Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model

Ustundag, Unsal Veli; Yarat, Aysen; Tunali-Akbay, Tugba; Özçelik, Reyhan; Alev-Tuzuner, Burcin; Şener, Göksel; Emekli-Alturfan, Ebru; HAKGÜDER, Umay; İpekçi, Hazal; Kahraman, Nevin Genc

doi:10.26650/experimed.2021.899430

Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model

Umay HAKGÜDER, (Marmara Üniversitesi, Diş Hekimliği Fakültesi, Biyokimya Bölümü, İstanbul, Türkiye)

Hazal İPEKÇİ, (Marmara Üniversitesi, Diş Hekimliği Fakültesi, Biyokimya Bölümü, İstanbul, Türkiye)

Tuğba TUNALI AKBAY, (Marmara Üniversitesi, Diş Hekimliği Fakültesi, Biyokimya Bölümü, İstanbul, Türkiye)

Ayşen YARAT, (Marmara Üniversitesi, Diş Hekimliği Fakültesi, Biyokimya Bölümü, İstanbul, Türkiye)

Ebru EMEKLİ ALTURFAN, (Marmara Üniversitesi, Diş Hekimliği Fakültesi, Biyokimya Bölümü, İstanbul, Türkiye)

Ünsal Veli ÜSTÜNDAĞ, (İstanbul Medipol Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, İstanbul, Türkiye)

Burçin ALEV, (Gelişim Üniversitesi, Diş Hekimliği Fakültesi, Biyokimya Bölümü, İstanbul, Türkiye)

Nevin GENC KAHRAMAN, (İstanbul Medeniyet Üniversitesi, Göztepe Eğitim ve Araştırma Hastanesi, Tıbbi Biyokimya Anabilim Dalı, İstanbul, Türkiye)

Reyhan ÖZÇELİK, (Marmara Üniversitesi, Eczacılık Fakültesi, Farmakoloji Anabilim Dalı, İstanbul, Türkiye)

Göksel ŞENER (Fenerbahçe Üniversitesi, Eczacılık Fakültesi, Farmakoloji Anabilim Dalı, İstanbul, Türkiye)

EXPERIMED

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Yıl: 2021 Cilt: 11 Sayı: 1 Sayfa Aralığı: 21 - 26 Metin Dili: İngilizce DOI: 10.26650/experimed.2021.899430 İndeks Tarihi: 03-12-2021

Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model

Öz:

The aim of our study was to examine the effects of exercise and calorie restriction on various tissue damage and antioxidant parameters in the brain and testis of rats in a natural aging model. For this purpose, male Sprague-Dawley rats were the control group (C), the elderly (A), the elderly with calorie restriction (ACR), the elderly who were exercised (AE) and the elderly who were exercised with calorie restriction (ACRE), they were divided into 5 groups. The control group was composed of three-month-old animals. The other group consisted of 15-month-old rats. Exercise and calorie restriction were applied for 6 weeks. At the end of the experiment, lipid peroxidation (LPO), nitric oxide (NO), glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST) and tissue factor (TF) were determined in brain and testicular tissues homogenates. As a result of the study, the A group’s brain and testis LPO, NO levels and TF activity increased, GSH levels and SOD, CAT and GST activities decreased, when compared to the C group. As a result of our study, an increase in oxidant damage was observed with TF activity in the brain and testis in the natural aging model, and positive effects of exercise and calorie restriction on the antioxidant levels in the brain were determined, especially in aging.

Anahtar Kelime:

Doğal Yaşlanma Modelinde Egzersiz ve Kalori Kısıtlamasının Beyin ve Testiste Etkileri

Öz:

Amacımız doğal yaşlanma modelinde egzersiz ve kalori kısıtlamasının sıçanlarda beyin ve testiste çeşitli doku hasarı ve antioksidan parametreler üzerine etkilerinin incelenmesidir. Bu amaçla Sprague- Dawley sıçanlar kontrol (K), yaşlı (Y), kalori kısıtlaması uygulanan yaşlı (YKK), egzersiz uygulanan yaşlı (YE) ve kalori kısıtlaması ile egzersiz uygulanan yaşlı (YKKE) olmak üzere 5 gruba ayrıldı. Kontrol grubu üç aylık genç hayvanlardan oluşturuldu. Diğer gruplar 15 aylık sıçanlardan oluşturuldu. Egzersiz ve kalori kısıtlaması 6 hafta boyunca uygulandı. Deney sonunda beyin ve testis dokuları homojenatlarında lipit peroksidasyon (LPO), nitrik oksit (NO), glutatyon (GSH) düzeyleri ile süperoksit dismutaz (SOD), katalaz (CAT), glutatyon-S-transferaz (GST) ve doku faktörü (DF) aktiviteleri tayin edildi. Çalışma sonucunda K grubu ile kıyaslandığında Y grubunda, beyin ve testis LPO, NO düzeyleri ve DF aktivitelerinin arttığı, GSH düzeyleri ile SOD, CAT ve GST aktivitelerinin ise azaldığı gözlendi. Doğal yaşlanma modelinde beyin ve testiste DF aktivitesi ile beraber oksidan hasarda artış gözlenmiş olup, özellikle yaşlanmada egzersiz ve kalori kısıtlamasının beyinde antioksidan düzeyleri üzerinde olumlu etkileri belirlenmiştir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	HAKGÜDER U, İpekçi H, Tunali-Akbay T, Yarat A, Emekli-Alturfan E, Ustundag U, Alev-Tuzuner B, Kahraman N, Özçelik R, Şener G (2021). Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model. , 21 - 26. 10.26650/experimed.2021.899430
Chicago	HAKGÜDER Umay,İpekçi Hazal,Tunali-Akbay Tugba,Yarat Aysen,Emekli-Alturfan Ebru,Ustundag Unsal Veli,Alev-Tuzuner Burcin,Kahraman Nevin Genc,Özçelik Reyhan,Şener Göksel Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model. (2021): 21 - 26. 10.26650/experimed.2021.899430
MLA	HAKGÜDER Umay,İpekçi Hazal,Tunali-Akbay Tugba,Yarat Aysen,Emekli-Alturfan Ebru,Ustundag Unsal Veli,Alev-Tuzuner Burcin,Kahraman Nevin Genc,Özçelik Reyhan,Şener Göksel Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model. , 2021, ss.21 - 26. 10.26650/experimed.2021.899430
AMA	HAKGÜDER U,İpekçi H,Tunali-Akbay T,Yarat A,Emekli-Alturfan E,Ustundag U,Alev-Tuzuner B,Kahraman N,Özçelik R,Şener G Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model. . 2021; 21 - 26. 10.26650/experimed.2021.899430
Vancouver	HAKGÜDER U,İpekçi H,Tunali-Akbay T,Yarat A,Emekli-Alturfan E,Ustundag U,Alev-Tuzuner B,Kahraman N,Özçelik R,Şener G Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model. . 2021; 21 - 26. 10.26650/experimed.2021.899430
IEEE	HAKGÜDER U,İpekçi H,Tunali-Akbay T,Yarat A,Emekli-Alturfan E,Ustundag U,Alev-Tuzuner B,Kahraman N,Özçelik R,Şener G "Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model." , ss.21 - 26, 2021. 10.26650/experimed.2021.899430
ISNAD	HAKGÜDER, Umay vd. "Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model". (2021), 21-26. https://doi.org/10.26650/experimed.2021.899430

APA	HAKGÜDER U, İpekçi H, Tunali-Akbay T, Yarat A, Emekli-Alturfan E, Ustundag U, Alev-Tuzuner B, Kahraman N, Özçelik R, Şener G (2021). Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model. EXPERIMED, 11(1), 21 - 26. 10.26650/experimed.2021.899430
Chicago	HAKGÜDER Umay,İpekçi Hazal,Tunali-Akbay Tugba,Yarat Aysen,Emekli-Alturfan Ebru,Ustundag Unsal Veli,Alev-Tuzuner Burcin,Kahraman Nevin Genc,Özçelik Reyhan,Şener Göksel Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model. EXPERIMED 11, no.1 (2021): 21 - 26. 10.26650/experimed.2021.899430
MLA	HAKGÜDER Umay,İpekçi Hazal,Tunali-Akbay Tugba,Yarat Aysen,Emekli-Alturfan Ebru,Ustundag Unsal Veli,Alev-Tuzuner Burcin,Kahraman Nevin Genc,Özçelik Reyhan,Şener Göksel Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model. EXPERIMED, vol.11, no.1, 2021, ss.21 - 26. 10.26650/experimed.2021.899430
AMA	HAKGÜDER U,İpekçi H,Tunali-Akbay T,Yarat A,Emekli-Alturfan E,Ustundag U,Alev-Tuzuner B,Kahraman N,Özçelik R,Şener G Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model. EXPERIMED. 2021; 11(1): 21 - 26. 10.26650/experimed.2021.899430
Vancouver	HAKGÜDER U,İpekçi H,Tunali-Akbay T,Yarat A,Emekli-Alturfan E,Ustundag U,Alev-Tuzuner B,Kahraman N,Özçelik R,Şener G Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model. EXPERIMED. 2021; 11(1): 21 - 26. 10.26650/experimed.2021.899430
IEEE	HAKGÜDER U,İpekçi H,Tunali-Akbay T,Yarat A,Emekli-Alturfan E,Ustundag U,Alev-Tuzuner B,Kahraman N,Özçelik R,Şener G "Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model." EXPERIMED, 11, ss.21 - 26, 2021. 10.26650/experimed.2021.899430
ISNAD	HAKGÜDER, Umay vd. "Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model". EXPERIMED 11/1 (2021), 21-26. https://doi.org/10.26650/experimed.2021.899430