Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions

Can, Fatma Ezgi; BAL, Salih Haldun; Göral, Güher; Budak, Ferah; ORAL, HALUK BARBAROS; HEPER, YASEMIN; Kumaş, Levent Tufan

doi:10.25002/tji.2020.1242

Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions

Salih Haldun BAL, (Bursa Uludağ Üniversitesi, Tıp Fakültesi, Dr. Raşit Durusoy Kan Merkezi, Bursa, Türkiye)

Levent Tufan KUMAŞ, (Bursa Uludağ Üniversitesi, Tıp Fakültesi, Dr. Raşit Durusoy Kan Merkezi, Bursa, Türkiye)

Yasemin HEPER, (Bursa Uludağ Üniversitesi, Tıp Fakültesi, Dr. Raşit Durusoy Kan Merkezi, Bursa, Türkiye)

Ferah BUDAK, (Bursa Uludağ Üniversitesi, Tıp Fakültesi, İmmünoloji Anabilim Dalı, Bursa, Türkiye)

Güher GÖRAL, (Bursa Uludağ Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Bursa, Türkiye)

Fatma Ezgi CAN, (Bursa Uludağ Üniversitesi, Tıp Fakültesi, Biyoistatistik Anabilim Dalı, Bursa, Türkiye)

Haluk Barbaros ORAL (Bursa Uludağ Üniversitesi, Tıp Fakültesi, İmmünoloji Anabilim Dalı, Bursa, Türkiye)

Turkish Journal of Immunology

1 0

Yıl: 2020 Cilt: 8 Sayı: 2 Sayfa Aralığı: 44 - 49 Metin Dili: İngilizce DOI: 10.25002/tji.2020.1242 İndeks Tarihi: 28-12-2021

Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions

Öz:

Introduction: Some immunologic changes in the recipient derived by allogeneic blood transfusion (ABT) are called Transfusion Related Immune Modulation (TRIM). Despite the exact mechanisms of these changes are not known, it is thought that ABT causes a decrease of CD4/CD8 ratio in the recipient. This study aimed to determine the CD4/CD8 ratio in stored erythrocyte suspensions (ES) and to obtain new information about TRIM mechanisms. Materials and Methods: Whole blood components used in our study were collected from 10 healthy volunteers. ES’ obtained from whole blood were divided into three equal aliquots. Test samples which were related to 0th, 21st and 42nd storage days were prepared from these aliquots. CD3, CD4 and CD8 surface markers in peripheral blood mononuclear cells were investigated with flow-cytometer in these test samples. Results: Our data were evaluated according to storage days. Decrease of CD3 (p=0,001), CD4 (p<0,001), CD8 (p=0,012) expressing mononuclear blood cells and helper T cells in 21st-day samples, CD4 (p=0,035) and CD8 (p=0,017) expressing cells in 42nd-day samples compared to Day 0 samples and increase of CD3 (p=0,027) expressing cells in 42nd-day samples compared to 21st-day samples were found statistically significant. Conclusion: In our study, we did not find any significant change in the ratio of CD4+ /CD8+cells in ES. Detailed studies can help us to obtain more comprehensive knowledge on this field.

Anahtar Kelime:

Eritrosit Süspansiyonlarında Depolanma Sürecinin CD4+/CD8+ T Lenfositleri Oranı Üzerine Etkisi

Öz:

Giriş: Allojeneik kan transfüzyonunun (AKT) alıcıda neden olduğu bazı immünolojik değişikliklere transfüzyonla ilişkili immunomodülasyon (TRIM) adı verilmektedir. Bu değişikliklerin mekanizması tam olarak bilinmese de, AKT’nin alıcının mononülear kan hücrelerindeki CD4/CD8 ifadesi oranında azalmaya yol açtığına inanılmaktadır. Bu çalışmada depolanan eritrosit süspansiyonlarından elde edilen mononükleer hücrelerdeki (ES) CD4/CD8 ifadesi oranını belirlemek ve TRIM mekanizmalarına yönelik yeni bilgiler edinmek amaçlanmıştır. Gereç ve Yöntemler: Çalışmamızda kullanılan tam kanlar sağlıklı 10 gönüllüden elde edilmiştir. Bu tam kanlardan elde edilen ES’ler eşit üç parçaya bölünmüş ve ES’lerin 0, 21 ve 42. raf günlerine yönelik test örnekleri bu parçalardan elde edilmiştir. Test örneklerdeki CD3, CD4 ve CD8 yüzey belirteçleri akan hücre ölçer yardımıyla incelenmiştir. Bulgular: Verilerimiz ES’lerin depolanma günlerine gore değerlendirilmiştir. Sıfırıncı gün örneklerine göre 21. gün örneklerinde CD3 (p=0,001), CD4 (p<0,001), CD8 (p=0,012) ifade eden hücrelerde ve yardımcı T hücrelerinde görülen azalma (p<0,001); 42. gün örneklerinde CD4 (p=0,035) ve CD8 (p=0,017) eksprese eden hücrelerde görülen azalma; 21. gün örneklerine göre 42. gün örneklerinde CD3 (p=0,027) ifade eden hücrelerde görülen artış istatistiksel olarak anlamlı bulunmuştur. Sonuç: Çalışmamızda ES’ler içerisinde CD4+ /CD8+hücre oranında istatistiksel olarak anlamlı bir değişiklik saptanmamıştır. Bu konuda daha kapsamlı bilgiye sahip olmak için daha detaylı çalışmalara ihtiyaç vardır.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BAL S, Kumaş L, HEPER Y, Budak F, Göral G, Can F, ORAL H (2020). Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions. , 44 - 49. 10.25002/tji.2020.1242
Chicago	BAL Salih Haldun,Kumaş Levent Tufan,HEPER YASEMIN,Budak Ferah,Göral Güher,Can Fatma Ezgi,ORAL HALUK BARBAROS Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions. (2020): 44 - 49. 10.25002/tji.2020.1242
MLA	BAL Salih Haldun,Kumaş Levent Tufan,HEPER YASEMIN,Budak Ferah,Göral Güher,Can Fatma Ezgi,ORAL HALUK BARBAROS Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions. , 2020, ss.44 - 49. 10.25002/tji.2020.1242
AMA	BAL S,Kumaş L,HEPER Y,Budak F,Göral G,Can F,ORAL H Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions. . 2020; 44 - 49. 10.25002/tji.2020.1242
Vancouver	BAL S,Kumaş L,HEPER Y,Budak F,Göral G,Can F,ORAL H Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions. . 2020; 44 - 49. 10.25002/tji.2020.1242
IEEE	BAL S,Kumaş L,HEPER Y,Budak F,Göral G,Can F,ORAL H "Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions." , ss.44 - 49, 2020. 10.25002/tji.2020.1242
ISNAD	BAL, Salih Haldun vd. "Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions". (2020), 44-49. https://doi.org/10.25002/tji.2020.1242

APA	BAL S, Kumaş L, HEPER Y, Budak F, Göral G, Can F, ORAL H (2020). Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions. Turkish Journal of Immunology, 8(2), 44 - 49. 10.25002/tji.2020.1242
Chicago	BAL Salih Haldun,Kumaş Levent Tufan,HEPER YASEMIN,Budak Ferah,Göral Güher,Can Fatma Ezgi,ORAL HALUK BARBAROS Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions. Turkish Journal of Immunology 8, no.2 (2020): 44 - 49. 10.25002/tji.2020.1242
MLA	BAL Salih Haldun,Kumaş Levent Tufan,HEPER YASEMIN,Budak Ferah,Göral Güher,Can Fatma Ezgi,ORAL HALUK BARBAROS Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions. Turkish Journal of Immunology, vol.8, no.2, 2020, ss.44 - 49. 10.25002/tji.2020.1242
AMA	BAL S,Kumaş L,HEPER Y,Budak F,Göral G,Can F,ORAL H Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions. Turkish Journal of Immunology. 2020; 8(2): 44 - 49. 10.25002/tji.2020.1242
Vancouver	BAL S,Kumaş L,HEPER Y,Budak F,Göral G,Can F,ORAL H Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions. Turkish Journal of Immunology. 2020; 8(2): 44 - 49. 10.25002/tji.2020.1242
IEEE	BAL S,Kumaş L,HEPER Y,Budak F,Göral G,Can F,ORAL H "Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions." Turkish Journal of Immunology, 8, ss.44 - 49, 2020. 10.25002/tji.2020.1242
ISNAD	BAL, Salih Haldun vd. "Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions". Turkish Journal of Immunology 8/2 (2020), 44-49. https://doi.org/10.25002/tji.2020.1242