Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines

Seda Mesci, (Hitit Üniversitesi, Bilimsel Teknik Uygulama ve Araştırma Merkezi, Çorum, Türkiye)
MELEK GÜL, (Amasya Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü, Amasya, Türkiye)
TUBA YILDIRIM (Amasya Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Amasya, Türkiye)
Clinical and Experimental Health Sciences
0 0
Yıl: 2021 Cilt: 11 Sayı: 1 Sayfa Aralığı: 81 - 90 Metin Dili: İngilizce DOI: 10.33808/clinexphealthsci.787954 İndeks Tarihi: 12-01-2022

Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines

Öz:
Objective: To investigate the antioxidant and cytotoxic activities of sulfur-containing glycine imine derivatives MCF-7 (human breast adenocarcinoma) and DLD-1 (colorectal adenocarcinoma) cell lines. Methods: This study examined the antioxidant activities (25-200 µM) of sulfur-containing glycine imine derivatives via the DPPH, metal chelating and reduction methods. Furthermore the cytotoxic activity of MCF-7, MCF-12A (normal breast epithelial) and DLD-1, CCD-18CO (normal colon fibroblast) were examined with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) and RTCA (Real-time Cell Analysis) assays. Results: The antioxidant assay of the metal chelating activity showed significant results (71, 77 and 40% respectively) as compared to knowing synthetic antioxidant (trolox; 95.45, EDTA; 97.06 %). Reducing activity was found to be very low compared to the standard compounds. Compounds were shown to be moderated by DPPH (2,2-Diphenyl-1-picrylhydrazyl) activity, and the IC50 value ranged from 91 to 150. The IC50 values (100 µM) of the MTT and RTCA analyses were similar. Conclusion: The study showed that the compounds had selective and significant antioxidant activities, and we also found that they had cytotoxic effects on MCF-7 and DLD-1 cells.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • [1] Jemal A, Siegel R, Ward E, Hao Y. Cancer Statistics, CA. Cancer J Clin. 2008;58(2):71-96.
  • [2] Siegel RL, Miller KD, Jemal A. Cancer Statistics, CA Cancer J Clin. 2017;67(1):7-30.
  • [3] World Health Organization. WHO. What is cancer?. https:// www.who.int/cancer/en/ (2018).
  • [4] Saip P, Keskin S, Özkan M, Kaplan MA, Aydoğan F, Demirağ GG, et al. The Access Rate to Dıagnosıs and Treatment Modalıtıes in Breast Cancer Patıents in Turkey; Multıcenter Observatıonal Study. J Breast Health 2011;7:109-117.
  • [5] Jemal A, Siegel R, Ward E, Hao Y, Xu J, Thu MJ. Cancer statistics, CA. Cancer J Clin 2009; 59:225-49.
  • [6] Vaya J, Aviram M. Nutritional Antioxidants: Mechanisms of Action, Analyses of Activities and Medical Applications Curr. Med. Chem. – Imm., Endoc. & Metab. Agents, 2001;1:99-117.
  • [7] Valadez-Vega C, Delgado-Olivares L, Morales González JA, Alanís García E, Villagomez Ibarra JR, Ramírez Moreno, E., et al. The role of natural antioxidants in cancer disease. En MoralesGonzález JA.(Edi.), Oxidative Stress and Chronic Degenerative Diseases – a Role for Antioxidants. 2013;391-418.
  • [8] Ganapathy V. Protein digestion and absorption. In Physiology of the Gastrointestinal Tract (Fifth Edition), Chapter 59 – Protein Digestion and Absorption, 2012;1595-1623.
  • [9] Cheng SB, Liu HT, Chen SY, Lin PT, Lai CY.Huang, YC. Changes of oxidative stress, glutathione, and its dependent antioxidant enzyme activities in patients with hepatocellular carcinoma before and after tumor resection. PloS one, 2017;12(1):e0170016.
  • [10] Deters M, Siegers CP, Strubelt O. Influence of glycine on the damage induced in isolated perfused rat liver by five hepatotoxic agents. Toxicology, 1998;128:63–72.
  • [11] Vaillancourt VA, Larsen SD, Tanis SP, Burr JE, Connell MA, Cudahy, MM. et al. Synthesis and biological activity of aminoguanidine and diaminoguanidine analogues of the antidiabetic/antiobesity agent 3-guanidinopropionic acid. J Med Chem 2001;44(8):1231-1248.
  • [12] Bruns H, Kazanavicius D, Schultze D, Saeedi MA. Yamanaka K, Strupas K, et al. Glycine inhibits angiogenesis in colorectal cancer: Role of endothelial cells. Amino Acids, 2016;48: 2549- 2558.
  • [13] Mukwevho E, Ferreira Z, Ayeleso A. Potential Role of Sulfur-Containing Antioxidant Systems in Highly Oxidative Environments. Molecules, 2014;19:19376-19389.
  • [14] Santhilkumar R, Sengottuvelan M, Nalini N. Protective effect of glycine supplementation in the levels of lipid peroxidation, and antioxidant enzymes in the erythrocyte of rats with alcohol-induced liver injury. Cell Biochem Funct 2004;22:123– 128.
  • [15] Lim E, Hong D, Park J, Joung Y, Darvin P, Kim S, et al. Methylsulfonylmethane Suppresses Breast Cancer Growth by Down-Regulating STAT3 and STAT5b Pathways, PloS one, 2012;7(4): e33361.
  • [16] Kong CS, Bak SS, Rhee SH, Rho CW, Kim NK. Fermentation properties of young radish Kimchi prepared using young radish cultivated in the soil containing sulfur and it’s inhibitory effect on the growth of AGS human gastric adenocarcinoma cells. J Korean Soc Food Sci Nutr 2006;35:158–163.
  • [17] Ha AW, Hong KH, Kim HS, Kim WK. Inorganic sulfur reduces cell proliferation by inhibiting of ErbB2 and ErbB3 protein and mRNA expression in MDA-MB-231 human breast cancer cells. Nutrit Res Pract 2013;7(2):89-95.
  • [18] Georgiou D, Toutountzoglou V, Muir KW, Hadjipavlou-Litina D, Elemes Y. Synthesis of sulfur containing dihydro-pyrrolo derivatives and their biological evaluation as antioxidants. Bio Med Chem 2012;20(17):5103-5109.
  • [19] Pelit E, Oikonomou K, Gul M, Georgiou D, Szafert S, Katsamakas S, Elemes Y. α-Amination and the 5-exo-trig cyclization reaction of sulfur-containing Schiff bases with N-phenyltriazolinedione and their anti-lipid peroxidation activity. Com Ren Chim 2017;20(4):424-434.
  • [20] Hoppe D, Beckmann L. Metallierte Stickstoff-Derivate der Kohlensäure in der organischen Synthese, XIII. Selektive Mono- und Dialkylierung von N-[Bis(alkylthio)-methylen]glycin-ethylestern zum Aufbau von kettenverlängerten und α‐verzweigten α‐ Aminosäureestern, Liebigs Ann Chem 1979;2066-2075.
  • [21] Brand-Williams W, Cuvelier ME, Berset C. Use of a Free Radical Method to Evaluate Antioxidant Activity. LWT—Food Sci Technol 1995;28:25-30.
  • [22] Decker EA, Welch B. Role of Ferritin as a Lipid Oxidation Catalyst in Muscle Food. J. Agric. Food Chem., 1990;38:674-677.
  • [23] Oyaizu M. Studies on Product of Browning Reaction Prepared from Glucose Amine. Jpn J Nutr 1986;44:307-315.
  • [24] Freshney RI. Culture of Animal Cells [6th (sixth) Edition], John Wiley & Sons, Inc., 2010; pp 732.
  • [25] van Meerloo J, Kaspers GJ. Cloos J. Cell sensitivity assays: the MTT assay. Methods in molecular biology (Clifton, N.J.), 2011;731:237-245.
  • [26] Llorent-Martinez EJ, Zengin G, Fernandez-de Cordova, ML, Bender O, Atalay A. Ceylan R., et al. Traditionally Used Lathyrus Species:Phytochemical Composition, Antioxidant Activity, Enzyme Inhibitory Properties,Cytotoxic Effects, and in silico Studies of L. czeczottianus and L.nissolia. Front Pharmacol 2017;8:63.
  • [27] Naydenova ED, Todorov PT, Topashka-Ancheva MN, Momekov G, Yordanova TZ, Konstantinov SM, et al. Novel N (phosphonomethyl) glycine derivatives: Design, characterization and biological activity, Eur J Med Chem 2008;43:1199-1205.
  • [28] Matilla B, Mauriz JL, Culebras JM, González-Gallego J, González P. Glycine: a cell-protecting anti-oxidant nutrient, Nutr Hosp 2002;17(1):2-9.
  • [29] Ladas EJ, Jacobson JS, Kennedy DD, Teel K, Fleischauer A. Kelly KM. Antioxidants and cancer therapy: a systematic review. J Clin Oncol 2004;1;22(3):517-28.
APA Mesci S, Gul M, Yildirim T (2021). Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines. , 81 - 90. 10.33808/clinexphealthsci.787954
Chicago Mesci Seda,Gul Melek,Yildirim Tuba Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines. (2021): 81 - 90. 10.33808/clinexphealthsci.787954
MLA Mesci Seda,Gul Melek,Yildirim Tuba Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines. , 2021, ss.81 - 90. 10.33808/clinexphealthsci.787954
AMA Mesci S,Gul M,Yildirim T Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines. . 2021; 81 - 90. 10.33808/clinexphealthsci.787954
Vancouver Mesci S,Gul M,Yildirim T Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines. . 2021; 81 - 90. 10.33808/clinexphealthsci.787954
IEEE Mesci S,Gul M,Yildirim T "Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines." , ss.81 - 90, 2021. 10.33808/clinexphealthsci.787954
ISNAD Mesci, Seda vd. "Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines". (2021), 81-90. https://doi.org/10.33808/clinexphealthsci.787954
APA Mesci S, Gul M, Yildirim T (2021). Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines. Clinical and Experimental Health Sciences, 11(1), 81 - 90. 10.33808/clinexphealthsci.787954
Chicago Mesci Seda,Gul Melek,Yildirim Tuba Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines. Clinical and Experimental Health Sciences 11, no.1 (2021): 81 - 90. 10.33808/clinexphealthsci.787954
MLA Mesci Seda,Gul Melek,Yildirim Tuba Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines. Clinical and Experimental Health Sciences, vol.11, no.1, 2021, ss.81 - 90. 10.33808/clinexphealthsci.787954
AMA Mesci S,Gul M,Yildirim T Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines. Clinical and Experimental Health Sciences. 2021; 11(1): 81 - 90. 10.33808/clinexphealthsci.787954
Vancouver Mesci S,Gul M,Yildirim T Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines. Clinical and Experimental Health Sciences. 2021; 11(1): 81 - 90. 10.33808/clinexphealthsci.787954
IEEE Mesci S,Gul M,Yildirim T "Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines." Clinical and Experimental Health Sciences, 11, ss.81 - 90, 2021. 10.33808/clinexphealthsci.787954
ISNAD Mesci, Seda vd. "Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines". Clinical and Experimental Health Sciences 11/1 (2021), 81-90. https://doi.org/10.33808/clinexphealthsci.787954
Sayfa Oluşturulma Tarihi
30-04-2024 8:1

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