Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions

Dönmez, Gönül; KOÇBERBER KILIÇ, NUR

doi:10.15671/hjbc.696762

Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions

Nur KOÇBERBER KILIÇ, (Ankara Üniversitesi, Biyoloji Bölümü, Ankara, Türkiye)

Gönül DÖNMEZ (Ankara Üniversitesi, Biyoloji Bölümü, Ankara, Türkiye)

Hacettepe Journal of Biology and Chemistry

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Yıl: 2021 Cilt: 49 Sayı: 3 Sayfa Aralığı: 233 - 242 Metin Dili: İngilizce DOI: 10.15671/hjbc.696762 İndeks Tarihi: 20-11-2021

Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions

Öz:

In this report, bioactive properties of 7 thermophile cyanobacteria isolated from thermal springs in Turkey were investigated. Of these, Strain H2 having the highest antimicrobial activity was identified as Cyanobacterium aponium. Bioactive character of cyanobacterial biomass was investigated with regards to different nitrogen concentrations (0.5 g/L, 1.0 g/L, 1.5 g/L, and 2.0 g/L), light intensities (1200lx, 2400 lx, 3600 lx, and 4800 lx), incubation periods (7 d, 14 d, 21 d, and 28 d), and temperatures (30°C, 40°C, 45°C, and 50°C). It was observed that the effectiveness of bioactive substances produced by cyanobacteria was induced by stress conditions. When C. aponinum was exposed to high light intensity or temperature, cyanobacteria produced more efficient bioactive compounds then other environmental conditions tested. The highest antimicrobial activity was found against E. coli 0157:H7 ATCC 35150 with biomass extracts obtained when cyanobacterium cultivated in media with 1.0 g/L nitrogen, at 45°C, under 3600 lx illumination after incubation for 14 days. For the first time with such an approach as in the current study, production of bioactive compounds by a thermophilic C. aponinum and optimization of the environmental conditions to obtain the most efficient biologically active compounds was investigated.

Anahtar Kelime:

Stres Koşulları Altında Termofil Cyanobacterium aponinum Biyoaktif Bileşik Aktivitesinin İndüklenmesi

Öz:

Bu çalışmada, Türkiye’de kaplıcalardan izole edilen 7 termofil siyanobakterinin biyoaktif özellikleri araştırılmıştır. Bunlar dan en yüksek antimikrobiyel aktiviteye sahip olan Suş H2, Cyanobacterium aponium olarak tanılanmıştır. Siyanobak teriyel biyokütlenin biyoaktif karakteri, farklı azot konsantrasyonları (0.5 g/L, 1.0 g/L, 1.5 g/L ve 2.0 g/L), ışık yoğunlukları(1200lx, 2400 lx, 3600 lx ve 4800 lx), inkübasyon süreleri (7 gün, 14 gün, 21 gün ve 28 gün) ve sıcaklıklar (30°C, 40°C, 45°Cve 50°C) açısından araştırılmıştır. Siyanobakteriler tarafından üretilen biyoaktif maddelerin etkinliğinin stres koşulları tara fından tetiklendiği gözlenmiştir. C. aponinum yüksek ışık yoğunluğuna veya sıcaklığa maruz kaldığında, siyanobakteriler testedilen diğer çevresel koşullardan daha verimli biyoaktif bileşikler üretmiştir. En yüksek antimikrobiyel aktivite, siyanobakteri1.0 g/L azot içeren bir ortamda, 3600 lx ışık şiddeti altında, 45°C’de 14 gün boyunca inkübasyondan sonra elde edilen biyo kütleden alınan ekstraktlar ile E. coli 0157: H7 ATCC 35150’ye karşı bulunmuştur. Bu çalışmada ilk kez böyle bir yaklaşımla,termofilik C. aponinum tarafından biyoaktif bileşiklerin üretilmesi ve en etkin biyoaktif bileşikleri elde etmek için çevreselkoşulların optimizasyonu araştırılmıştır.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KOÇBERBER KILIÇ N, Dönmez G (2021). Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions. , 233 - 242. 10.15671/hjbc.696762
Chicago	KOÇBERBER KILIÇ NUR,Dönmez Gönül Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions. (2021): 233 - 242. 10.15671/hjbc.696762
MLA	KOÇBERBER KILIÇ NUR,Dönmez Gönül Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions. , 2021, ss.233 - 242. 10.15671/hjbc.696762
AMA	KOÇBERBER KILIÇ N,Dönmez G Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions. . 2021; 233 - 242. 10.15671/hjbc.696762
Vancouver	KOÇBERBER KILIÇ N,Dönmez G Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions. . 2021; 233 - 242. 10.15671/hjbc.696762
IEEE	KOÇBERBER KILIÇ N,Dönmez G "Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions." , ss.233 - 242, 2021. 10.15671/hjbc.696762
ISNAD	KOÇBERBER KILIÇ, NUR - Dönmez, Gönül. "Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions". (2021), 233-242. https://doi.org/10.15671/hjbc.696762

APA	KOÇBERBER KILIÇ N, Dönmez G (2021). Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions. Hacettepe Journal of Biology and Chemistry, 49(3), 233 - 242. 10.15671/hjbc.696762
Chicago	KOÇBERBER KILIÇ NUR,Dönmez Gönül Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions. Hacettepe Journal of Biology and Chemistry 49, no.3 (2021): 233 - 242. 10.15671/hjbc.696762
MLA	KOÇBERBER KILIÇ NUR,Dönmez Gönül Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions. Hacettepe Journal of Biology and Chemistry, vol.49, no.3, 2021, ss.233 - 242. 10.15671/hjbc.696762
AMA	KOÇBERBER KILIÇ N,Dönmez G Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions. Hacettepe Journal of Biology and Chemistry. 2021; 49(3): 233 - 242. 10.15671/hjbc.696762
Vancouver	KOÇBERBER KILIÇ N,Dönmez G Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions. Hacettepe Journal of Biology and Chemistry. 2021; 49(3): 233 - 242. 10.15671/hjbc.696762
IEEE	KOÇBERBER KILIÇ N,Dönmez G "Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions." Hacettepe Journal of Biology and Chemistry, 49, ss.233 - 242, 2021. 10.15671/hjbc.696762
ISNAD	KOÇBERBER KILIÇ, NUR - Dönmez, Gönül. "Bioactive Compound Activity Inducement of Thermophile Cyanobacteriumaponinum Under Stress Conditions". Hacettepe Journal of Biology and Chemistry 49/3 (2021), 233-242. https://doi.org/10.15671/hjbc.696762