The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice

KAHRAMAN, AHMET; KÖKEN, Tülay; Serteser, Mustafa; Akbulut, Gökhan; Dilek, Osman Nuri

The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice

Gökhan AKBULUT, (Kocatepe Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, Afyon, Türkiye)

Osman Nuri DİLEK, (Kocatepe Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, Afyon, Türkiye)

Ahmet KAHRAMAN, (Afyon Kocatepe Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Afyon, Türkiye)

Tülay KÖKEN, (Afyon Kocatepe Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Afyon, Türkiye)

Mustafa SERTESER (Afyon Kocatepe Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Afyon, Türkiye)

Ulusal Travma Dergisi

2 0

Yıl: 2005 Cilt: 11 Sayı: 1 Sayfa Aralığı: 11 - 16 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice

Öz:

AMAÇ Amacımız farklı iskemi ve ardından uygulanan reperfüzyon sürelerinde oluşan böbrek I/R modelinde oksidatif stres ile pro-inflamatuar yanıt aras›ndaki ilişkiyi araştırmaktı. GEREÇ VE YÖNTEM Yirmi dört erkek İsviçre fındık faresi 30,45,60 dakikalık sürelerde iskemi ve ard›ndan 60 dakikalk reperfüzyona maruz bırakılmıştır (sırasıyla Grup 1, I30/R60; Grup 2, I45/R60;Grup3, I 60/R60). Oksidatif stres ve antioksidan aktivite belirteci olarak böbrek dokusunda tiobarbitürik tiyobarbitürik asit reaktif madde (TBARS), protein karbonil ve sülfidril (SH),doku süperoksit dismutaz› (SOD) ve katalaz (CAT) aktivitesi çalışıldı.İskemi / reperfüzyona pro - inflamatuar yanıt olarak böbrek dokusu TNF-a düzeyleri araştırıldı. BULGULAR Oksidatif stres ve antioksidan aktivite belirteci olan TBARS ve protein karbonil düzeyleri I30/R60 grubunda artmıştır. (p=0,01). I45/R60 grubunda ise TBARS ve protein karbonil ve TNF-a düzeyleri anlamlı derecede artarken SOD ve CAT (p=0,01) aktivitesi ve SH düzeylerinin anlamlı olarak azalmıştır.(p=0,05). Bu bulgular I 60/R60 grubunda daha belirgindi. TNF-α düzeyindeki artışın oksidatif stres belirteçleri olan TBARS ve protein karbonil düzeyleri ile pozitif ve antioksidan aktivite belirteçleri olan SOD, CAT ve renal doku SH düzeyleri ile negatif ilişkisi olduğu belirlendi (p=0,001). SONUÇ Çalışmamızda fındık farelerinde 45 dakikalık iskemi ve ardından 60 dakikalık reperfüzyonun pro-inflamatuar süreçler zincirini TNF-α üzerinden indükleyebildiği sonucuna vardık.

Anahtar Kelime: Tümör nekrozis faktör-alfa Tiyobarbitürik asit reaktif maddeleri Oksidatif stres Böbrek Myoxidae Reperfüzyon hasarı

Sıçanlarda iskemi-reperfüsyon modelinde böbrek dokusundaki oksidatif stres parametreleri ve TNF-alfa seviyelerinin ilişkisi

Öz:

BACKGROUND: To investigate the relationship between oxidative stress and pro-inflammatory response of the renal tissue at different ischemia times in I/R mice.METHODS:Twenty-four male Swiss dormice were subjected to 30, 45 or 60 minutes of ischemia and then 60 minutes of reperfusion (Group 1 I 30/R60, Group 2, .I45/R60, and Group 3, I 60/R60 respectively). As markers of oxidative stress and antioxidant activity, levels of thiobarbituric acid reactive substances (TBARS), protein carbonyls and protein sulfhydryls (SH), tissue superoxide dismutase (SOD) and catalase (CAT) activities of the renal tissue and also renal tissue pro-inflammatory marker TNF-alpha levels were assessed.RESULTS: The levels of TBARS and protein carbonyl rised in I30/R60 group (p=0.01). In I45/R60 group, levels of TBARS, protein carbonyls and TNF-alpha levels were significantly higher while SOD and CAT activities (p=0.01), and the levels of SH were significantly decreased (p=0.05). These findings were more relevant for I60/R60 group. Higher TNF-alpha levels correlated positively with higher levels of TBARS, and protein carbonyls and negatively with SOD, CAT and renal tissue SH (p=0.001).CONCLUSIONS: In mice, oxidative stress after 45 minutes ischemia and 60 min reperfusion could induce pro-inflammatory cascade mediated through TNF-alpha.

Anahtar Kelime: Myoxidae Reperfusion Injury Tumor Necrosis Factor-alpha Thiobarbituric Acid Reactive Substances Oxidative Stress Kidney

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Akbulut G, Dilek O, KAHRAMAN A, KÖKEN T, Serteser M (2005). The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice. , 11 - 16.
Chicago	Akbulut Gökhan,Dilek Osman Nuri,KAHRAMAN AHMET,KÖKEN Tülay,Serteser Mustafa The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice. (2005): 11 - 16.
MLA	Akbulut Gökhan,Dilek Osman Nuri,KAHRAMAN AHMET,KÖKEN Tülay,Serteser Mustafa The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice. , 2005, ss.11 - 16.
AMA	Akbulut G,Dilek O,KAHRAMAN A,KÖKEN T,Serteser M The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice. . 2005; 11 - 16.
Vancouver	Akbulut G,Dilek O,KAHRAMAN A,KÖKEN T,Serteser M The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice. . 2005; 11 - 16.
IEEE	Akbulut G,Dilek O,KAHRAMAN A,KÖKEN T,Serteser M "The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice." , ss.11 - 16, 2005.
ISNAD	Akbulut, Gökhan vd. "The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice". (2005), 11-16.

APA	Akbulut G, Dilek O, KAHRAMAN A, KÖKEN T, Serteser M (2005). The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice. Ulusal Travma Dergisi, 11(1), 11 - 16.
Chicago	Akbulut Gökhan,Dilek Osman Nuri,KAHRAMAN AHMET,KÖKEN Tülay,Serteser Mustafa The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice. Ulusal Travma Dergisi 11, no.1 (2005): 11 - 16.
MLA	Akbulut Gökhan,Dilek Osman Nuri,KAHRAMAN AHMET,KÖKEN Tülay,Serteser Mustafa The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice. Ulusal Travma Dergisi, vol.11, no.1, 2005, ss.11 - 16.
AMA	Akbulut G,Dilek O,KAHRAMAN A,KÖKEN T,Serteser M The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice. Ulusal Travma Dergisi. 2005; 11(1): 11 - 16.
Vancouver	Akbulut G,Dilek O,KAHRAMAN A,KÖKEN T,Serteser M The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice. Ulusal Travma Dergisi. 2005; 11(1): 11 - 16.
IEEE	Akbulut G,Dilek O,KAHRAMAN A,KÖKEN T,Serteser M "The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice." Ulusal Travma Dergisi, 11, ss.11 - 16, 2005.
ISNAD	Akbulut, Gökhan vd. "The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice". Ulusal Travma Dergisi 11/1 (2005), 11-16.