The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos

Objective: As an anionic surfactant, sodium lauryl sulphate (SLS), is used as an emulsifying agent in toothpastes as well as many different household cleaning products. Many toothpastes contain SLS and it is responsible for the formation of foam when brushing. However SLS may also irritate sensitive teeth and gums. Lipid peroxidation (LPO) is defined as an autoxidation process that is induced through the attack of free oxygen radicals leading to oxidative stress in the pathophysiology of various diseases. LPO causes the formation of highly reactive products including different aldehydes, ketones, and alkanes serving as biomarkers of LPO. In our study we aimed to expose zebrafish embryos to SLS and determine LPO in SLS exposed zebrafish embryos. Material and Method: Zebrafish embryos that were dividing normally and which had spherical shapes were chosen and they were exposed to SLS both in low and high concentrations in well plates for 72 hours. Rates of mortality and hatching were determined. The levels of malondialdehyde were evaluated using the Yagi's method as the end products of LPO in the form of thiobarbituric acid reactive substances. Results: Our findings showed LPO increased significantly in both low SLS (p<0.05) and high dose SLS (p<0.05) exposed zebrafish embryos when they were compared to the control group. Conclusion: It may be suggested that LPO is an early indicator of exposure to SLS during embryogenesis and further studies are required to confirm this finding.

Anahtar Kelime:

Sodyum Lauril Sülfatın Gelişmekte Olan Zebra Balığı Embriyolarında Biyouyumluluğu

Öz:

Amaç: Sodyum lauril sülfat (SLS), ev temizlik ürünleri ve diş macun larında emülsifiye edici temizlik maddesi olarak kullanılan anyonikbir yüzey aktif maddedir. Birçok diş macunu fırçalarken köpük olu şumuna yol açan SLS içerir. Ancak SLS hassas diş ve diş etlerini tahrişedebilir. Lipid peroksidasyonu (LPO), çeşitli hastalıkların patofizyo lojisinde oksidatif strese yol açan serbest radikallerin saldırısı ilebaşlatılan bir otooksidasyon sürecidir. LPO, LPO'nun biyobelirteçleriolarak görev yapan farklı aldehitler, ketonlar, alkanlar dahil olmaküzere reaktif ürünlerin oluşumuna neden olur. Çalışmamızın amacı,SLS'ye maruz kalan zebra balığı embriyolarında oluşan LPO'yu de ğerlendirmektir. Gereç ve Yöntem: Normal olarak bölünen küresel embriyolar, 72saat boyunca plaka kuyucuklarında düşük ve yüksek dozda SLS'yemaruz bırakılmıştır. Mortalite ve kuluçkadan çıkma oranları belir lenmiştir. Tiyobarbitürik asit reaktif maddeler olarak LPO'nun sonürünü olan malondialdehit (MDA) düzeyini belirlemek için Yagiyöntemi kullanılmıştır. Bulgular: Bulgularımız, hem düşük SLS (p<0,05) hem de yüksekdoz SLS’ye (p<0,05) maruz kalan zebra balığı embriyolarında kont rol grubu ile karşılaştırıldığında LPO'nun önemli ölçüde arttığınıgöstermiştir. Sonuç: LPO'nun embriyogenez sırasında SLS'ye maruz kalmanınerken bir göstergesi olduğu öne sürülebilir ve bu bulguyu doğru lamak için farklı çalışmaların yapılması gerektiği düşünülmektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Diğer Erişim Türü: Erişime Açık

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APA	MEŞELİ S, KAPLAN G, Cansız D, Ustundag U, Ünal İ, Emekli-Alturfan E, Yanikoglu F, Tagtekin D (2021). The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos. , 67 - 72. 10.26650/experimed.2021.953435
Chicago	MEŞELİ Simge,KAPLAN Gül,Cansız Derya,Ustundag Unsal Veli,Ünal İsmail,Emekli-Alturfan Ebru,Yanikoglu Funda,Tagtekin Dilek The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos. (2021): 67 - 72. 10.26650/experimed.2021.953435
MLA	MEŞELİ Simge,KAPLAN Gül,Cansız Derya,Ustundag Unsal Veli,Ünal İsmail,Emekli-Alturfan Ebru,Yanikoglu Funda,Tagtekin Dilek The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos. , 2021, ss.67 - 72. 10.26650/experimed.2021.953435
AMA	MEŞELİ S,KAPLAN G,Cansız D,Ustundag U,Ünal İ,Emekli-Alturfan E,Yanikoglu F,Tagtekin D The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos. . 2021; 67 - 72. 10.26650/experimed.2021.953435
Vancouver	MEŞELİ S,KAPLAN G,Cansız D,Ustundag U,Ünal İ,Emekli-Alturfan E,Yanikoglu F,Tagtekin D The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos. . 2021; 67 - 72. 10.26650/experimed.2021.953435
IEEE	MEŞELİ S,KAPLAN G,Cansız D,Ustundag U,Ünal İ,Emekli-Alturfan E,Yanikoglu F,Tagtekin D "The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos." , ss.67 - 72, 2021. 10.26650/experimed.2021.953435
ISNAD	MEŞELİ, Simge vd. "The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos". (2021), 67-72. https://doi.org/10.26650/experimed.2021.953435

APA	MEŞELİ S, KAPLAN G, Cansız D, Ustundag U, Ünal İ, Emekli-Alturfan E, Yanikoglu F, Tagtekin D (2021). The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos. EXPERIMED, 11(2), 67 - 72. 10.26650/experimed.2021.953435
Chicago	MEŞELİ Simge,KAPLAN Gül,Cansız Derya,Ustundag Unsal Veli,Ünal İsmail,Emekli-Alturfan Ebru,Yanikoglu Funda,Tagtekin Dilek The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos. EXPERIMED 11, no.2 (2021): 67 - 72. 10.26650/experimed.2021.953435
MLA	MEŞELİ Simge,KAPLAN Gül,Cansız Derya,Ustundag Unsal Veli,Ünal İsmail,Emekli-Alturfan Ebru,Yanikoglu Funda,Tagtekin Dilek The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos. EXPERIMED, vol.11, no.2, 2021, ss.67 - 72. 10.26650/experimed.2021.953435
AMA	MEŞELİ S,KAPLAN G,Cansız D,Ustundag U,Ünal İ,Emekli-Alturfan E,Yanikoglu F,Tagtekin D The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos. EXPERIMED. 2021; 11(2): 67 - 72. 10.26650/experimed.2021.953435
Vancouver	MEŞELİ S,KAPLAN G,Cansız D,Ustundag U,Ünal İ,Emekli-Alturfan E,Yanikoglu F,Tagtekin D The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos. EXPERIMED. 2021; 11(2): 67 - 72. 10.26650/experimed.2021.953435
IEEE	MEŞELİ S,KAPLAN G,Cansız D,Ustundag U,Ünal İ,Emekli-Alturfan E,Yanikoglu F,Tagtekin D "The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos." EXPERIMED, 11, ss.67 - 72, 2021. 10.26650/experimed.2021.953435
ISNAD	MEŞELİ, Simge vd. "The Biocompatibility of Sodium Lauryl Sulphate onDeveloping Zebrafish Embryos". EXPERIMED 11/2 (2021), 67-72. https://doi.org/10.26650/experimed.2021.953435