Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality
Yıl: 2020 Cilt: 13 Sayı: 4 Sayfa Aralığı: 383 - 387 Metin Dili: İngilizce DOI: 10.30607/kvj.750279 İndeks Tarihi: 03-01-2022
Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality
Öz: In the present study we investigate the effects of parthenogenetic activation on in vitro embryo development and quality in different activation periods. oocytes were obtained 14 hours after human chorionic gonadotropin (hCG) injection from superovulated B6D2F1 female mice then parthenogenetic activation started 18 hours after hCG injection. The oocytes were activated at different activation periods for 3, 4, 5 or 6 hours in 10 mM strontium chloride $(SrCl_2)$ + 5 $μg/mL^{-1}$ Cytohalasine B (CB)+5 nM Trichostatin A (TSA) containing a $Ca ^{2+}$ free Chatot Ziomek Brinster (CZB) activation medium, followed by further incubation for two hours at 37°C and 5% $CO_2$ in embryo culturing medium + TSA. The results in the present study suggested that the parthenogenetic activation of the 6 hour activation period was found to be higher than at 3, 4 and 5 hours.
Anahtar Kelime: Farklı Partenogenetik Aktivasyon Sürelerinin Fare Embriyo Gelişimi ve Kalitesi Üzerine Etkileri
Öz: Çalışmamızın amacı, partenogenetik aktivasyonda farklı aktivasyon sürelerinin in vitro embriyo gelişimi ve kalitesi üzerindeki etkilerinin araştırılmasıdır. Superovule B6D2F1 ırkı dişi farelere uygulanan insan koryonik gonadotropin (hCG) enjeksiyonundan 14 saat sonra oositler elde edildi ve 18 saat sonra partenogenetik aktivasyona başlandı. Oositler, 10 mM stronsiyum klorür $(SrCl_2)$ + 5 $μg/mL^{-1}$ sitokalazin B (CB) + 5 nM trikostatin A (TSA) $Ca ^{2+}$ içermeyen Chatot Ziomek Brinster (CZB) medyumu içerisinde 3, 4, 5 ve 6 saat bekletildi. Aktivasyon sonrası, embriyo kültür medyumu + TSA’da inkübatörde 37°C ve %5 $CO_2$ ortamında 2 saat bekletildi. Son olarak, tüm embriyolar 120 saat süre ile kültüre edildi. Bu çalışmadan elde edilen sonuçlar göre, 6 saatlik partenogenetik aktivasyon başarısının, 3, 4 ve 5 saatlik sürelere göre daha yüksek olduğu saptandı.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Taskin A, Coskun N, Kocabay A (2020). Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality. , 383 - 387. 10.30607/kvj.750279 |
| Chicago | Taskin Ali Cihan,Coskun Nilhan,Kocabay Ahmet Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality. (2020): 383 - 387. 10.30607/kvj.750279 |
| MLA | Taskin Ali Cihan,Coskun Nilhan,Kocabay Ahmet Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality. , 2020, ss.383 - 387. 10.30607/kvj.750279 |
| AMA | Taskin A,Coskun N,Kocabay A Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality. . 2020; 383 - 387. 10.30607/kvj.750279 |
| Vancouver | Taskin A,Coskun N,Kocabay A Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality. . 2020; 383 - 387. 10.30607/kvj.750279 |
| IEEE | Taskin A,Coskun N,Kocabay A "Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality." , ss.383 - 387, 2020. 10.30607/kvj.750279 |
| ISNAD | Taskin, Ali Cihan vd. "Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality". (2020), 383-387. https://doi.org/10.30607/kvj.750279 |
| APA | Taskin A, Coskun N, Kocabay A (2020). Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality. Kocatepe Veterinary Journal, 13(4), 383 - 387. 10.30607/kvj.750279 |
| Chicago | Taskin Ali Cihan,Coskun Nilhan,Kocabay Ahmet Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality. Kocatepe Veterinary Journal 13, no.4 (2020): 383 - 387. 10.30607/kvj.750279 |
| MLA | Taskin Ali Cihan,Coskun Nilhan,Kocabay Ahmet Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality. Kocatepe Veterinary Journal, vol.13, no.4, 2020, ss.383 - 387. 10.30607/kvj.750279 |
| AMA | Taskin A,Coskun N,Kocabay A Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality. Kocatepe Veterinary Journal. 2020; 13(4): 383 - 387. 10.30607/kvj.750279 |
| Vancouver | Taskin A,Coskun N,Kocabay A Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality. Kocatepe Veterinary Journal. 2020; 13(4): 383 - 387. 10.30607/kvj.750279 |
| IEEE | Taskin A,Coskun N,Kocabay A "Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality." Kocatepe Veterinary Journal, 13, ss.383 - 387, 2020. 10.30607/kvj.750279 |
| ISNAD | Taskin, Ali Cihan vd. "Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality". Kocatepe Veterinary Journal 13/4 (2020), 383-387. https://doi.org/10.30607/kvj.750279 |
