The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo

ARAS, Hisar Şükriye; HİSAR, Olcay; Gulcin, ilhami; KUFREVIOGLU, Omer Irfan; BEYDEMİR, Şükrü; YANIK, PROF. DR.

The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo

Olcay HİSAR, (Atatürk Üniversitesi, Ziraat Fakültesi, Erzurum, Türkiye)

Şükrü BEYDEMİR, (Atatürk Üniversitesi, Fen Edebiyat Fakültesi, Erzurum, Türkiye)

İlhami GÜLÇİN, (Atatürk Üniversitesi, Fen Edebiyat Fakültesi, Erzurum, Türkiye)

Hisar Şükriye ARAS, (Atatürk Üniversitesi, Ziraat Fakültesi, Erzurum, Türkiye)

Telat YANIK, (Atatürk Üniversitesi, Ziraat Fakültesi, Erzurum, Türkiye)

Ömer İrfan KÜFREVİOĞLU (Atatürk Üniversitesi, Fen Edebiyat Fakültesi, Erzurum, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

1 0

Yıl: 2005 Cilt: 29 Sayı: 3 Sayfa Aralığı: 841 - 845 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo

Öz:

Bu çalışmada, Sepharose 4B-L tirozin-sülfanilamid afinite jel kromatografisi ile saflaştırılan gökkuşağı alabalığı {Oncorhynchus mykiss) eritrositlerindeki karbonik anhidraz enzimi üzerine melatonin hormonunun etkileri araştırılmıştır. CA enzimi Oncorhynchus mykiss eritrositlerinden -20 verim ile -200 kat saflaştırılmıştır. Enzimin spesifik aktivitesi ise - 400 EU/mg protein olarak hesaplanmıştır. İn vitro çalışmalarda melatonin hormonunun karbonik anhidraz enzimini inhibe etmediği tespit edilmiştir. In vivo çalışmalarda, kontrol grubu balıklarda karbonik anhidraz enzim aktivitesi 1597,7 ± 429,0 EU/g hemoglobin; melatonin enjeksiyonundan sonra 1., 3. ve 5. saatlerdeki karbonik anhidraz aktiviteleri ise sırasıyla 1406,4 ± 372,1,1346,3 ± 477,4 ve 783,8 ± 494,3 EU/g hemoglobin olarak belirlenmiştir. Oncorhynchus mykiss eritrosit karbonik anhidraz enziminin melatonin hormonu tarafından enjeksiyonu takiben 5. saatte önemli seviyede (P < 0,05) inhibe edildiği tespit edilmiştir. Çalışmadan elde edilen veriler göz önüne alındığında melatonin hormonunun üreme dönemindeki balıklarda aşırı miktarlarda kullanılmaması gerektiği önerilebilir.

Anahtar Kelime: karbonat dehidrataz enzimler Oncorhynchus mykiss gökkuşağı alabalığı hemoglobin melatonin enzim aktivitesi enzim inhibitörleri alyuvarlar

Konular: Ziraat Mühendisliği

Melatonin hormonunun Gökkuşağı Alabalığındaki (Oncorhynchus mykiss) eritrosit karbonik anhidraz enzimi üzerine in vitro ve in vivo etkileri

Öz:

The effects of melatonin hormone on rainbow trout (Oncorhynchus mykiss) erythrocyte carbonic anhydrase enzyme purified by using Sepharose 4B-L tyrosine-sulfanylamide affinity gel chromatography were investigated in vitro and in vivo. Carbonic anhydrase from Oncorhynchus mykiss erythrocytes was purified with a specific activity of -400 EU/mg protein and ~200-fold in a yield of -20. Melatonin did not inhibit carbonic anhydrase enzyme in vitro. Carbonic anhydrase activity of the control group was determined as 1597.7 ± 429.0 EU/g hemoglobin in vivo. Carbonic anhydrase activities were measured at 1, 3 and 5 h by following melatonin injection. The corresponding mean activities of melatonin were 1406.4 ± 372.1, 1346.3 ± 477.4 and 783.8 ± 494.3 EU/g hemoglobin respectively. Oncorhynchus mykiss erythrocyte carbonic anhydrase was inhibited significantly by melatonin hormone 5 h after injection in vivo (P < 0.05). Data from the present study suggest that excessive usage of this hormone should be avoided in fish reproduction.

Anahtar Kelime: rainbow trout haemoglobin melatonin enzyme activity enzyme inhibitors erythrocytes carbonate dehydratase enzymes Oncorhynchus mykiss

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	HİSAR O, BEYDEMİR Ş, Gulcin i, ARAS H, YANIK P, KUFREVIOGLU O (2005). The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo. , 841 - 845.
Chicago	HİSAR Olcay,BEYDEMİR Şükrü,Gulcin ilhami,ARAS Hisar Şükriye,YANIK PROF. DR.,KUFREVIOGLU Omer Irfan The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo. (2005): 841 - 845.
MLA	HİSAR Olcay,BEYDEMİR Şükrü,Gulcin ilhami,ARAS Hisar Şükriye,YANIK PROF. DR.,KUFREVIOGLU Omer Irfan The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo. , 2005, ss.841 - 845.
AMA	HİSAR O,BEYDEMİR Ş,Gulcin i,ARAS H,YANIK P,KUFREVIOGLU O The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo. . 2005; 841 - 845.
Vancouver	HİSAR O,BEYDEMİR Ş,Gulcin i,ARAS H,YANIK P,KUFREVIOGLU O The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo. . 2005; 841 - 845.
IEEE	HİSAR O,BEYDEMİR Ş,Gulcin i,ARAS H,YANIK P,KUFREVIOGLU O "The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo." , ss.841 - 845, 2005.
ISNAD	HİSAR, Olcay vd. "The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo". (2005), 841-845.

APA	HİSAR O, BEYDEMİR Ş, Gulcin i, ARAS H, YANIK P, KUFREVIOGLU O (2005). The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo. Turkish Journal of Veterinary and Animal Sciences, 29(3), 841 - 845.
Chicago	HİSAR Olcay,BEYDEMİR Şükrü,Gulcin ilhami,ARAS Hisar Şükriye,YANIK PROF. DR.,KUFREVIOGLU Omer Irfan The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo. Turkish Journal of Veterinary and Animal Sciences 29, no.3 (2005): 841 - 845.
MLA	HİSAR Olcay,BEYDEMİR Şükrü,Gulcin ilhami,ARAS Hisar Şükriye,YANIK PROF. DR.,KUFREVIOGLU Omer Irfan The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo. Turkish Journal of Veterinary and Animal Sciences, vol.29, no.3, 2005, ss.841 - 845.
AMA	HİSAR O,BEYDEMİR Ş,Gulcin i,ARAS H,YANIK P,KUFREVIOGLU O The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo. Turkish Journal of Veterinary and Animal Sciences. 2005; 29(3): 841 - 845.
Vancouver	HİSAR O,BEYDEMİR Ş,Gulcin i,ARAS H,YANIK P,KUFREVIOGLU O The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo. Turkish Journal of Veterinary and Animal Sciences. 2005; 29(3): 841 - 845.
IEEE	HİSAR O,BEYDEMİR Ş,Gulcin i,ARAS H,YANIK P,KUFREVIOGLU O "The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo." Turkish Journal of Veterinary and Animal Sciences, 29, ss.841 - 845, 2005.
ISNAD	HİSAR, Olcay vd. "The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo". Turkish Journal of Veterinary and Animal Sciences 29/3 (2005), 841-845.