Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study

Şermin YILDIRIM, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Samsun, Türkiye)
Semra GÜMÜŞOVA (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Samsun, Türkiye)
Aquaculture Studies
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Yıl: 2020 Cilt: 20 Sayı: 2 Sayfa Aralığı: 113 - 119 Metin Dili: İngilizce DOI: 10.4194/2618-6381-v20_2_05 İndeks Tarihi: 19-11-2021

Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study

Öz:
Infectious Pancreatic Necrosis Virus (IPNV) is a viral agent that causes significant losses in salmonid fish in the rainbow trout and salmon farming sectors. The present study aimed to examine of quercetin effects on (IPNV) replication in Rainbow trout gonad (RTG-2) cell cultures. Half-maximal (50%) cytostatic concentration (CC 50) of quercetin were detected in the RTG-2 cell culture by cytotoxicity test with Cell Counting Kit-8 (CCK-8, Sigma-Aldrich) (n=3). Titration, cytopathogenic effect reduction, and qRT-PCR tests were used to determine the effect of quercetin on IPNV replication in RTG-2 cell culture. At the end of the cytotoxicity test, the non-toxic dose of quercetin was determined. Besides that, 50 μmol/L quercetin decreased the titer of IPNV in RTG-2 cells from 10-7 to 10-5, at the same time, the same dose of quercetin reduced the viral load to 40%. We concluded that quercetin is a potent antioxidant agent for IPNV infections and it can be an option in the treatment of the IPN disease.
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APA Yildirim S, Okur Gumusova S (2020). Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study. , 113 - 119. 10.4194/2618-6381-v20_2_05
Chicago Yildirim Sermin,Okur Gumusova Semra Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study. (2020): 113 - 119. 10.4194/2618-6381-v20_2_05
MLA Yildirim Sermin,Okur Gumusova Semra Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study. , 2020, ss.113 - 119. 10.4194/2618-6381-v20_2_05
AMA Yildirim S,Okur Gumusova S Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study. . 2020; 113 - 119. 10.4194/2618-6381-v20_2_05
Vancouver Yildirim S,Okur Gumusova S Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study. . 2020; 113 - 119. 10.4194/2618-6381-v20_2_05
IEEE Yildirim S,Okur Gumusova S "Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study." , ss.113 - 119, 2020. 10.4194/2618-6381-v20_2_05
ISNAD Yildirim, Sermin - Okur Gumusova, Semra. "Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study". (2020), 113-119. https://doi.org/10.4194/2618-6381-v20_2_05
APA Yildirim S, Okur Gumusova S (2020). Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study. Aquaculture Studies, 20(2), 113 - 119. 10.4194/2618-6381-v20_2_05
Chicago Yildirim Sermin,Okur Gumusova Semra Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study. Aquaculture Studies 20, no.2 (2020): 113 - 119. 10.4194/2618-6381-v20_2_05
MLA Yildirim Sermin,Okur Gumusova Semra Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study. Aquaculture Studies, vol.20, no.2, 2020, ss.113 - 119. 10.4194/2618-6381-v20_2_05
AMA Yildirim S,Okur Gumusova S Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study. Aquaculture Studies. 2020; 20(2): 113 - 119. 10.4194/2618-6381-v20_2_05
Vancouver Yildirim S,Okur Gumusova S Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study. Aquaculture Studies. 2020; 20(2): 113 - 119. 10.4194/2618-6381-v20_2_05
IEEE Yildirim S,Okur Gumusova S "Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study." Aquaculture Studies, 20, ss.113 - 119, 2020. 10.4194/2618-6381-v20_2_05
ISNAD Yildirim, Sermin - Okur Gumusova, Semra. "Effect of Quercetin on Infectious Pancreatic Necrosis Virus: inVitro Replication Study". Aquaculture Studies 20/2 (2020), 113-119. https://doi.org/10.4194/2618-6381-v20_2_05
Sayfa Oluşturulma Tarihi
23-04-2024 20:11

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