Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis

Öztan, Meltem Dartar; Kiyan, Mehmet; Basmaci, Fatma

doi:10.5152/cjms.2021.896

Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis

Fatma KERMEOĞLU, (Yakın Doğu Üniversitesi, Diş Hekimliği Fakültesi, Endodonti Anabilim Dalı, Lefkoşa, Kıbrıs)

Meltem DARTAR ÖZTAN, (Ankara Üniversitesi, Diş Hekimliği Fakültesi, Endodonti Anabilim Dalı, Ankara, Türkiye)

Mehmet KIYAN (Ankara Üniversitesi, Tıp Fakültesi, Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye)

Cyprus Journal of Medical Sciences

3 0

Yıl: 2021 Cilt: 6 Sayı: 2 Sayfa Aralığı: 151 - 156 Metin Dili: İngilizce DOI: 10.5152/cjms.2021.896 İndeks Tarihi: 05-01-2022

Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis

Öz:

BACKGROUND/AIMS The purpose of this study was to examine the ability of gutta-percha points to reduce bacterial counts. MATERIAL and METHODS ISO #40-sized Calcium Hydroxide Plus Points or Activ Points were used for all experiments. Three pieces of each type of gutta-percha points were placed in sterile 0.5-mL eppendorf tubes. After incubating the plates in an anaerobic chamber at 37 C for 2 days, colony forming units’ (CFUs) numbers per milliliter of suspension were calculated, and CFU numbers relating to each time interval were statistically analyzed by using Kruskal–Wallis 1-way analysis of variance and multiple comparison tests. RESULTS The results showed that Calcium Hydroxide Plus Points or Activ Points have similar effects to kill the tested microorganisms except Enterococcus faecalis and Peptostreptococcus micros. CONCLUSION Calcium Hydroxide Plus Points or Activ Points have similar effects to kill the tested microorganisms and did not kill 100% of E. faecalis and P. micros.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Souse ELR, Ferraz CCR, Gomes BPFA, Pinheiro ET, Teixeira FB, Souza-Filho FJ. Bacteriological study of root canals associated with periapical abscesses. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;96(3):332-339. [CrossRef]
2. Siqueira JF, Roc¸as IN, Soutu R, Uzeda M, Colombo AP. Microbiological evaluation of acute periradicular abscesses by DNA-DNA hybridization. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001;92(4):451-457. [CrossRef]
3. Sundqvist G, Johansson E, Sjo¨gren U. Prevalence of blackpigmented bacteroides species in root canal infections. J Endod. 1989;15(1):13-19. [CrossRef]
4. Murray CA, Saunders WP. Root canal treatment and general health: A reviewof the literature. Int Endod J. 2000;33(1):1-18. [CrossRef]
5. Sjo¨gren U, Figdor D, Spangberg L, Sundqvist G. The antimicrobial effect of calcium hydroxide as a short-term intracanal dressing. Int Endod J. 1991;24(3):119-125. [CrossRef]
6. Bystro¨m A, Claesson R, Sundqvist G. The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canals. Endod Dent Traumatol. 1985;1(5):170-175. [CrossRef]
7. Barthel C, Levin L, Reisner H, Trope M. TNF-alpha release in monocytes after exposure to calcium hydroxide treated Escherichia coli LPS. Int Endod J. 1997;30:155-159.
8. Aibel K, Stevens R. Effect of chlorhexidine on IL-6 induction by LPS. J Endod. 1999;25(4):282. [CrossRef]
9. Hennessy T. Some antibacterial properties of chlorhexidine. J Periodontol Res. 1973;8(s12):61-67. [CrossRef]
10. Emilson C. Susceptibility of various microorganisms to chlorhexidine. Scand J Dent Res. 1977;85(4):255-265. [CrossRef]
11. Podbielski A, Boeckh C, Haller B. Growth inhibitory activity of gutta-percha points containing root canal medications on common endodontic bacterial pathogens as determined by an optimized quantitative in vitro assay. J Endod. 2000;26(7):398-403. [CrossRef]
12. Oztan MD, Kiyan M, Gerceker D. Antimicrobial effect, in vitro, of gutta-percha points containing root canal medications against yeasts and Enterococcus faecalis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;102(3):410-416. [CrossRef]
13. Sato T, Yamaki K, Ishida N, et al. Cultivable anaerobic microbiota of infected root canals. Int J Dent. 2012;2012:1-5. [Cross- Ref]
14. Melker KB, Vertucci FJ, Rojas MF, Progulske-Fox A, Be´langer M. Antimicrobial efficacy of medicated root canal filling materials. J Endod. 2006;32(2):148-151. [CrossRef]
15. Vrcek D, Prpic-Mehicic G, Tambic-Andrasevic A, Poljak-Guberina R, Galic N, Katunaric M. The antimicrobial effect of Calasept, Superlux calcium hydroxide liner and gutta-percha with calcium hydroxide. Acta Stomat Croat. 2002;36:209-212.
16. Tandan M, Hegde MN, Hegde P. Effect of four different intracanal medicaments on the apical seal of the root canal system: A dye extraction study. Indian J Dent Res. 2014;25(5):607- 612. [CrossRef]
17. Tanomaru JMG, Pappen FG, Filho MT, Spolidorio DMP, Ito IY. In vitro antimicrobial activity of different gutta-percha points and calcium hydroxide pastes. Braz Oral Res. 2007;21:35-39. [CrossRef]
18. Ebert J, Roggendorf MJ, Frank K, Petschelt A. Antimicrobial activity of various ‘active’ gutta-percha points against Enterococcus faecalis in simulated root canals. Int Endod J. 2008;41(3):249-257. [CrossRef]
19. Podbielski A, Spahr A, Haller B. Additive antimicrobial activity of calcium hydroxide and chlorhexidine on common endodontic bacterial pathogens. J Endod. 2003;29(5):340-345. [CrossRef]
20. Stojanovic N, Krunic J, Popovic B, Stojicic S, Zivkovic S. Prevalence of Enterococcus faecalis and Porphyromonas gingivalis in infected root canals and their susceptibility to endodontic treatment procedures: A molecular study. Srp Arh Celok Lek. 2014;142(9-10):535-541. [CrossRef]
21. Jhamb A, Chaurasia VR, Masamatti VK, Agarwal JH, Tiwari S, Nair D. In vitro evaluation of antimicrobial activity of different gutta-percha points and calcium hydroxide pastes. J Int Soc Prev Community Dent. 2014;4(2):92-95. [CrossRef]
22. Barthel CR, Zimmer S, Zilliges S, Schiller R, Gobel UB, Roulet JF. In situ antimicrobial effectiveness of chlorhexidine and calcium hydroxide: Gel and paste versus gutta-percha points. J Endod. 2002;28(6):427-430. [CrossRef]
23. Lenet B, Komorowski R, Wu X, et al. Antimicrobial substantivity of bovine root dentin exposed to different chlorhexidine delivery vehicles. J Endod. 2000;26(11):652-655. [CrossRef]
24. Evans M, Davies JK, Sundqvist G, Figdor D. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J. 2002;35(3):221-228. [CrossRef]
25. Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: Its role in root canal treatment failure and current concepts in retreatment. J Endod. 2006;32(2):93-98. [CrossRef]
26. Al-Nazhan S. Antimicrobial activity of extracts of calcium hydroxide points. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002;93(5):593-595. [CrossRef]
27. Lui JN, Sae-Lim V, Song KP, Chen NN. In vitro antibacterial effect of chlorhexidine impregnated gutta-percha points on Enterococcus faecalis. Int Endod J. 2004;37(2):105-113. [Cross- Ref]
28. Rathke A, Meisohle D, Bokelmann J, Haller B. Antibacterial activity of calcium hydroxide and chlorhexidine containing points against Fusobacterium nucleatum and Parvimonas micra. Eur J Dent. 2012;6(4):434-439. [CrossRef]
29. Bozza FL, Molgatini SL, Pe´rez SB, et al. Antimicrobial effect in vitro of chlorhexidine and calcium hydroxide impregnated gutta-percha points. Acta Odontol Latinoam. 2005;18:51-56.
30. Schafer E, Al Behaissi A. pH changes in root dentin after root canal dressing with gutta-percha points containing calcium hydroxide. J Endod. 2000;26(11):665-667. [CrossRef]

APA	Basmaci F, Öztan M, Kiyan M (2021). Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis. , 151 - 156. 10.5152/cjms.2021.896
Chicago	Basmaci Fatma,Öztan Meltem Dartar,Kiyan Mehmet Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis. (2021): 151 - 156. 10.5152/cjms.2021.896
MLA	Basmaci Fatma,Öztan Meltem Dartar,Kiyan Mehmet Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis. , 2021, ss.151 - 156. 10.5152/cjms.2021.896
AMA	Basmaci F,Öztan M,Kiyan M Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis. . 2021; 151 - 156. 10.5152/cjms.2021.896
Vancouver	Basmaci F,Öztan M,Kiyan M Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis. . 2021; 151 - 156. 10.5152/cjms.2021.896
IEEE	Basmaci F,Öztan M,Kiyan M "Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis." , ss.151 - 156, 2021. 10.5152/cjms.2021.896
ISNAD	Basmaci, Fatma vd. "Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis". (2021), 151-156. https://doi.org/10.5152/cjms.2021.896

APA	Basmaci F, Öztan M, Kiyan M (2021). Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis. Cyprus Journal of Medical Sciences, 6(2), 151 - 156. 10.5152/cjms.2021.896
Chicago	Basmaci Fatma,Öztan Meltem Dartar,Kiyan Mehmet Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis. Cyprus Journal of Medical Sciences 6, no.2 (2021): 151 - 156. 10.5152/cjms.2021.896
MLA	Basmaci Fatma,Öztan Meltem Dartar,Kiyan Mehmet Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis. Cyprus Journal of Medical Sciences, vol.6, no.2, 2021, ss.151 - 156. 10.5152/cjms.2021.896
AMA	Basmaci F,Öztan M,Kiyan M Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis. Cyprus Journal of Medical Sciences. 2021; 6(2): 151 - 156. 10.5152/cjms.2021.896
Vancouver	Basmaci F,Öztan M,Kiyan M Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis. Cyprus Journal of Medical Sciences. 2021; 6(2): 151 - 156. 10.5152/cjms.2021.896
IEEE	Basmaci F,Öztan M,Kiyan M "Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis." Cyprus Journal of Medical Sciences, 6, ss.151 - 156, 2021. 10.5152/cjms.2021.896
ISNAD	Basmaci, Fatma vd. "Antimicrobial Effects of Gutta-Percha Points Containing Root Canal Medications against Some Anaerobic Bacterial Species and Enterococcus faecalis". Cyprus Journal of Medical Sciences 6/2 (2021), 151-156. https://doi.org/10.5152/cjms.2021.896