Yıl: 2005 Cilt: 10 Sayı: 1 Sayfa Aralığı: 23 - 28 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Callus induction in some hypericum species growing in Turkey

Öz:
Species of the genus Hypericum have attracted scientific interest due to their variety of phytomedicinals and in vitro propagation has been reported as very useful tool in their multiplication and in studying their economically important secondary metabolites. The purpose of this study was to develop suitable media for callus induction in H. linarioides, H. lydum, H. origanifolium, H. venustum and H. scabrum. The seeds were surface sterilised and transferred to Murashige & Skoog basal medium supplemented with BA (benzyladenine) and 2,4-D (2,4-dichlorophenoxyacetic acid) at different rates. Culturing seeds in BA and 2,4-D containing media resulted in green and friable callus induction. According to the results of ANOVA, the media supplemented with B A and 2,4-D in tried doses had a significant effect on callus induction and callus fresh weight depending on species.
Anahtar Kelime: ANOVA Method callus induction Hypericum medicinal plants tissue culture

Konular: Ziraat Mühendisliği
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Anonymous (2005) http://www.ens-lyon.fr/DSVT/AGREG_SVT/ Ressources/Collections/BV/Echbocl/dicots.html.
  • Ayan A K, Çırak C, Kevseroğlu K, Sökmen, A (2005). The effects of explant types and culture constituents on plant regeneration and hypericin content in Hypericum perforatum L. Turkish J Agric, Forest 29:197-204.
  • Azırak S Erdoğrul ÖT (2003). Antibacterial activities of Hypericum scabrum. ISOPS-7, proceedings and abstracts, June 23-26, Ankara, Turkey.
  • Cardoso MA, OlivieraDE (1996). Tissue culture of Hypericum brasiliense Choosy: shoot multiplication and callus induction. Cell fiss.and Org. Cult. 44:91- 94.
  • Davis PH (1988). Flora of Turkey and the East Aegean Islands. Edinburgh, Edinburgh University Press.
  • Holz J, Ostrowiski E (1987). St. John's wort HPLC analysis of main components and their variability in the populations. Deuts. Apothekertz 127:1227- 1230.
  • Kartnig T, Brantner A (1990). Secondary constituents in cell cultures of Hypericum perforatum and Hypericum maculatum. Planta Med, 56:634-637.
  • Kartnig T, Göbel I, Heydel B (1996). Production of hypericin, pseudohypericin and flavonoids in cell cultures of various Hypericum species and their chemotypes. Planta Med. 62:51-53.
  • Kirakosyan A, Hayashi H, Inoue K, Charchoglyan A, Vardapetyan H, Yammoto H (2001). The effect of cork pieces on pseudohypericin production in cells of Hypericum perforatum L. shoots. Russian J. Plant Physiol. 48:816-819.
  • Maciel GB, Moura M (2000). Multiplication Strategies for Hypericum foliosum Aiton, an Endemic Azorean Species. In Vitro Cell, and Develop. Biol. - Plant 36:3.
  • Matsuhisa M, Shikishima Y, Takaishi Y, Honda G, Ito M, Takeda Y, Shibata H, Higuti T, Kodzhimatov OK Ashurmetov O (2002). Benzoylphloroglucinol derivatives from Hypericum scabrum. JNat. Prod. 65:290-294.
  • Mederos MS (1991). In vitro growth and multiplication of Hypericum canariense L.ActaHortic. 289:133-135.
  • Moura M (1998). Conservation of Hypericum foliosura Aiton, an endemic Azorean species, by micropropagation. In Vitro Cell Dev. Biol. Plant. 34:244-248.
  • Murashige T Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497.
  • Pretto FR Santarem ER (2000). Callus formation and plant regeneration from Hypericum perforatum L. leaves. Plant Cell Tis. Org. Cult. 67:107-113.
  • Steel, R. G., and J. H. Torrie. 1980. Principles and Procedures of Statistics.McGraw- Hill Book Co., New York.
  • Tanker N (1971). Studies on Hypericum scabrum L. Journal of Faculty of Pharmacy of Ankara University. 1: 10-15.
  • Travis, SW, Bais HP, Vivanco JM (2002). Jasmonic acid-induced hypericin production in cell suspension cultures of Hypericum perforatum L. (St. John's wort). Phytochemistry. 60:289-293.
  • Yazaki K, Okuda T (1990). Procyanins in callus and multiple shoots of Hypericum erectum. Planta Med. 56:490-491.
  • Zobayed SMA, Saxena PK (2003). In vitro-grown roots: a superior explant for prolific shoot regeneration of St. John's wort (Hypericum perforatum L. cv. "New Stem") in a temporary immersion bioreactor. Plant Sci. 165:463-470.
  • Zobayed SMA, Murch SJ, Rupasinghe HPV, Saxena PK (2004) In vitro production and chemical characterization of St. John's wort (Hypericum perforatum L. cv. 'New Stem'). Plant Sci. 166: 333-340.
APA AYAN A, ÇIRAK C (2005). Callus induction in some hypericum species growing in Turkey. , 23 - 28.
Chicago AYAN Ali Kemal,ÇIRAK CÜNEYT Callus induction in some hypericum species growing in Turkey. (2005): 23 - 28.
MLA AYAN Ali Kemal,ÇIRAK CÜNEYT Callus induction in some hypericum species growing in Turkey. , 2005, ss.23 - 28.
AMA AYAN A,ÇIRAK C Callus induction in some hypericum species growing in Turkey. . 2005; 23 - 28.
Vancouver AYAN A,ÇIRAK C Callus induction in some hypericum species growing in Turkey. . 2005; 23 - 28.
IEEE AYAN A,ÇIRAK C "Callus induction in some hypericum species growing in Turkey." , ss.23 - 28, 2005.
ISNAD AYAN, Ali Kemal - ÇIRAK, CÜNEYT. "Callus induction in some hypericum species growing in Turkey". (2005), 23-28.
APA AYAN A, ÇIRAK C (2005). Callus induction in some hypericum species growing in Turkey. Turkish Journal of Field Crops, 10(1), 23 - 28.
Chicago AYAN Ali Kemal,ÇIRAK CÜNEYT Callus induction in some hypericum species growing in Turkey. Turkish Journal of Field Crops 10, no.1 (2005): 23 - 28.
MLA AYAN Ali Kemal,ÇIRAK CÜNEYT Callus induction in some hypericum species growing in Turkey. Turkish Journal of Field Crops, vol.10, no.1, 2005, ss.23 - 28.
AMA AYAN A,ÇIRAK C Callus induction in some hypericum species growing in Turkey. Turkish Journal of Field Crops. 2005; 10(1): 23 - 28.
Vancouver AYAN A,ÇIRAK C Callus induction in some hypericum species growing in Turkey. Turkish Journal of Field Crops. 2005; 10(1): 23 - 28.
IEEE AYAN A,ÇIRAK C "Callus induction in some hypericum species growing in Turkey." Turkish Journal of Field Crops, 10, ss.23 - 28, 2005.
ISNAD AYAN, Ali Kemal - ÇIRAK, CÜNEYT. "Callus induction in some hypericum species growing in Turkey". Turkish Journal of Field Crops 10/1 (2005), 23-28.