Yıl: 2021 Cilt: 51 Sayı: 4 Sayfa Aralığı: 2081 - 2086 Metin Dili: İngilizce DOI: 10.3906/sag-2010-245 İndeks Tarihi: 14-01-2022

Circulating miR-29c-3p is downregulated in patients with acromegaly

Öz:
Background/aim: miRNAs control various biological functions, such as cell proliferation, differentiation, signaling pathways, apoptosis and metabolism. Recently, it has been shown that there is a relationship between changes in miRNA expression and the development of acromegaly. Studies are needed to identify new disease-specific miRNAs. The aim of the current study is to evaluate plasma miR-29c-3p, miR-31-5p and miR-18a-5p steady-state levels in acromegaly. Another aim is to investigate whether there is a difference in the levels of these miRNAs in patients with inadequate control and controlled acromegaly with somatostatin analog (SSA) therapy. These miRNAs targeting the IGF-1 gene were determined by in silico estimation. Materials and methods: The study included 30 healthy controls (HC) and 20 patients with acromegaly. Anterior pituitary functions and disease activities of patients with acromegaly were evaluated at the time of study. The miR-29c-3p, miR-31-5p and miR-18a-5p levels were measured using quantitative real-time PCR (RT-qPCR). Results: The expression level of miR-29c-3p was significantly lower in patients with acromegaly compared to the HC group (p < 0.001). This downregulation was more pronounced in patients with inadequately controlled acromegaly than in patients with acromegaly controlled with somatostatin analogues (SSA) therapy (p = 0.016). Univariate logistic regression analysis results showed that down regulation of miR-29c-3p expression increases the risk of developing acromegaly [OR (95% Cl) = 1.605 (1.142-2.257), p = 0.006]. There was no significant difference between the groups in terms of miR-31-5p and miR-18a-5p expression levels (p = 0.375 and p = 0.649, respectively). Conclusion: Plasma miR-29c-3p expression level is downregulated in patients with acromegaly, and this is more pronounced in patients with inadequate control.Key words: Acromegaly, miR-29c-3p, miR-31-5p, miR-18a-5p
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  • 1. Akirov A, Asa SL, Amer L, Shimon I, Ezzat S. The clinicopathological spectrum of acromegaly. Journal of Clinical Medicine 2019; 8 (11): 1962. doi: 10.3390/jcm8111962
  • 2. Feng Y, Mao ZG, Wang X, Du Q, Jian M et al. MicroRNAs and target genes in pituitary adenomas. Hormone and Metabolic Research 2018; 50 (3): 179-192. doi: 10.1055/a-0585-7410
  • 3. Mao ZG, He DS, Zhou J, Yao B, Xiao WW et al. Differential expression of microRNAs in GH-secreting pituitary adenomas. Diagnostic Pathology 2010; 5: 79. doi: 10.1186/1746-1596-5-79
  • 4. Di Ieva A, Butz H, Niamah M, Rotondo F, De Rosa S et al. MicroRNAs as biomarkers in pituitary tumors. Neurosurgery 2014; 75 (2): 181-189. doi: 10.1227/NEU.0000000000000369
  • 5. Katznelson L, Laws ER Jr, Melmed S, Molitch ME, Murad MH et al. Acromegaly: an endocrine society clinical practice guideline.The Journal of Clinical Endocrinology & Metabolism 2014; 99 (11): 3933-3951. doi: 10.1210/jc.2014-2700
  • 6. Shi X, Tao B, He H, Sun Q, Fan C et al. MicroRNAs-based network: a novel therapeutic agent in pituitary adenoma. Medical Hypotheses 2012; 78 (3): 380-384. doi: 10.1016/j. mehy.2011.12.001
  • 7. Fang R, Huang Y, Xie J, Zhang J, Ji X. Downregulation of miR29c-3p is associated with a poor prognosis in patients with laryngeal squamous cell carcinoma. Diagnostic Pathology 2019; 14 (1): 109. doi: 10.1186/s13000-019-0893-2
  • 8. Chen G, Zhou T, Li Y, Yu Z, Sun L. p53 target miR-29c-3p suppresses colon cancer cell invasion and migration through inhibition of PHLDB2. Biochemical and Biophysical Research Communications 2017; 487 (1): 90-95. doi: 10.1016/j. bbrc.2017.04.023
  • 9. Wu H, Zhang W, Wu Z, Liu Y, Shi Y et al. miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma. Cell Death & Disease 2019; 10 (2): 48. doi: 10.1038/s41419-018-1281-7
  • 10. Yan HJ, Ma JY, Wang L, Gu W. Expression and significance of circulating microRNA-31 in lung cancer patients. Medical Science Monitor 2015; 21: 722-726. doi: 10.12659/MSM.893213
  • 11. Shen K, Cao Z, Zhu R, You L, Zhang T. The dual functional role of MicroRNA-18a (miR-18a) in cancer development. Clinical and Translational Medicine 2019; 8 (1): 32. doi: 10.1186/ s40169-019-0250-9
  • 12. Humphreys KJ, McKinnon RA, Michael MZ. miR-18a inhibits CDC42 and plays a tumour suppressor role in colorectal cancer cells. PLoS One 2014; 9 (11): e112288. doi: 10.1371/journal. pone.0112288
  • 13. Liang C, Zhang X, Wang HM, Liu XM, Zhang XJ et al. MicroRNA-18a-5p functions as an oncogene by directly targeting IRF2 in lung cancer. Cell Death & Disease 2017; 8 (5) :e2764. doi: 10.1038/cddis.2017.145
  • 14. Giustina A, Chanson P, Bronstein MD, Klibanski A, Lamberts S et al. A consensus on criteria for cure of acromegaly. The Journal of Clinical Endocrinology & Metabolism 2010; 95 (7): 3141-3148. doi: 10.1210/jc.2009-2670
  • 15. Valassi E, García-Giralt N, Malouf J, Crespo I, Llauger J et al. Circulating miR-103a-3p and miR-660-5p are associated with bone parameters in patients with controlled acromegaly. Endocrine Connections 2019; 8 (1): 39-49. doi: 10.1530/EC18-0482
  • 16. Zhao S, Li J, Feng J, Li Z, Liu Q et al. Identification of Serum miRNA-423-5p expression signature in somatotroph adenomas. International Journal of Endocrinology 2019. doi: 10.1155/2019/8516858
  • 17. Hudcova K, Raudenska M, Gumulec J, Binkova H, Horakova Z et al. Expression profiles of miR-29c, miR-200b and miR-375 in tumour and tumour-adjacent tissues of head and neck cancers. Tumor Biology 2016; 37 (9): 12627-12633. doi: 10.1007/ s13277-016-5147-2
  • 18. Bates PR, Carson MN, Trainer PJ, Wass JA, UK National Acromegaly Register Study Group (UKAR-2). Wide variation in surgical outcomes for acromegaly in the UK. Clinical Endocrinology 2008; 68 (1): 136-142. doi: 10.1111/j.1365- 2265.2007.03012.x
  • 19. Melmed S, Bronstein MD, Chanson P, Klibanski A, Casanueva FF et al. A Consensus Statement on acromegaly therapeutic outcomes. Nature Reviews Endocrinology 2018; 14 (9): 552- 561. doi: 10.1038/s41574-018-0058-5
  • 20. Paragliola RM, Corsello SM, Salvatori R. Somatostatin receptor ligands in acromegaly: clinical response and factors predicting resistance. Pituitary 2017; 20 (1): 109-115. doi: 10.1007/ s11102-016-0768-4
  • 21. Ben-Shlomo A, Melmed S. Somatostatin agonists for treatment of acromegaly. Molecular and Cellular Endocrinology 2008; 286 (1-2): 192-198. doi: 10.1016/j.mce.2007.11.024
  • 22. Capatina C, Wass JA. 60 years of neuroendocrınology: Acromegaly. Journal of Endocrinology 2015; 226 (2): T141-T160. doi: 10.1530/JOE-15-0109
APA Korkmaz H, HEKIMLER OZTÜRK K, Torus B (2021). Circulating miR-29c-3p is downregulated in patients with acromegaly. , 2081 - 2086. 10.3906/sag-2010-245
Chicago Korkmaz Hakan,HEKIMLER OZTÜRK KUYAS,Torus Bora Circulating miR-29c-3p is downregulated in patients with acromegaly. (2021): 2081 - 2086. 10.3906/sag-2010-245
MLA Korkmaz Hakan,HEKIMLER OZTÜRK KUYAS,Torus Bora Circulating miR-29c-3p is downregulated in patients with acromegaly. , 2021, ss.2081 - 2086. 10.3906/sag-2010-245
AMA Korkmaz H,HEKIMLER OZTÜRK K,Torus B Circulating miR-29c-3p is downregulated in patients with acromegaly. . 2021; 2081 - 2086. 10.3906/sag-2010-245
Vancouver Korkmaz H,HEKIMLER OZTÜRK K,Torus B Circulating miR-29c-3p is downregulated in patients with acromegaly. . 2021; 2081 - 2086. 10.3906/sag-2010-245
IEEE Korkmaz H,HEKIMLER OZTÜRK K,Torus B "Circulating miR-29c-3p is downregulated in patients with acromegaly." , ss.2081 - 2086, 2021. 10.3906/sag-2010-245
ISNAD Korkmaz, Hakan vd. "Circulating miR-29c-3p is downregulated in patients with acromegaly". (2021), 2081-2086. https://doi.org/10.3906/sag-2010-245
APA Korkmaz H, HEKIMLER OZTÜRK K, Torus B (2021). Circulating miR-29c-3p is downregulated in patients with acromegaly. Turkish Journal of Medical Sciences, 51(4), 2081 - 2086. 10.3906/sag-2010-245
Chicago Korkmaz Hakan,HEKIMLER OZTÜRK KUYAS,Torus Bora Circulating miR-29c-3p is downregulated in patients with acromegaly. Turkish Journal of Medical Sciences 51, no.4 (2021): 2081 - 2086. 10.3906/sag-2010-245
MLA Korkmaz Hakan,HEKIMLER OZTÜRK KUYAS,Torus Bora Circulating miR-29c-3p is downregulated in patients with acromegaly. Turkish Journal of Medical Sciences, vol.51, no.4, 2021, ss.2081 - 2086. 10.3906/sag-2010-245
AMA Korkmaz H,HEKIMLER OZTÜRK K,Torus B Circulating miR-29c-3p is downregulated in patients with acromegaly. Turkish Journal of Medical Sciences. 2021; 51(4): 2081 - 2086. 10.3906/sag-2010-245
Vancouver Korkmaz H,HEKIMLER OZTÜRK K,Torus B Circulating miR-29c-3p is downregulated in patients with acromegaly. Turkish Journal of Medical Sciences. 2021; 51(4): 2081 - 2086. 10.3906/sag-2010-245
IEEE Korkmaz H,HEKIMLER OZTÜRK K,Torus B "Circulating miR-29c-3p is downregulated in patients with acromegaly." Turkish Journal of Medical Sciences, 51, ss.2081 - 2086, 2021. 10.3906/sag-2010-245
ISNAD Korkmaz, Hakan vd. "Circulating miR-29c-3p is downregulated in patients with acromegaly". Turkish Journal of Medical Sciences 51/4 (2021), 2081-2086. https://doi.org/10.3906/sag-2010-245