L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa
Yıl: 2021 Cilt: 27 Sayı: 4 Sayfa Aralığı: 489 - 493 Metin Dili: İngilizce DOI: 10.9775/kvfd.2021.25756 İndeks Tarihi: 23-11-2021
L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa
Öz: The study aimed to evaluate the eff ects of L-carnitine on honey bee (Apis mellifera) drone spermatozoon at post-thaw. Semen samples obtained from sexually mature drones were pooled. Then, pooled semen was diluted with diff erent concentrations of L-carnitine (2.5 mM/ 5 mM/ 10 mM) and without L-carnitine (control) supplemented extenders. Motility, plasma membrane functional integrity, acrosomal integrity and mitochondrial function were negatively aff ected by the cryopreservation process but DNA integrity was not aff ected. Malondialdehyde (MDA) concentration was used for analyzing the membrane lipid peroxidation status and a better result was obtained in the LC5 group compared with the control group at post-thaw. L-carnitine groups had a positive eff ect on sperm motility, plasma membrane integrity, and mitochondrial function compared to the control group (P<0.05). Acrosomal integrity was better preserved in the LC5 group compared to the control group. The study shows that LC supplemented extenders have beneficial eff ects on honey bee drone sperm parameters. The results of the present study demonstrated the beneficial eff ect of using a 5 mMol LC supplemented extender.
Anahtar Kelime: L-Karnitin İlave Edilmiş Sulandırıcılar Bal Arısı (Apis mellifera) Spermatozoası’nın Çözdürme Sonrası Kalitesini Arttırır
Öz: Bu çalışmada, L-karnitinin eritme sonrası bal arısı (Apis mellifera) spermatozoonu üzerindeki etkilerini değerlendirme amaçlandı. Cinsel olarak olgun erkek arılardan elde edilen sperm örnekleri birleştirildi. Daha sonra birleştirilen semen, farklı konsantrasyonlarda L-karnitin içeren (2.5 mM/5 mM/10 mM) ve içermeyen (kontrol) sulandırıcılar ile seyreltildi. Motilite, plazma membranı fonksiyonel bütünlüğü, akrozomal bütünlük ve mitokondriyal fonksiyon kriyoprezervasyon sürecinden olumsuz etkilenmiş, ancak DNA bütünlüğü etkilenmemiştir. Malondialdehit (MDA) konsantrasyonu, membran lipid peroksidasyon durumunu analiz etmek için kullanıldı ve çözdürme sonrası kontrol grubuna kıyasla LC5 grubunda daha iyi bir sonuç elde edildi. L-karnitin grupları, kontrol grubuna kıyasla sperm motilitesi, plazma membran bütünlüğü ve mitokondriyal fonksiyon üzerinde olumlu bir etkiye sahipti (P<0.05). Akrozomal bütünlük, kontrol grubuna kıyasla LC5 grubunda daha iyi korunmuştur. Çalışma, LC takviyeli sulandırıcıların bal arısı sperm parametreleri üzerinde faydalı etkilere sahip olduğunu göstermektedir. Mevcut çalışmanın sonuçları, 5 mMol LC takviyeli sulandırıcı kullanmanın faydalı etkisini gösterdi.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Alçay S, Seven Çakmak S, Cakmak I, AKTAR A, Yilmaz M, ustuner b, Akkaşoğlu M, TAŞKIRAN Ş, Ayaz E, SAĞIRKAYA H, NUR Z (2021). L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa. , 489 - 493. 10.9775/kvfd.2021.25756 |
| Chicago | Alçay Selim,Seven Çakmak Selvinar,Cakmak Ibrahim,AKTAR Ahmet,Yilmaz Mehmet Melih,ustuner burcu,Akkaşoğlu Mustafa,TAŞKIRAN ŞEYMA,Ayaz Elif,SAĞIRKAYA Hakan,NUR ZEKARIYA L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa. (2021): 489 - 493. 10.9775/kvfd.2021.25756 |
| MLA | Alçay Selim,Seven Çakmak Selvinar,Cakmak Ibrahim,AKTAR Ahmet,Yilmaz Mehmet Melih,ustuner burcu,Akkaşoğlu Mustafa,TAŞKIRAN ŞEYMA,Ayaz Elif,SAĞIRKAYA Hakan,NUR ZEKARIYA L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa. , 2021, ss.489 - 493. 10.9775/kvfd.2021.25756 |
| AMA | Alçay S,Seven Çakmak S,Cakmak I,AKTAR A,Yilmaz M,ustuner b,Akkaşoğlu M,TAŞKIRAN Ş,Ayaz E,SAĞIRKAYA H,NUR Z L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa. . 2021; 489 - 493. 10.9775/kvfd.2021.25756 |
| Vancouver | Alçay S,Seven Çakmak S,Cakmak I,AKTAR A,Yilmaz M,ustuner b,Akkaşoğlu M,TAŞKIRAN Ş,Ayaz E,SAĞIRKAYA H,NUR Z L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa. . 2021; 489 - 493. 10.9775/kvfd.2021.25756 |
| IEEE | Alçay S,Seven Çakmak S,Cakmak I,AKTAR A,Yilmaz M,ustuner b,Akkaşoğlu M,TAŞKIRAN Ş,Ayaz E,SAĞIRKAYA H,NUR Z "L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa." , ss.489 - 493, 2021. 10.9775/kvfd.2021.25756 |
| ISNAD | Alçay, Selim vd. "L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa". (2021), 489-493. https://doi.org/10.9775/kvfd.2021.25756 |
| APA | Alçay S, Seven Çakmak S, Cakmak I, AKTAR A, Yilmaz M, ustuner b, Akkaşoğlu M, TAŞKIRAN Ş, Ayaz E, SAĞIRKAYA H, NUR Z (2021). L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27(4), 489 - 493. 10.9775/kvfd.2021.25756 |
| Chicago | Alçay Selim,Seven Çakmak Selvinar,Cakmak Ibrahim,AKTAR Ahmet,Yilmaz Mehmet Melih,ustuner burcu,Akkaşoğlu Mustafa,TAŞKIRAN ŞEYMA,Ayaz Elif,SAĞIRKAYA Hakan,NUR ZEKARIYA L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27, no.4 (2021): 489 - 493. 10.9775/kvfd.2021.25756 |
| MLA | Alçay Selim,Seven Çakmak Selvinar,Cakmak Ibrahim,AKTAR Ahmet,Yilmaz Mehmet Melih,ustuner burcu,Akkaşoğlu Mustafa,TAŞKIRAN ŞEYMA,Ayaz Elif,SAĞIRKAYA Hakan,NUR ZEKARIYA L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.27, no.4, 2021, ss.489 - 493. 10.9775/kvfd.2021.25756 |
| AMA | Alçay S,Seven Çakmak S,Cakmak I,AKTAR A,Yilmaz M,ustuner b,Akkaşoğlu M,TAŞKIRAN Ş,Ayaz E,SAĞIRKAYA H,NUR Z L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(4): 489 - 493. 10.9775/kvfd.2021.25756 |
| Vancouver | Alçay S,Seven Çakmak S,Cakmak I,AKTAR A,Yilmaz M,ustuner b,Akkaşoğlu M,TAŞKIRAN Ş,Ayaz E,SAĞIRKAYA H,NUR Z L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(4): 489 - 493. 10.9775/kvfd.2021.25756 |
| IEEE | Alçay S,Seven Çakmak S,Cakmak I,AKTAR A,Yilmaz M,ustuner b,Akkaşoğlu M,TAŞKIRAN Ş,Ayaz E,SAĞIRKAYA H,NUR Z "L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27, ss.489 - 493, 2021. 10.9775/kvfd.2021.25756 |
| ISNAD | Alçay, Selim vd. "L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27/4 (2021), 489-493. https://doi.org/10.9775/kvfd.2021.25756 |
