Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder

kurhan, faruk; alp, hamit hakan

doi:10.5455/apd.10091

Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder

Faruk KURUHAN, (Yüzüncü Yıl Üniversitesi Tıp Fakültesi, Ruh Sağlığı ve Hastalıkları Anabilim Dalı, Van, Türkiye)

Hamit Hakan ALP (Yüzüncü Yıl Üniversitesi Tıp Fakültesi, Biyokimya Bölümü, Van, Türkiye)

Alpha psychiatry (Online)

6 1

Yıl: 2021 Cilt: 22 Sayı: 3 Sayfa Aralığı: 123 - 129 Metin Dili: İngilizce DOI: 10.5455/apd.10091 İndeks Tarihi: 07-01-2022

Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder

Öz:

Objective: There are few studies on oxidative stress in patients with post-traumatic stress disorder(PTSD). The thiol/disulfide homeostasis is a new marker of oxidative stress. This study aimed to examinethe oxidative DNA damage and thiol/disulfide homeostasis after 6 months in patients who developedPTSD after an avalanche disaster and to compare them with healthy controls. Methods: A total of 31 patients who developed PTSD after 2 consecutive avalanche disasters that oc curred in Van on February 4 and 5, 2020, resulting in 42 deaths, and 33 healthy volunteers were includ ed in the study. The patients were followed up by a psychiatrist within the framework of psychosocialintervention during their admission to Yüzüncü Yil University Medical Faculty Emergency Service. Thepatients monitored for a long time were diagnosed according to DSM-5 diagnostic criteria. The clinicalfollow-up was evaluated with the post-traumatic stress disorder self-assessment (PTSD-KD) and the im pact of events scale. To determine oxidative DNA damage, 8-hydroxy-2-deoxyguanosine (8-OHdG) anddeoxyguanosine (dG) levels were determined by isolating leukocyte DNA. Oxidative DNA damage wasgiven as a ratio of 8-OHdG/106dG. Total thiol/native thiol levels were also determined. Disulfide levelswere calculated by subtracting native thiol results from the total thiol results and dividing them by 2. Results: It was determined that total thiol and native thiol levels in patients with PTSD were statisticallysignificantly lower than in the healthy control group (P = .001), and the disulfide levels were higher inthe PTSD group compared with that in the healthy control group (P = .001). In addition, 8-OHdG, anindicator of DNA damage, was found to be significantly lower in the control group than in the patientgroup (P = .001). Conclusion: In our study, thiol/disulfide homeostasis was observed to shift toward disulfide in patientswith PTSD when compared with healthy controls. The level of 8-OHdG, the indicator of DNA damage,was observed to increase in patients with PTSD. This result indicates that thiol/disulfide homeostasis canbe significant in the pathophysiology of oxidative stress in these patients.

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1. Guha-Sapir D, Hoyois P. Estimating populations affected by disasters: a review of methodological issues and research gaps. Brussels: Centre for Research on the Epidemiology of Disasters (CRED). Institute of Health and Society (IRSS). University Catholique de Louvain; 2015.
2. Goldmann E, Galea S. Mental health consequences of disasters. Annu Rev Public Health. 2014;35:169-183. [Crossref]
3. McFarlane AC, Norris FH. Definitions and concepts in disaster research. Methods for Disaster Mental Health Research. 2006;2006:3-19.
4. Dattilo NC. Posttraumatic stress disorder. In: Kellerman RD, Bope ED, eds. Conn’s Current Therapy. Philadelphia, PA: Elsevier; 2018:784-787.
5. Miller GE, Chen E, Parker KJ. Psychological stress in childhood and susceptibility to the chronic diseases of aging: moving toward a model of behavioral and biological mechanisms. Psychol Bull. 2011;137(6):959. [Crossref]
6. Aschbacher K, O’Donovan A, Wolkowitz OM, Dhabhar FS, Su Y, Epel E. Good stress, bad stress and oxidative stress: insights from anticipatory cortisol reactivity. Psychoneuroendocrinology. 2013;38(9):1698-1708. [Crossref]
7. Wimalawansa SJ. Mechanisms of developing post-traumatic stress disorder: new targets for drug development and other potential interventions. CNS Neurol Disord Drug Targets. 2014;13(5):807-816. [Crossref]
8. Su YA, Wu J, Zhang L, et al. Dysregulated mitochondrial genes and networks with drug targets in postmortem brain of patients with posttraumatic stress disorder (PTSD) revealed by human mitochondria-focused cdna microarrays. Int J Biol Sci. 2008;4(4):223. [Crossref]
9. Şimşek Ş, Yüksel T, Kaplan İ, Uysal C, Aktaş H. The levels of cortisol and oxidative stress and DNA damage in child and adolescent victims of sexual abuse with or without post-traumatic stress disorder. Psychiatry Investig. 2016;13(6):616. [Crossref]
10. Erzin G, Kotan VO, Topçuoğlu C, et al. Thiol/disulphide homeostasis in bipolar disorder. Psychiatry Res. 2018;261:237-242. [Crossref]
11. Attari A, Asgari S, Naderi GA, Rezayat A. Lipid peroxidation and antioxidant capacity in posttraumatic stress disorder. J Res Med Sci. 2003;8(1).
12. Selek S, Herken H, Bulut M, et al. Oxidative imbalance in obsessive compulsive disorder patients: a total evaluation of oxidant-antioxidant status. Prog Neuropsychopharmacol Biol Psychiatry. 2008;32(2):487-491. [Crossref]
13. Altan N, Dinçel AS, Koca C. Diyabetes mellitus and oxidative stress. Turk J Biochem. 2006;31(2):51-56.
14. Berg D, Youdim MB, Riederer P. Redox imbalance. Cell Tissue Res. 2004;318(1):201-213. [Crossref]
15. Maes M, Kubera M, Leunis JC, Berk M, Geffard M, Bosmans E. In depression, bacterial translocation may drive inflammatory responses, oxidative and nitrosative stress (O&NS), and auto-immune responses directed against O&NS‐damaged neoepitopes. Acta Psychiatr Scand. 2013;127(5):344-354. [Crossref]
16. Ciobica A, Padurariu M, Dobrin I, Stefanescu C, Dobrin R. Oxidative stress in schizophrenia-focusing on the main markers. Psychiatria Danubina. 2011;23(3):237-245.
17. Pavlović D, Tamburić V, Stojanović I, Kocić G, Jevtović T, Đorđević V. Oxidative stress as marker of positive symptoms in schizophrenia. Facta Univ. 2002;9:157-161.
18. Kayalı R, Çakatay U. Basic mechanisms of protein oxidation. Cerrahpaşa J Med. 2004;35(2):83-89.
19. Biswas S, Chida AS, Rahman I. Redox modifications of protein-thiols: emerging roles in cell signaling. Biochem Pharmacol. 2006;71(5):551-564. [Crossref]
20. Circu ML, Aw TY. Reactive oxygen species, cellular redox systems, and apoptosis. Free Radic Biol Med. 2010;48(6):749-762. [Crossref]
21. Milenkovic D, Jude B, Morand C. Mirna as molecular target of polyphenols underlying their biological effects. Free Radic Biol Med. 2013;64:40- 51. [Crossref]
22. Topcuoglu C, Bakirhan A, Yilmaz FM, et al. Thiol/disulfide homeostasis in untreated schizophrenia patients. Psychiatry Res. 2017;251:212-216. [Crossref]
23. Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem. 2014;47(18):326-332. [Crossref]
24. Vural G, Gumusyayla S, Bektas H, Deniz O, Alisik M, Erel O. Impairment of dynamic thiol-disulphide homeostasis in patients with idiopathic Parkinson’s disease and its relationship with clinical stage of disease. Clin Neurol Neurosurg. 2017;153:50-55. [Crossref]
25. Valko M, Rhodes C, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact. 2006;160(1):1-40. [Crossref]
26. Long JD, Matson WR, Juhl AR, et al. 8ohdg as a marker for huntington disease progression. Neurobiol Dis. 2012;46(3):625-634. [Crossref]
27. Yokuş B, Çakir DÜ. Biomarker of invivo oxidative DNA damage 8 hydroxy 2 deoxyguanosine. Turkiye Klinikleri J Med Sci. 2002;22(5):535-543.
28. Foa EB, Riggs DS, Dancu CV, Rothbaum BO. Reliability and validity of a brief instrument for assessing post‐traumatic stress disorder. J Trauma Stress. 1993;6(4):459-473. [Crossref]
29. Aydin A, Barut Y, Kalafat T, Boysan M, Besiroglu L. Psychometric properties of the Turkish version of the PTSD Symptom Scale-Self-Report (PSSSR). Anadolu Psikiyatri Derg. 2012;13(2):125-130.
30. Çorapçıoğlu A, Yargıç İ, Geyran P, Kocabaşoğlu N. Olayların Etkisi Ölçeği, IES-R) Türkçe versiyonunun geçerlilik ve güvenilirliği [Validity and reliability of Turkish version of “Impact of Event Scale-Revised” (IES-R)]. New Symposium Journal. 2006;44(1);14-22.
31. Kaur H, Halliwell B. Measurement of oxidized and methylated DNA bases by HPLC with electro-chemical detection. Biochem J. 1996;318(1):21-23. [Crossref]
32. Shigenaga MK, Aboujaoude EN, Chen Q, Ames BN. [2] assays of oxidative DNA damage bio-markers 8-oxo-2’-deoxyguanosine and 8-oxo-guanine in nuclear DNA and biological fluids by high-performance liquid chromatography with electrochemical detection. Methods Enzymol.1994;234:16-33. [Crossref]
33. Ates I, Kaplan M, Yuksel M, et al. Determination of thiol/disulphide homeo-stasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine. 2016;51(1):47-51. [Crossref]
34. Altıparmak IH, Erkuş ME, Sezen H, et al. The relation of serum thiol levels and thiol/disulphide homeostasis with the severity of coronary artery disease. Kardiol Pol. 2016;74(11):1346-1353. [Crossref]
35. Epel ES, Blackburn EH, Lin J, et al. Accelerated telomere shortening in response to life stress. Proc Natl Acad Sci USA. 2004;101(49):17312-17315. [Crossref]
36. Nakhaee A, Shahabizadeh F, Erfani M. Protein and lipid oxidative damage in healthy students during and after exam stress. Physiol Behav. 2013;118:118-121. [Crossref]
37. Peña-Bautista C, Tirle T, López-Nogueroles M, Vento M, Baquero M, Cháfer-Pericás C. Oxidative damage of DNA as early marker of alzheimer’s disease. Int J Mol Sci. 2019;20(24):6136. [Crossref]
38. Hovatta I, Tennant RS, Helton R, et al. Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice. Nature. 2005; 438(7068):662-666. [Crossref]
39. Haddad JJ, Saadé NE, Safieh-Garabedian B. Cytokines and neuro-immune-endocrine interactions: a role for the hypothalamic-pituitary-adrenal revolving axis. J Neuroimmunol. 2002;133(1-2):1-19. [Crossref]
40. Terawaki H, Terada T, Ogura M, Era S, Hosoya T. The elevation of oxidative stress after the great east japan earthquake. Clin Exp Nephrol. 2012;16(5):816-817. [Crossref]
41. Maes M, Kubera M, Obuchowiczwa E, Goehler L, Brzeszcz J. Depression’s multiple comorbidities explained by (neuro) inflammatory and oxidative & nitrosative stress pathways. Neuroendocrinol Lett. 2011;32(1):7-24.
42. Andreazza AC, Kauer-Sant’Anna M, Frey BN, et al. Oxidative stress markers in bipolar disorder: a meta-analysis. J Affect Disord. 2008;111(2-3):135- 144. [Crossref]
43. Kösem A, Yücel Ç, Titiz AP, et al. Evaluation of serum thiol-disulphide homeostasis parameters as oxidative stress markers in epilepsy patients. Acta Neurol Belg. Published online Jun 14, 2020. doi: 10.1007/s13760- 020-01410-6. [Crossref]
44. Alagoz AN, Tasdemir SS, Aras YG, Can NU. Thiol disulfide homeostasis as oxidative stress marker in migraine patients. J Exp Clin Med. 2019;36(1):1- 8.
45. Asoğlu M, Kilicaslan F, Beginoğlu Ö, et al. Thiol/disulphide homeostasis as a new oxidative stress marker in untreated patients with generalized anxiety disorder. Anadolu Psikiyatri Derg. 2018;19(2):143-149. [Crossref]
46. Nishioka N, Arnold SE. Evidence for oxidative DNA damage in the hippocampus of elderly patients with chronic schizophrenia. Am J Geriatr Psychiatry. 2004;12(2):167-175. [Crossref]
47. Forlenza MJ, Miller GE. Increased serum levels of 8-hydroxy-2’-deoxyguanosine in clinical depression. Psychosom Med. 2006;68(1):1-7. [Crossref]
48. Čeprnja M, Đerek L, Unić A, et al. Oxidative stress markers in patients with post-traumatic stress disorder. Coll Antropol. 2011;35(4):1155-1160.
49. Şimşek Ş, Gençoğlan S, Yüksel T, Kaplan İ, Alaca R, Aktaş H. Oxidative stress and DNA damage in untreated first-episode psychosis in adolescents. Neuropsychobiology. 2016;73(2):92-97. [Crossref]
50. Ahmadimanesh M, Abbaszadegan MR, Morshedi Rad D, et al. Effects of selective serotonin reuptake inhibitors on DNA damage in patients with depression. J Psychopharmacol. 2019;33(11):1364-1376. [Crossref].

APA	kurhan f, alp h (2021). Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder. , 123 - 129. 10.5455/apd.10091
Chicago	kurhan faruk,alp hamit hakan Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder. (2021): 123 - 129. 10.5455/apd.10091
MLA	kurhan faruk,alp hamit hakan Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder. , 2021, ss.123 - 129. 10.5455/apd.10091
AMA	kurhan f,alp h Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder. . 2021; 123 - 129. 10.5455/apd.10091
Vancouver	kurhan f,alp h Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder. . 2021; 123 - 129. 10.5455/apd.10091
IEEE	kurhan f,alp h "Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder." , ss.123 - 129, 2021. 10.5455/apd.10091
ISNAD	kurhan, faruk - alp, hamit hakan. "Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder". (2021), 123-129. https://doi.org/10.5455/apd.10091

APA	kurhan f, alp h (2021). Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder. Alpha psychiatry (Online), 22(3), 123 - 129. 10.5455/apd.10091
Chicago	kurhan faruk,alp hamit hakan Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder. Alpha psychiatry (Online) 22, no.3 (2021): 123 - 129. 10.5455/apd.10091
MLA	kurhan faruk,alp hamit hakan Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder. Alpha psychiatry (Online), vol.22, no.3, 2021, ss.123 - 129. 10.5455/apd.10091
AMA	kurhan f,alp h Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder. Alpha psychiatry (Online). 2021; 22(3): 123 - 129. 10.5455/apd.10091
Vancouver	kurhan f,alp h Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder. Alpha psychiatry (Online). 2021; 22(3): 123 - 129. 10.5455/apd.10091
IEEE	kurhan f,alp h "Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder." Alpha psychiatry (Online), 22, ss.123 - 129, 2021. 10.5455/apd.10091
ISNAD	kurhan, faruk - alp, hamit hakan. "Investigation of Thiol/Disulfide Balance and OxidativeDNA Damage in Patients Experiencing AvalancheDisaster and with a Diagnosis of Post-TraumaticStress Disorder". Alpha psychiatry (Online) 22/3 (2021), 123-129. https://doi.org/10.5455/apd.10091