Bee venom from honey bees (Apis Mellifera L.) is known to have many pharmacological andbiological properties. Melittin, a peptide consisting of 26 amino acids, is known as the main component ofbee venom. The study aims to develop a rapid capillary electrophoresis method for separating andquantifying melittin in honeybee venom. Since melittin is a basic peptide, it will adhere to the capillary wallduring separation. Two different methods were developed in this study for the capillary electrophoreticseparation of melittin. As a first approach, a low pH buffer system was used. For the second approach, thecapillary column was coated with a positively charged polymer (PEI). With both methods developed, themigration of melittin in the capillary was achieved by preventing wall adsorption. Melittin migrated in 6 minwhen the low-pH buffer system was applied, whereas its migration time is longer than 10 min in the PEIcoated capillary column. Thus, a low-pH buffer system was preferred for the analysis of the actual beevenom sample. 100 mmol L-1 phosphoric acid/sodium dihydrogen phosphate system at pH 1.55 waschosen as separation buffer. As a conclusion, a fast and reliable method was developed for thedetermination of melittin in honeybee venom. The method was applied to an Anatolian bee venom sampleto highlight the melittin amount. The melittin amount was found as 24.5 ± 3.4 g 100 g-1 in the bee venomsample.