Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells
Yıl: 2021 Cilt: 14 Sayı: 1 Sayfa Aralığı: 82 - 87 Metin Dili: İngilizce DOI: 10.46309/biodicon.2021.888000 İndeks Tarihi: 21-11-2021
Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells
Öz: Prostate cancer is one of the most prevalent cancer types in males with a percentages of 28% worldwide. Thiscancer type comprises approximately 37% of the cancer incidences in males in Turkey. Recent cancer investigations arefocused on natural agents with good potent for cancer therapy. Escin is one of the most investigated agents of that kindbut its effects on human prostate cancer cell’s morphology is not investigated in detail, yet. Thus, the aim of this studyis to investigate the antiproliferative, cytotoxic and proapoptotic effects of escin, on prostate cancer cells Du-145.Cytotoxicity of escin on prostate cancer cells was intestigated by using sulforhodamine B (SRB) assay and viabilitypercentages and IC50 value were detected from the elisa reader (BioTek Synergy HTX) results. For morphologicalchanges, Du-145 cells treated with the IC50 value of escin were evaluated under a confocal microscope (Leica, TCS SP5II, Germany). Apoptosis profiles of cells were investigated by flow cytometry. According to the SRB findings escinreduced the viability of prostate cancer cells in dose-dependent manner and the IC50 value was detected as 30.48 µM for24 hours. On the confocal microscopy results it was confirmed that escin significantly changed the morphology of thetreated cells as disintegrated and deformed nuclei, chromatin condensation, fragmentations in the cytoskeleton alsoshrinkage of prostate cells. Annexin-V technique indicated the apoptotic cell death trigered by escin in Du-145 cells.Based on the study results, it was concluded that escin changed the morphology of prostate cancer cells and inducedapoptosis on prostate cancer cells.
Anahtar Kelime: İnsan prostat kanseri hücreleri üzerinde escinin apoptozis ve morfolojik değişiklikleri tetikleyici sitotoksik etkileri
Öz: Prostat kanseri, dünya çapında % 28'lik bir oranla erkeklerde en sık görülen kanser türlerinden biridir. Bu kanser türü, Türkiye'de erkeklerde görülen kanser vakalarının yaklaşık % 37'sini oluşturmaktadır. Son kanser araştırmaları, kanser tedavisi için potansiyele sahip doğal ajanlara odaklanmıştır. Escin, bu özelliğinden dolayı en çok araştırılan ajanlardan biridir, ancak insan prostat kanseri hücresinin morfolojisi üzerindeki etkileri henüz ayrıntılı olarak araştırılmamıştır. Bu nedenle, bu çalışmanın amacı escinin Du-145 insan prostat kanseri hücreleri üzerindeki antiproliferatif, sitotoksik ve proapoptotik etkilerini araştırmaktır. Esinin prostat kanseri hücreleri üzerindeki sitotoksisitesi sulforhodamine B (SRB) testi ile incelenmiştir ve eliza plaka okuyucu (BioTek Synergy HTX) sonuçlarından canlılık yüzdeleri ve IC50 değeri saptanmıştır. Morfolojik değişiklikler için escinin IC50 değeri ile inkübe edilmiş olan Du-145 hücreleri, bir konfokal mikroskop (Leica, TCS SP5 II, Almanya) altında değerlendirilmiştir. Hücrelerin apoptotik profilleri akış sitometrisi ile araştırılmıştır. SRB bulgularına göre escin, prostat kanseri hücrelerinin canlılığını doza bağlı olarak azaltmış ve IC50 değeri 24 saat için 30,48 µM olarak tespit edilmiştir. Konfokal mikroskopi sonuçlarında, escin ile muamele edilmiş hücrelerin morfolojisinde, parçalanmış ve deforme olmuş çekirdekler, kromatin yoğunlaşması, hücre iskeletindeki fragmantasyonlar ve hücre büzüşmesi şeklinde önemli değişiklikler saptanmıştır. Anneksin-V tekniği, Du-145 hücrelerinde escinin apoptotik hücre ölümünü tetiklediğini göstermiştir. Çalışma sonuçlarına bakıldığında, escinin insan prostat kanseri hücrelerinde sitotoksik etki göstererek morfolojik değişikliklere neden olduğu ve apoptozisi indüklediği saptanmıştır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Vejselova Sezer C (2021). Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells. , 82 - 87. 10.46309/biodicon.2021.888000 |
| Chicago | Vejselova Sezer Canan Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells. (2021): 82 - 87. 10.46309/biodicon.2021.888000 |
| MLA | Vejselova Sezer Canan Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells. , 2021, ss.82 - 87. 10.46309/biodicon.2021.888000 |
| AMA | Vejselova Sezer C Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells. . 2021; 82 - 87. 10.46309/biodicon.2021.888000 |
| Vancouver | Vejselova Sezer C Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells. . 2021; 82 - 87. 10.46309/biodicon.2021.888000 |
| IEEE | Vejselova Sezer C "Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells." , ss.82 - 87, 2021. 10.46309/biodicon.2021.888000 |
| ISNAD | Vejselova Sezer, Canan. "Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells". (2021), 82-87. https://doi.org/10.46309/biodicon.2021.888000 |
| APA | Vejselova Sezer C (2021). Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells. Biological Diversity and Conservation, 14(1), 82 - 87. 10.46309/biodicon.2021.888000 |
| Chicago | Vejselova Sezer Canan Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells. Biological Diversity and Conservation 14, no.1 (2021): 82 - 87. 10.46309/biodicon.2021.888000 |
| MLA | Vejselova Sezer Canan Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells. Biological Diversity and Conservation, vol.14, no.1, 2021, ss.82 - 87. 10.46309/biodicon.2021.888000 |
| AMA | Vejselova Sezer C Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells. Biological Diversity and Conservation. 2021; 14(1): 82 - 87. 10.46309/biodicon.2021.888000 |
| Vancouver | Vejselova Sezer C Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells. Biological Diversity and Conservation. 2021; 14(1): 82 - 87. 10.46309/biodicon.2021.888000 |
| IEEE | Vejselova Sezer C "Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells." Biological Diversity and Conservation, 14, ss.82 - 87, 2021. 10.46309/biodicon.2021.888000 |
| ISNAD | Vejselova Sezer, Canan. "Cytotoxic impacts of escin via inducing apoptosis and morphological changes on human prostate cancer cells". Biological Diversity and Conservation 14/1 (2021), 82-87. https://doi.org/10.46309/biodicon.2021.888000 |
