OBJECTIVE: To determine the microbiological agents in women with vaginal discharge.STUDY DESING: Sexually active 328 women with complaints of vaginal discharge were recruited into study. After direct microscopic examination and gram staining, vaginal and endocervical samples were inoculated into blood agar, eosin methylene blue, chocolate agar and Thayer Martin mediums. Chylamydia trachomatis antigen was detected by optic immunoassay method. Mycofast® 40 kit was used to detect Ureoplasma urealyticum and Mycoplasma hominis.RESULT(s): Normal vaginal flora was detected in % 9.14 (n=30) patients. Patogens isolated were in the following order: Ureoplasma urealyticum % 77.74 (n=255), Candida albicans % 21.03 (n=69), Ureoplasma urealyticum+Mycoplasma hominis % 18.29 (n=60), Gardnerella vaginalis % 16.46 (n=54), Trichomonas vaginalis % 8.53 (n=28), Chlamydia trachomatis % 1.82 (n=6), Neisseria gonorrhoeae % 0.06 (n=2).CONCLUSION: The main cause of vaginal discharge is the lower genital tractus. A great portion of these infections are polymicrobiol. Pathogens causing cervicitis are as much as responsible for the vaginal discharge as the microoganisms causing vaginitis.