Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa

Kaskatepe, Banu; Erol, Hilal Başak

doi:10.29228/jrp.31

Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa

Hilal Basak EROL, (Ankara Üniversitesi, Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, Yenimahalle, Ankara, Türkiye)

BANU KAŞKATEPE (Ankara Üniversitesi, Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, Yenimahalle, Ankara, Türkiye)

Journal of research in pharmacy (online)

1 0

Yıl: 2021 Cilt: 25 Sayı: 4 Sayfa Aralığı: 407 - 414 Metin Dili: İngilizce DOI: 10.29228/jrp.31 İndeks Tarihi: 08-11-2021

Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa

Öz:

The result of a crucial rise in the prevalence of antibiotic resistance of bacteria, and the development of an inadequate number of new antibiotics, over the last decade a marked increase in interest in the studies of phages has been observed. It was aimed to determine the commercial preparation including Pyo-bacteriophage and Intestibacteriophage and compare their efficiency with isolated bacteriophage vB_Pa01 against carbapenem resistant Pseudomonas aeruginosa. Susceptibility to the bacteriophages present in each cocktail was tested using the spot test. A total of 126 carbapenem-resistant P. aeruginosa isolates were included in this test. Bacteriophage susceptibilities of each isolate were tested by spot test method. Confluent, semi confluent, opaque lysis was considered a sensitivity. The absence of any lysis is reported as resistance. The lytic activity of the bacteriophage was found 46% (58/126) Pyobacteriophage and 36.5% (46/126) Intesti-bacteriophage and 63.5% (80/126) vB_Pa01 bacteriophage. The most effective bacteriophage preparation was the vB_Pa01 bacteriophage (63.5%) and the least effective one was Intesti-bacteriophage (36.5%). 95 (75.4%) of 126 carbapenem resistant. This is the first study that investigated two commercial bacteriophage cocktails against carbapenem resistant P. aeruginosa. The higher efficacy of vB_Pa01 bacteriophage is promising for creating an alternative preparation especially in the treatment of infections caused by MDR pathogens that are difficult to treat.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Erol H, Kaskatepe B (2021). Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa. , 407 - 414. 10.29228/jrp.31
Chicago	Erol Hilal Başak,Kaskatepe Banu Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa. (2021): 407 - 414. 10.29228/jrp.31
MLA	Erol Hilal Başak,Kaskatepe Banu Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa. , 2021, ss.407 - 414. 10.29228/jrp.31
AMA	Erol H,Kaskatepe B Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa. . 2021; 407 - 414. 10.29228/jrp.31
Vancouver	Erol H,Kaskatepe B Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa. . 2021; 407 - 414. 10.29228/jrp.31
IEEE	Erol H,Kaskatepe B "Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa." , ss.407 - 414, 2021. 10.29228/jrp.31
ISNAD	Erol, Hilal Başak - Kaskatepe, Banu. "Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa". (2021), 407-414. https://doi.org/10.29228/jrp.31

APA	Erol H, Kaskatepe B (2021). Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa. Journal of research in pharmacy (online), 25(4), 407 - 414. 10.29228/jrp.31
Chicago	Erol Hilal Başak,Kaskatepe Banu Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa. Journal of research in pharmacy (online) 25, no.4 (2021): 407 - 414. 10.29228/jrp.31
MLA	Erol Hilal Başak,Kaskatepe Banu Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa. Journal of research in pharmacy (online), vol.25, no.4, 2021, ss.407 - 414. 10.29228/jrp.31
AMA	Erol H,Kaskatepe B Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa. Journal of research in pharmacy (online). 2021; 25(4): 407 - 414. 10.29228/jrp.31
Vancouver	Erol H,Kaskatepe B Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa. Journal of research in pharmacy (online). 2021; 25(4): 407 - 414. 10.29228/jrp.31
IEEE	Erol H,Kaskatepe B "Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa." Journal of research in pharmacy (online), 25, ss.407 - 414, 2021. 10.29228/jrp.31
ISNAD	Erol, Hilal Başak - Kaskatepe, Banu. "Comparison of the in vitro efficacy of commercialbacteriophage cocktails and isolated bacteriophagevB_Pa01 against carbapenem resistant nosocomialPseudomonas aeruginosa". Journal of research in pharmacy (online) 25/4 (2021), 407-414. https://doi.org/10.29228/jrp.31