Yıl: 2021 Cilt: 5 Sayı: 2 Sayfa Aralığı: 112 - 119 Metin Dili: İngilizce DOI: 10.30616/ajb.959040 İndeks Tarihi: 29-07-2022

Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria

Öz:
This study aimed to identify the chemical composition (ash, cellulose, total chlorophylls, carotenoid, and essential oil (EO) content), antioxidant activity, and mineral composition of the lemon balm (Melissa officinalis L) obtained from Bulgaria. The chemical composition of the EO was analyzed using gas chromatography-mass spectrometry (GC-MS). The major constituents of the EO were caryophyllene oxide (33.99%), n-hexadecanoic acid (14.28%), α-citral (6.62%), (5E,9E)-farnesyl acetone (5.00%), β-caryophyllene (4.82%), (2E,6Z)-farnesal (4.56%), (5E,9Z)-farnesyl acetone (4.16%), β-citral (3.98%), and γ-eudesmol (3.13%). The antioxidant potential was assessed using DPPH (1,1-diphenyl-2-picrilhydrazyl) free radical, ferric reducing antioxidant power assay (FRAP), TEAC assay, determination of hydroxyl radical scavenging capacity and determination of superoxide scavenging capacity methods. The total phenol content was analyzed using the Folin-Ciocalteu method. The total flavonoid amount of the extracts was determined by optimizing the aluminum chloride colorimetric method. The total phenolic content, the total flavonoid composition, and the antioxidant potential of the lemon balm leaves included 184.33 mg GAE g-1, 12.65 mg QE g-1, and 62.83 μg ml-1, respectively. Mineral and heavy metal contents were determined by the iCAP-Qc ICP-MS spectrometer. The highest amounts of macro and microminerals were determined for K, Ca, Mg, Sr, Rb, Ba, Mn, and Fe, respectively.
Anahtar Kelime: ICP-MS antioxidant activity chemical composition GS-MS Melissa officinalis L.

Bulgaristan florasından toplanan yabani melisa (Melissa officinalis L.)’nın fitokimyasal bileşimi

Öz:
Bu çalışma ile Bulgaristan florasından toplanan melisanın (Melissa officinalis L) kimyasal bileşenleri (kül, selüloz, toplam klorofil, karotenoid ve uçucu yağ (EO) içeriği), antioksidan aktivitesi ve mineral madde bileşenlerinin belirlenmesi amaçlanmıştır. Uçucu yağ analizinde gaz kromatografisi-kütle spektrometrisi (GC-MS) cihazı kullanılmıştır. Uçucu yağın ana bileşenleri, karyofilen oksit (%33.99), n-heksadekanoik asit (%14.28), α-sitral (%6.62), (5E,9E)-farnesil aseton (%5.00), β-karyofilen (%4.82), (2E,6Z)-farnesal (%4.56), (5E,9Z)-farnesil aseton (%4,16), β-sitral (%3,98) ve γ-eudesmol (%3.13) olarak tespit edilmiştir. Antioksidan kapasitesini belirlemek için DPPH (1,1-difenil-2-pikrilhidrazil) serbest radikali, ferrik indirgeyici antioksidan güç testi (FRAP), TEAC testi, hidroksil radikali süpürme kapasitesi ve süperoksit süpürme kapasitesi yöntemleri kullanılmıştır. Toplam fenol içeriği, Folin-Ciocalteu yöntemi kullanılarak analiz edilmiştir. Ekstraktların toplam flavonoid miktarı, alüminyum klorür kolorimetrik yöntemi optimize edilerek belirlenmiştir. Melisa yapraklarının toplam fenolik içeriği, toplam flavonoid içeriği ve antioksidan kapasitesi sırasıyla 184.33 mg GAE g-1, 12.65 mg QE g-1 ve 62.83 μg ml-1 olarak tespit edilmiştir. Mineral madde ve ağır metal içerikleri, iCAP-Qc ICP-MS spektrometresi ile belirlenmiştir. En yüksek makro ve mikromineral miktarı sırasıyla K, Ca, Mg, Sr, Rb, Ba, Mn ve Fe olarak belirlenmiştir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Doğan H, Uskutoğlu T, baş h, Stankov S, Fidan H, COŞGE ŞENKAL B, Stoyanova A, Petkova N, YILMAZ G, Dincheva I (2021). Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria. , 112 - 119. 10.30616/ajb.959040
Chicago Doğan Hülya,Uskutoğlu Tansu,baş hatice,Stankov Stanko,Fidan Hafize,COŞGE ŞENKAL Belgin,Stoyanova Albena,Petkova Nadezhda,YILMAZ GÜNGÖR,Dincheva Ivayla Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria. (2021): 112 - 119. 10.30616/ajb.959040
MLA Doğan Hülya,Uskutoğlu Tansu,baş hatice,Stankov Stanko,Fidan Hafize,COŞGE ŞENKAL Belgin,Stoyanova Albena,Petkova Nadezhda,YILMAZ GÜNGÖR,Dincheva Ivayla Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria. , 2021, ss.112 - 119. 10.30616/ajb.959040
AMA Doğan H,Uskutoğlu T,baş h,Stankov S,Fidan H,COŞGE ŞENKAL B,Stoyanova A,Petkova N,YILMAZ G,Dincheva I Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria. . 2021; 112 - 119. 10.30616/ajb.959040
Vancouver Doğan H,Uskutoğlu T,baş h,Stankov S,Fidan H,COŞGE ŞENKAL B,Stoyanova A,Petkova N,YILMAZ G,Dincheva I Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria. . 2021; 112 - 119. 10.30616/ajb.959040
IEEE Doğan H,Uskutoğlu T,baş h,Stankov S,Fidan H,COŞGE ŞENKAL B,Stoyanova A,Petkova N,YILMAZ G,Dincheva I "Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria." , ss.112 - 119, 2021. 10.30616/ajb.959040
ISNAD Doğan, Hülya vd. "Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria". (2021), 112-119. https://doi.org/10.30616/ajb.959040
APA Doğan H, Uskutoğlu T, baş h, Stankov S, Fidan H, COŞGE ŞENKAL B, Stoyanova A, Petkova N, YILMAZ G, Dincheva I (2021). Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria. Anatolian Journal of Botany, 5(2), 112 - 119. 10.30616/ajb.959040
Chicago Doğan Hülya,Uskutoğlu Tansu,baş hatice,Stankov Stanko,Fidan Hafize,COŞGE ŞENKAL Belgin,Stoyanova Albena,Petkova Nadezhda,YILMAZ GÜNGÖR,Dincheva Ivayla Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria. Anatolian Journal of Botany 5, no.2 (2021): 112 - 119. 10.30616/ajb.959040
MLA Doğan Hülya,Uskutoğlu Tansu,baş hatice,Stankov Stanko,Fidan Hafize,COŞGE ŞENKAL Belgin,Stoyanova Albena,Petkova Nadezhda,YILMAZ GÜNGÖR,Dincheva Ivayla Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria. Anatolian Journal of Botany, vol.5, no.2, 2021, ss.112 - 119. 10.30616/ajb.959040
AMA Doğan H,Uskutoğlu T,baş h,Stankov S,Fidan H,COŞGE ŞENKAL B,Stoyanova A,Petkova N,YILMAZ G,Dincheva I Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria. Anatolian Journal of Botany. 2021; 5(2): 112 - 119. 10.30616/ajb.959040
Vancouver Doğan H,Uskutoğlu T,baş h,Stankov S,Fidan H,COŞGE ŞENKAL B,Stoyanova A,Petkova N,YILMAZ G,Dincheva I Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria. Anatolian Journal of Botany. 2021; 5(2): 112 - 119. 10.30616/ajb.959040
IEEE Doğan H,Uskutoğlu T,baş h,Stankov S,Fidan H,COŞGE ŞENKAL B,Stoyanova A,Petkova N,YILMAZ G,Dincheva I "Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria." Anatolian Journal of Botany, 5, ss.112 - 119, 2021. 10.30616/ajb.959040
ISNAD Doğan, Hülya vd. "Phytochemical composition of wild lemon balm (Melissa officinalis L.) from the flora of Bulgaria". Anatolian Journal of Botany 5/2 (2021), 112-119. https://doi.org/10.30616/ajb.959040