Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells
Yıl: 2021 Cilt: 9 Sayı: 4 Sayfa Aralığı: 203 - 806 Metin Dili: İngilizce DOI: 10.24925/turjaf.v9i4.803-806.4238 İndeks Tarihi: 27-11-2021
Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells
Öz: Licorice, also known as the root of Glycyrrhiza glabra, has been used for many years in traditional medicine to treat various diseases. Licorice root has remarkable pharmacological properties and these biological effects are predominantly attributed to its content of polyphenols and flavonoids. The aim of this study was to determine the proliferative effect of licorice root extract on human osteoblast cells. The study groups were exposed to various concentrations of licorice root extract on 31.25, 62.5, 250, 500, 1000 μg/mL for 24, and 48 h. The proliferative effect of the extract on human osteoblast cells was assessed using the MTT assay. After 24 and 48 h, cell proliferation of groups treated were increased statistically significant compared to the control cells, and also all concentrations showing no cytotoxic effects on osteoblast cells. Phytomedical applications of licorice root may represent a promising approach in the treatment of periodontal regeneration and osteoporosis.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Asl MN, Hosseinzadeh H. 2008. Review of pharmacological effects of Glycyrrhiza sp. and its bioactive compounds. Phytother Res, 22:709e24.
- Azizsoltani A, Piri K, Behzad S, Soleimani M, Nekouei M, Mahmoudi Z, Kazemi A. 2018. Ethyl Acetate Extract of Licorice Root (Glycyrrhiza glabra) Enhances Proliferation and Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells. Iran J Pharm Res, 17(3):1057- 1067.
- Cekici A, Kantarci A, Hasturk H, Dyke TEV. 2000. Inflammatory and immune pathways in the pathogenesis of periodontal disease. Periodontol, 64(1): 57-80.
- Che CT, Wong MS, Lam CW. 2016. Natural products from Chinese medicines with potential benefits to bone health. Molecules, 21(3): 239.
- Cho YE, Alcantara E, Kumaran S, Son KH, Sohn HY, Lee JH, Choi CS, Ha TY, Kwun IS. 2010. Red yeast rice stimulates osteoblast proliferation and increases alkaline phosphatase activity in MC3T3-E1 cells. Nutr Res, 30(7): 501-510.
- Cho YE, Kwun IS. 2018.Glycyrrhiza uralensis (licorice) extracts increase cell proliferation and bone markerenzyme alkaline phosphatase activity in osteoblastic MC3T3-E1 cells. Journal of Nutrition and Health, 51(3): 316.
- Choi EM. 2005. The licorice root derived isoflavan glabridin increases the function of osteoblastic MC3T3-E1 cells. Biochem. Pharmacol, 70: 363-368.
- Choi EM. 2011. Glabridin protects osteoblastic MC3T3-E1 cells against antimycin A induced cytotoxicity. Chem Biol Interact,193 (1): 71-78.
- Fatima A, Gupta VK, Luqman S, Arvind SN, Kumar JK, Shanker K, Saikia D, Srivastava S, Darokar MP, Khanuja SPS. 2009. Antifungal activity of Glycyrrhizia glabra extracts and its active constituent glabridin. Phytother Res, 23: 1190-1193.
- Fiore C, Eisenhut M, Ragazzi E, et al. 2005. A history of the therapeutic use of liquorice in Europe. J Ethnopharmacol, 99: 317-324.
- Frion-Herrera Y, Diaz-Garcia A, Rodriguez-Sanchez H, RuizFuentes J, Monzote-Fidalgo L, et al. 2013. Cytotoxic effect of Cuban propolis extracts against tumor cells lines. Am JEssen Oils Nat Prod, (1): 112-117,
- Garlet GP. 2010. Destructive and protective roles of cytokines in periodontitis: a re-appraisal from host defense and tissue destruction viewpoints. J Dent Res, 89: 1349-1363.
- He J, Chen L, Heber D, Shi W, Lu QY. 2006. Antibacterial compounds from Glycyrrhiza uralensis. J Nat Prod, 69: 121-124.
- Hosseinzadeh H, Nassiri-Asl M. 2015. Pharmacological effects of Glycyrrhiza spp. and its bioactive constituents: update and review. Phytother Res, 29(12):1868-1886.
- Hu CH, He J, Eckert R. et al. 2011. Development and evaluation of a safe and effective sugar-free herbal lollipop that kills cavity-causing bacteria. Int J Oral Sci, 3: 13-20.
- Huttner EA, Machado DC, Oliveira RB de, Antunes AGF, Hebling E. 2009. Effects of human aging on periodontal tissues. Spec Care Dentist, 29(4):149-55.
- Isbrucker RA, Burdock GA. 2006. Risk and safety assess-ment on the consumption of licorice root (Glycyrrhiza sp.), its extract and powder as a food ingredient, with emphasison the pharmacology and toxicology of glycyrrhizin. RegulToxicol Pharmacol, 26:167-192.
- Kaur P, Kaur S, Kumar N, Singh B, Kumar S. 2009. Evaluation of antigenotoxic activity of isoliquiritin apioside from Glycyrrhiza glabra L. Toxicol. In vitro: Int. J. Publ. Assoc. BIBRA 23: 680-686.
- Kim JH, Kim M, Jung HS, Sohn Y. 2019. Leonurus sibiricus L. ethanol extract promotes osteoblast differentiation and inhibits osteoclast formation. Int J Mol Med, 44(3):913-926.
- Kızıldağ A, Dalyanoğlu Mm, Yazkan B, Kara R. 2020. Effects of Different Concentrations of Crithmum Maritimum L. on Periodontal Ligament Fibroblast Cells. Turkiye Klinikleri J Dental Sci, 26(1): 31-35
- La VD, Tanabe S, Bergeron C, Gafner S, Grenier D (2011). Modulation of matrix metalloproteinase and cytokine production by licorice isolates licoricidin and licorisoflavan A: potential therapeutic approach for periodontitis. J Periodontol 82: 122–128.
- Malvania EA, Sharma AS, Sheth SA, Rathod S, Chovatia NR, Kachwala MS. 2019. In Vitro Analysis of Licorice (Glycyrrhiza glabra) Root Extract Activity on Streptococcus mutans in Comparison to Chlorhexidine and Fluoride Mouthwash. J Contemp Dent Pract, 20(12):1389-1394.
- Messier C, F Epifano, S Genovese, D Grenier. 2012. Licorice and its potential beneficial effects in common oro-dental diseases. Oral Dis, 18(1):32-9.
- Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 65: 55-63.
- Nassiri Asl M, Hosseinzadeh H. 2008. Review of pharmacological effects of Glycyrrhiza sp. and its bioactive compounds. Phytother Res, 22: 709-724.
- Nazari S, Rameshrad M, Hosseinzadeh H. 2017.Toxicological Effects of Glycyrrhiza glabra (Licorice): A Review. Phytother Res, 31(11):1635-1650.
- Oringer RJ. 2002. Biological mediators for periodontal and bone regeneration. Compendium, 23: 501-503.
- Park SY, Lim SS, Kim JK, Kang IJ, Kim JS, Lee C, Kim J, Park JH. 2010. Hexane-ethanol extract of Glycyrrhiza uralensis containing licoricidin inhibits the metastatic capacity of DU145 human prostate cancer cells. Br J Nutr, 104(9): 1272- 1282.
- Sasaki H, Suzuki N, AlShwaimi E. et al. 2010. 18bGlycyrrhetinic acid inhibits periodontitis via glucocorticoidindependent nuclear factor-jB inactivation in interleukin-10- deficient mice. J Periodontal Res, 45: 757-763.
- Seon MR, Park SY, Kwon SJ, Lim SS, Choi HJ, Park H, Lim DY, Kim JS, Lee CH, Kim J, Park JH. 2012. Hexane/ethanol extract of Glycyrrhiza uralensis and its active compound isoangustone A induce G1 cycle arrest in DU145 human prostate and 4T1 murine mammary cancer cells. J Nutr Biochem, 23(1): 85-92.
- Shanmugapriya K, Kim H, Kang HW. 2019. In vitro antitumor potential of astaxanthin nanoemulsion against cancer cells via mitochondrial mediated apoptosis. Int J Pharm, 560: 334-346.
- Shen XP, Xiao PG, Liu CX. 2007. Research and applicationof Radix Glycyrrhizae. Asian J Drug Metab Pharmacokinet, 7: 181-200.
- So derling E, Karjalainen S, Lille M, Maukonen J, Saarela M, Autio K. 2006. The effect of liquorice extract-containing starch gel on the amount and microbial composition of plaque. Clin Oral Invest, 10: 108-113.
- Vaya J, Belinky PA, Aviram M. 1997Antioxidant constituents from licorice roots: isolation, structure elucidation and antioxidativecapacity toward LDL oxidation. Free Radic Biol Med, 23(2): 302-313.
- Wang ZY, Nixon DW. 2001. Licorice and cancer. Nutr Cancer, 39(1): 1-11.
- Wang X, Zhang, Chen L, Shan L, Fan G, Gao X. 2013. Liquorice, a unique "guide drug" of traditional Chinese medicine: a review of its role in drug interactions. J Ethnopharmacol, 150(3):781-90.
| APA | Mısır S (2021). Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells. , 203 - 806. 10.24925/turjaf.v9i4.803-806.4238 |
| Chicago | Mısır Sema Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells. (2021): 203 - 806. 10.24925/turjaf.v9i4.803-806.4238 |
| MLA | Mısır Sema Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells. , 2021, ss.203 - 806. 10.24925/turjaf.v9i4.803-806.4238 |
| AMA | Mısır S Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells. . 2021; 203 - 806. 10.24925/turjaf.v9i4.803-806.4238 |
| Vancouver | Mısır S Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells. . 2021; 203 - 806. 10.24925/turjaf.v9i4.803-806.4238 |
| IEEE | Mısır S "Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells." , ss.203 - 806, 2021. 10.24925/turjaf.v9i4.803-806.4238 |
| ISNAD | Mısır, Sema. "Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells". (2021), 203-806. https://doi.org/10.24925/turjaf.v9i4.803-806.4238 |
| APA | Mısır S (2021). Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, 9(4), 203 - 806. 10.24925/turjaf.v9i4.803-806.4238 |
| Chicago | Mısır Sema Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells. Türk Tarım - Gıda Bilim ve Teknoloji dergisi 9, no.4 (2021): 203 - 806. 10.24925/turjaf.v9i4.803-806.4238 |
| MLA | Mısır Sema Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, vol.9, no.4, 2021, ss.203 - 806. 10.24925/turjaf.v9i4.803-806.4238 |
| AMA | Mısır S Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells. Türk Tarım - Gıda Bilim ve Teknoloji dergisi. 2021; 9(4): 203 - 806. 10.24925/turjaf.v9i4.803-806.4238 |
| Vancouver | Mısır S Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells. Türk Tarım - Gıda Bilim ve Teknoloji dergisi. 2021; 9(4): 203 - 806. 10.24925/turjaf.v9i4.803-806.4238 |
| IEEE | Mısır S "Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells." Türk Tarım - Gıda Bilim ve Teknoloji dergisi, 9, ss.203 - 806, 2021. 10.24925/turjaf.v9i4.803-806.4238 |
| ISNAD | Mısır, Sema. "Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells". Türk Tarım - Gıda Bilim ve Teknoloji dergisi 9/4 (2021), 203-806. https://doi.org/10.24925/turjaf.v9i4.803-806.4238 |
