyen (sadece DMEM) ve % 100 hücre canlılığı olan grup olarak kabul edilmiştir. IC50 sonuçları, % 50hücre ölümüne neden olan grubun en yüksek konsantrasyona sahip 0-desmetilnaproksen’de (0.7 ug / mL) ortaya çıktığıancak naproksen konsantrasyonlarının, % 50 hücre ölümüne neden olmadığı tespit edilmiştir. Sonuç olarak, idrarla vücuttanuzaklaştırılan naproksen metabolitlerinin, dikkate alınmayan yan etkilerinden kaçınmak için; naproksen metabolitlerininbirikimini belirlemek amacı ile daha ileri çalışmalar yapılmalıdır.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	AKBAY E, ARACAGOK Y, GOKER A, ONUR M, Cihangir N (2021). In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells. , 25 - 36. 10.15671/hjbc.680564
Chicago	AKBAY ESİN,ARACAGOK YUSUF DORUK,GOKER ALI HAKAN,ONUR MEHMET,Cihangir Nilufer In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells. (2021): 25 - 36. 10.15671/hjbc.680564
MLA	AKBAY ESİN,ARACAGOK YUSUF DORUK,GOKER ALI HAKAN,ONUR MEHMET,Cihangir Nilufer In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells. , 2021, ss.25 - 36. 10.15671/hjbc.680564
AMA	AKBAY E,ARACAGOK Y,GOKER A,ONUR M,Cihangir N In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells. . 2021; 25 - 36. 10.15671/hjbc.680564
Vancouver	AKBAY E,ARACAGOK Y,GOKER A,ONUR M,Cihangir N In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells. . 2021; 25 - 36. 10.15671/hjbc.680564
IEEE	AKBAY E,ARACAGOK Y,GOKER A,ONUR M,Cihangir N "In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells." , ss.25 - 36, 2021. 10.15671/hjbc.680564
ISNAD	AKBAY, ESİN vd. "In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells". (2021), 25-36. https://doi.org/10.15671/hjbc.680564

APA	AKBAY E, ARACAGOK Y, GOKER A, ONUR M, Cihangir N (2021). In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells. Hacettepe Journal of Biology and Chemistry, 49(1), 25 - 36. 10.15671/hjbc.680564
Chicago	AKBAY ESİN,ARACAGOK YUSUF DORUK,GOKER ALI HAKAN,ONUR MEHMET,Cihangir Nilufer In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells. Hacettepe Journal of Biology and Chemistry 49, no.1 (2021): 25 - 36. 10.15671/hjbc.680564
MLA	AKBAY ESİN,ARACAGOK YUSUF DORUK,GOKER ALI HAKAN,ONUR MEHMET,Cihangir Nilufer In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells. Hacettepe Journal of Biology and Chemistry, vol.49, no.1, 2021, ss.25 - 36. 10.15671/hjbc.680564
AMA	AKBAY E,ARACAGOK Y,GOKER A,ONUR M,Cihangir N In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells. Hacettepe Journal of Biology and Chemistry. 2021; 49(1): 25 - 36. 10.15671/hjbc.680564
Vancouver	AKBAY E,ARACAGOK Y,GOKER A,ONUR M,Cihangir N In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells. Hacettepe Journal of Biology and Chemistry. 2021; 49(1): 25 - 36. 10.15671/hjbc.680564
IEEE	AKBAY E,ARACAGOK Y,GOKER A,ONUR M,Cihangir N "In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells." Hacettepe Journal of Biology and Chemistry, 49, ss.25 - 36, 2021. 10.15671/hjbc.680564
ISNAD	AKBAY, ESİN vd. "In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A MouseConnective Tissue Fibroblast Cells". Hacettepe Journal of Biology and Chemistry 49/1 (2021), 25-36. https://doi.org/10.15671/hjbc.680564