Objective: This study aims to compare the diagnostic effectiveness of fragment analysis and next-generationsequencing methods in Familial Mediterranean Fever (FMF) disease. Data from the study will guide clinicians in thediagnosis of FMF patients Method: Two hundred and ninety patients with normal (N/N) or heterozygous (M1/N) fragment analysis resultshad been included in the study in the medical genetics laboratory of Kayseri City Hospital. In these patients, theentire MEFV gene was examined by the NGS analysis method. The mutation profiles of these patients were com pared retrospectively. Statistical analysis of the data was performed using SPSS 22.0 package program. Results: All mutations found in the fragment analysis were also detected by NGS method. Additional mutationswere observed in 22 patients in the NGS analysis method. Mutations that were not previously reported in theHGMD, Clinvar and Infevers database were introduced into the literature. The application of NGS method to thepatients was found to be statistically significant (p<0.01) Conclusion: Fragment analysis is a low-cost method that examines the frequent changes in the diagnosis of FMFpatients but is insufficient to make a definitive diagnosis. NGS analysis method should be a preferable analysismethod, in terms of diagnodtic usefulness.