Objective: The side- effects of antibacterial agents thatwere used for the removal of caries causing microorganisms from the oralenvironment to prevent dental caries have increased the interests of peo ple towards complementary and alternative treatments for their healthneeds. The aim of this study is to analyze the effect of black carrot, cherryand pomegranate concentrates, which can be harvested in Turkey, onbiofilm forming properties of Streptococcus mutans. Material and Meth ods: Black carrot, cherry and pomegranate concentrates (100%) and S.mutans ATCC 25175 origins were used. In order to control the antibacte rial activity of black carrot, cherry and pomegranate concentrates, mini mal inhibitory concentration values were determined. Crystal violetstaining method was used to control their effects on biofilm forming ac tivity. Bacterial suspensions were prepared by using the Triptych broth(Oxoid) and prepared to be 0.5 McFarland turbidity standard (1.5x108CFU/ml) at OD (Optical Density) 600 nm. Results: It was observed thatblack carrot, cherry and pomegranate concentrates did not have an an tibacterial effect on growth of S. mutans. When the effect of concentrateson biofilm forming property of S. mutans was examined, it was observedthat there was a statistically significant difference (p<0.05) between con centrates and negative control group. It was observed in the dual exami nations that there is a statistically significant difference between negativecontrol group and pomegranate concentrate after 24 hours and cherry con centrate after 48 hours (p<0.008). No statistically significant differencewas observed between black carrot concentrate and negative control groupuntil 96 hours. (p>0.008). Conclusion: Black carrot concentrate did notinhibit bacterial increase; on the other hand, it supressed the biofilm for mation until 96 hours. Considering the biofilm formation disrupting effectsof black carrot which is abundantly grown in Turkey, its use as an alter native active ingredient in products such as chewing gum and pastille,which are frequently consumed by children, should be further investigatedin future studies.