Aim: In our study, it was aimed to determine the relationship between the use of personal protective equipment by healthcare workers in the COVID-19 epidemic and their depression, anxiety and stress levels. Material and Methods: 384 healthcare professionals working in a pandemic hospital participated in our descriptive study conducted in May 2020. The data collection tools; A form consisting of descriptive features, personal protective equipment use cases and Depression-Anxiety-Stress Scale (DASS-21) was used. The forms were delivered to healthcare professionals online and the responses were collected and analyzed in this way. Results: In our study, the Anxiety and Stress scores of female healthcare workers and the “DASS-21 anxiety score” of single healthcare workers were found to be statistically significantly higher (p=0.008; p=0.001; p=0.019; p<0.05). According to the professions, the scores of the healthcare workers on the DASS-21 scale did not show a statistically significant difference (p>0.05). During COVID-19 pandemic, statistically significant differences were found between Depression Anxiety and Stress scores of healthcare workers according to their visor or goggles / protective glasses use(p<0.01; p<0.05; p<0.01). Similarly, the use of disposable gowns / overalls and similar equipment during the epidemic caused a statistically significant difference in “DASS-21 depression and stress scores” in healthcare workers (p=0.002; p<0.01; p=0.029; p<0.05). Conclusion: It was observed that depression, anxiety and stress conditions of healthcare workersworking intensively during the COVID-19 outbreak were affected. Suitable working environments and necessary protective equipment should be provided for healthcare professionals. With the planning for the development of mental health protection programs, it can be ensured that they overcome the intensive process with the least damage.