RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION
Yıl: 2020 Cilt: 8 Sayı: 2 Sayfa Aralığı: 264 - 270 Metin Dili: İngilizce DOI: 10.37696/nkmj.687468 İndeks Tarihi: 14-11-2021
RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION
Öz: Aim: L-2-hydroxyglutaric aciduria (L2HGA) which is autosomal recessive and characterized by psychomotor retardation, cerebellarataxia, variable macrocephaly, and epilepsy is a rarely seen neurometabolic disease. The disease is biochemically identified by slightlyincreased L-2HGA levels in urine, cerebrospinal fluid, and plasma.Materials and Methods: A sensitive and rapid capillary electrophoretic technique was used for the determination of L2HGA in urine.Since the L2HGA lacks of chromophore, an indirect UV detection method was applied.Results: The running electrolyte consisted of 10 mmol/L 2,6-pyridinedicarboxylic acid (PDC) at pH 5.6 containing 0.1 mmol/L ofcetyltrimethylammonium bromide (CTAB). PDC was chosen because of its good chromophoric property for indirect UV detection. CTABwas used as electro-osmotic flow (EOF) reversal. The method was well-validated in the selected conditions. The detection limit of themethod L2HGA was 1.16 μmol/L. The migration time of L2HGA was less than 2.0 min.Conclusion: The developed method was applied to the urine samples collected from 16 patients who suffer from L-2-hydroxyglutaricaciduria and 4 healthy volunteers.
Anahtar Kelime: İdrar Örneklerinde L-2-Hidroksiglutarik Asitin Kapiler Elektroforez-İndirekt UV Dedeksiyon ile Hızlı Tayini
Öz: Amaç: L-2 hidroksiglutarik asidüri (L2HGA) otozomal resesif geçişli, psikomotor gerilik, serebellar ataksi ve değişken makrosefali veya epilepsi ile karakterize edilen nadir görülen nörömetabolik bir hastalıktır. Hastalık biyokimyasal olarak idrarda, beyin omurilik sıvısında ve çok az miktarda plazmada artmış L-2HGA seviyeleri ile tanımlanır. Materyal ve Metot: Bu çalışmada, idrarda L-2-hidroksiglutarik asitin tayini için hızlı ve hassas bir kapiler elektroforez yöntemi geliştirilmiştir. L-2-hidroksiglutarik asit kromofor gruba sahip olmadığı için, indirekt UV deteksiyon metodu uygulanmıştır. Bulgular: Çalışma tamponu pH 5.6’da 0.1 mmol/L setiltrimetilamonyum bromür (CTAB) içeren 10 mmol/L 2,6-piridindikarboksilik asitten (PDC) oluşmaktadır. PDC iyi bir kromoforik özellik göstermesinden dolayı indirekt deteksiyon için uygundur. CTAB elektro-osmotik akışı (EOF) ters çevirmek için kullanılmıştır. L-2-hidroksiglutarik asit için tayin limiti 1.16 μmol/L’dir. Geliş zamanı ise 2.0 dakikadan daha az bir süredir. Sonuç: Geliştirilen metod 16 L-2-hidroksiglutarik asidüri hastasından ve 4 sağlıklı gönüllüden toplanan idrar örneklerine uygulanmıştır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | CANSEVER M, Kalaycıoğlu Z, ERİM F, ZÜBARİOĞLU T, KIYKIM E, ZEYBEK A (2020). RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION. , 264 - 270. 10.37696/nkmj.687468 |
| Chicago | CANSEVER Mehmet Şerif,Kalaycıoğlu Zeynep,ERİM Fatma Bedia,ZÜBARİOĞLU Tanyel,KIYKIM Ertuğrul,ZEYBEK Ayşe Çiğdem AKTUĞLU RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION. (2020): 264 - 270. 10.37696/nkmj.687468 |
| MLA | CANSEVER Mehmet Şerif,Kalaycıoğlu Zeynep,ERİM Fatma Bedia,ZÜBARİOĞLU Tanyel,KIYKIM Ertuğrul,ZEYBEK Ayşe Çiğdem AKTUĞLU RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION. , 2020, ss.264 - 270. 10.37696/nkmj.687468 |
| AMA | CANSEVER M,Kalaycıoğlu Z,ERİM F,ZÜBARİOĞLU T,KIYKIM E,ZEYBEK A RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION. . 2020; 264 - 270. 10.37696/nkmj.687468 |
| Vancouver | CANSEVER M,Kalaycıoğlu Z,ERİM F,ZÜBARİOĞLU T,KIYKIM E,ZEYBEK A RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION. . 2020; 264 - 270. 10.37696/nkmj.687468 |
| IEEE | CANSEVER M,Kalaycıoğlu Z,ERİM F,ZÜBARİOĞLU T,KIYKIM E,ZEYBEK A "RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION." , ss.264 - 270, 2020. 10.37696/nkmj.687468 |
| ISNAD | CANSEVER, Mehmet Şerif vd. "RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION". (2020), 264-270. https://doi.org/10.37696/nkmj.687468 |
| APA | CANSEVER M, Kalaycıoğlu Z, ERİM F, ZÜBARİOĞLU T, KIYKIM E, ZEYBEK A (2020). RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION. Namık Kemal Tıp Dergisi, 8(2), 264 - 270. 10.37696/nkmj.687468 |
| Chicago | CANSEVER Mehmet Şerif,Kalaycıoğlu Zeynep,ERİM Fatma Bedia,ZÜBARİOĞLU Tanyel,KIYKIM Ertuğrul,ZEYBEK Ayşe Çiğdem AKTUĞLU RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION. Namık Kemal Tıp Dergisi 8, no.2 (2020): 264 - 270. 10.37696/nkmj.687468 |
| MLA | CANSEVER Mehmet Şerif,Kalaycıoğlu Zeynep,ERİM Fatma Bedia,ZÜBARİOĞLU Tanyel,KIYKIM Ertuğrul,ZEYBEK Ayşe Çiğdem AKTUĞLU RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION. Namık Kemal Tıp Dergisi, vol.8, no.2, 2020, ss.264 - 270. 10.37696/nkmj.687468 |
| AMA | CANSEVER M,Kalaycıoğlu Z,ERİM F,ZÜBARİOĞLU T,KIYKIM E,ZEYBEK A RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION. Namık Kemal Tıp Dergisi. 2020; 8(2): 264 - 270. 10.37696/nkmj.687468 |
| Vancouver | CANSEVER M,Kalaycıoğlu Z,ERİM F,ZÜBARİOĞLU T,KIYKIM E,ZEYBEK A RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION. Namık Kemal Tıp Dergisi. 2020; 8(2): 264 - 270. 10.37696/nkmj.687468 |
| IEEE | CANSEVER M,Kalaycıoğlu Z,ERİM F,ZÜBARİOĞLU T,KIYKIM E,ZEYBEK A "RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION." Namık Kemal Tıp Dergisi, 8, ss.264 - 270, 2020. 10.37696/nkmj.687468 |
| ISNAD | CANSEVER, Mehmet Şerif vd. "RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION". Namık Kemal Tıp Dergisi 8/2 (2020), 264-270. https://doi.org/10.37696/nkmj.687468 |
