COVID-19 infection caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) con tinues to affect people as a global threat, and the number of cases is increasing every day. Healthcareworkers who face potential COVID-19 exposure are at high risk of SARS-CoV-2 transmission. Estimatingthe prevalence of infection among healthcare professionals, determining the related risk factors andapplying effective infection control measures are essential for the continuity of the health system. Theaim of this study was to investigate the seroprevalence of SARS-CoV-2 among healthcare workers in ourhospital who have participated extensively in the monitoring of COVID-19 patients. In the study, theanti-SARS-CoV-2 IgG antibody test results of 774 healthcare workers between March 24, 2020, and Sep tember 10, 2020 were analyzed retrospectively. Age, sex, profession, and the status of being diagnosedwith COVID-19 before the antibody test were determined for the healthcare workers in the study. Whenthe anti-SARS-CoV-2 IgG antibody results were evaluated, it was determined that 57 healthcare workerswere positive, 708 healthcare workers were negative, and 9 healthcare workers were borderline. The se roprevalence among the workers of our hospital was found to be 7.4%. The antibody positivity rate was75.6% in individuals diagnosed with COVID-19 by SARS-CoV-2 PCR (polymerase chain reaction) and/orthoracic computed tomography and it was found to be 3.5% in individuals without the diagnosis. Thesemi-quantitative antibody index values of the healthcare workers who were seropositive and diagnosedwith COVID-19 before the test (n= 31) and those who did not (n= 26) were statistically compared anda significant difference was found between the two groups (p< 0.01). In our study, the highest sero positivity was observed among residents (12.3%) and among nurses (11.1%), respectively. When theseropositivity rates of the residents and the nurses were compared with other occupational groups, thedifferences were found to be statistically significant (p= 0.04, p= 0.04, respectively). In conclusion, theseroprevalence of SARS-CoV-2 was determined as 7.4% among healthcare workers in a tertiary hospitalwith high patient admissions during the COVID-19 pandemic. Considering that SARS-CoV-2 seropre valence was announced as 0.81% in the press release made by the Ministry of Health of Turkey in July2020, it is seen that the rate of seroprevalence among health care workers is significantly larger than thecommunity. Determination of the seroprevalence in the general population and large-scale studies areneeded for risk assessment in healthcare professionals.