Objectives: In this study, we aimed to investigate the effect oftranexamic acid (TXA) on osteotendinous junction healing in arat model, both biomechanically and histologically. Materials and methods: Sixty-four male Wistar-Albino ratsweighing 450 to 600 g were used in this study. The ratswere divided into two groups as the experimental (n=16) andcontrol (n=16) groups. Achillotomy and subsequent repairsite was exposed to 1 mL of TXA in the experimental group,while 1 mL of saline was given to the control group. Forbiomechanical and histopathological investigation, each groupwas further divided into two subgroups. At the end of fourweeks, all rats were sacrificed. Biomechanical tests wereperformed using the M500-50CT device. The Bonar, Movin,and Nourissat bone-tendon junction scoring systems were usedfor histopathological evaluation. Results: There was no statistically significant differencein the elongation at a maximum point, maximum loading,and maximum stress variables in the biomechanical study(p=0.558 p=0.775, and p=0.558, respectively). In thehistopathological evaluation, the collagen content and layoutwere close to the native tissue in the experimental group(p=0.047 and p=0.008, respectively). Vascularity, hyalinization,and glycosaminoglycan content were significantly lower inthe experimental group (p=0.004, p=0.014, and p=0.026,respectively). The total Bonar and Movin scores were morefavorable in the experimental group (p<0.001). Conclusion: This experimental study showed that localadministration of TXA accelerated bone-tendon junction healingin rats.