TY  - JOUR
TI  - RAPID DETERMINATION OF L-2-HYDOXYGLUTARIC ACID IN URINE SAMPLES BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION
AB  - Aim: L-2-hydroxyglutaric aciduria (L2HGA) which is autosomal recessive and characterized by psychomotor retardation, cerebellarataxia, variable macrocephaly, and epilepsy is a rarely seen neurometabolic disease. The disease is biochemically identified by slightlyincreased L-2HGA levels in urine, cerebrospinal fluid, and plasma.Materials and Methods: A sensitive and rapid capillary electrophoretic technique was used for the determination of L2HGA in urine.Since the L2HGA lacks of chromophore, an indirect UV detection method was applied.Results: The running electrolyte consisted of 10 mmol/L 2,6-pyridinedicarboxylic acid (PDC) at pH 5.6 containing 0.1 mmol/L ofcetyltrimethylammonium bromide (CTAB). PDC was chosen because of its good chromophoric property for indirect UV detection. CTABwas used as electro-osmotic flow (EOF) reversal. The method was well-validated in the selected conditions. The detection limit of themethod L2HGA was 1.16 μmol/L. The migration time of L2HGA was less than 2.0 min.Conclusion: The developed method was applied to the urine samples collected from 16 patients who suffer from L-2-hydroxyglutaricaciduria and 4 healthy volunteers.
AU  - KIYKIM, Ertuğrul
AU  - ZÜBARİOĞLU, Tanyel
AU  - Kalaycıoğlu, Zeynep
AU  - ERİM, Fatma Bedia
AU  - ZEYBEK, Ayşe Çiğdem AKTUĞLU
AU  - CANSEVER, Mehmet Şerif
DO  - 10.37696/nkmj.687468
PY  - 2020
JO  - Namık Kemal Tıp Dergisi
VL  - 8
IS  - 2
SN  - 2587-0262
SP  - 264
EP  - 270
DB  - TRDizin
UR  - http://search/yayin/detay/460118
ER  -