Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience

Öz:

Background/aim: Glanzmann thrombasthenia (GT) is a rare autosomal recessively inherited bleeding disorder characterized by the quantitative (type 1 and type 2) or qualitative (type 3) deficiency in platelet membrane glycoprotein (GP) IIb/IIIa (CD41a/CD61) fibrinogen receptors. In type 1, 2, and 3, CD41a/CD61 expression is 5%, 5%–20% and above 20%, respectively. In this study, diagnosis of GT was confirmed and subgroups were identified in 32 Turkish patients by flow cytometry analysis. Materials and methods: CD41a/CD61 expression levels in platelet-rich plasma (PRP) obtained from peripheral venous EDTA blood samples were analyzed with a BD FACSCanto II flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA). GT subgroup analysis was performed by counting 50,000 events in the BD FACSDiva Software v6.1.3 program of the instrument. Results: In the present study, in blood samples of 32 patients from 23 families with GT and 22 healthy controls, co-expression levels of CD41a and CD61 in PRP was analyzed. 12 out of 23 families were consistent with type 1 GT (52.2%), 4 were consistent with type 2 GT (17.4%), and 7 were consistent with type 3 GT (30.4%). Conclusion: Especially due to consanguineous marriages, GT with various glycoprotein levels may be detected. As a result of the flow cytometry analysis of the present study with the highest GT patient population in Turkey, type 1 GT patients were the most common subgroup. In the determination of the GT subgroups; especially in the detection of type 3 GT, flow cytometry is the most sensitive glycoprotein analysis method. In addition to light transmission aggregometry, CD41a/CD61 study by flow cytometer confirms diagnosis when mutation analysis cannot be performed.Key words: Glanzmann thrombasthenia, glycoprotein IIb/IIIa, flow cytometry, platelet-rich plasma

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APA	SARAYMEN B, MUHTAROĞLU S, koker M, Sarper N, zengin e, albayrak c, Albayrak D, Zulfikar B, Koc B, Bentli E, YILMAZ S, Cetin A, Eser B, ÇETİN M (2021). Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience. , 2135 - 2141. 10.3906/sag-2006-107
Chicago	SARAYMEN BERKAY,MUHTAROĞLU Sabahattin,koker Mustafa Yavuz,Sarper Nazan,zengin emine,albayrak canan,Albayrak Davut,Zulfikar Bulent,Koc Basak,Bentli Esma,YILMAZ SEMİH,Cetin Aysun,Eser Bülent,ÇETİN Mustafa Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience. (2021): 2135 - 2141. 10.3906/sag-2006-107
MLA	SARAYMEN BERKAY,MUHTAROĞLU Sabahattin,koker Mustafa Yavuz,Sarper Nazan,zengin emine,albayrak canan,Albayrak Davut,Zulfikar Bulent,Koc Basak,Bentli Esma,YILMAZ SEMİH,Cetin Aysun,Eser Bülent,ÇETİN Mustafa Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience. , 2021, ss.2135 - 2141. 10.3906/sag-2006-107
AMA	SARAYMEN B,MUHTAROĞLU S,koker M,Sarper N,zengin e,albayrak c,Albayrak D,Zulfikar B,Koc B,Bentli E,YILMAZ S,Cetin A,Eser B,ÇETİN M Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience. . 2021; 2135 - 2141. 10.3906/sag-2006-107
Vancouver	SARAYMEN B,MUHTAROĞLU S,koker M,Sarper N,zengin e,albayrak c,Albayrak D,Zulfikar B,Koc B,Bentli E,YILMAZ S,Cetin A,Eser B,ÇETİN M Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience. . 2021; 2135 - 2141. 10.3906/sag-2006-107
IEEE	SARAYMEN B,MUHTAROĞLU S,koker M,Sarper N,zengin e,albayrak c,Albayrak D,Zulfikar B,Koc B,Bentli E,YILMAZ S,Cetin A,Eser B,ÇETİN M "Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience." , ss.2135 - 2141, 2021. 10.3906/sag-2006-107
ISNAD	SARAYMEN, BERKAY vd. "Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience". (2021), 2135-2141. https://doi.org/10.3906/sag-2006-107

APA	SARAYMEN B, MUHTAROĞLU S, koker M, Sarper N, zengin e, albayrak c, Albayrak D, Zulfikar B, Koc B, Bentli E, YILMAZ S, Cetin A, Eser B, ÇETİN M (2021). Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience. Turkish Journal of Medical Sciences, 51(4), 2135 - 2141. 10.3906/sag-2006-107
Chicago	SARAYMEN BERKAY,MUHTAROĞLU Sabahattin,koker Mustafa Yavuz,Sarper Nazan,zengin emine,albayrak canan,Albayrak Davut,Zulfikar Bulent,Koc Basak,Bentli Esma,YILMAZ SEMİH,Cetin Aysun,Eser Bülent,ÇETİN Mustafa Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience. Turkish Journal of Medical Sciences 51, no.4 (2021): 2135 - 2141. 10.3906/sag-2006-107
MLA	SARAYMEN BERKAY,MUHTAROĞLU Sabahattin,koker Mustafa Yavuz,Sarper Nazan,zengin emine,albayrak canan,Albayrak Davut,Zulfikar Bulent,Koc Basak,Bentli Esma,YILMAZ SEMİH,Cetin Aysun,Eser Bülent,ÇETİN Mustafa Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience. Turkish Journal of Medical Sciences, vol.51, no.4, 2021, ss.2135 - 2141. 10.3906/sag-2006-107
AMA	SARAYMEN B,MUHTAROĞLU S,koker M,Sarper N,zengin e,albayrak c,Albayrak D,Zulfikar B,Koc B,Bentli E,YILMAZ S,Cetin A,Eser B,ÇETİN M Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience. Turkish Journal of Medical Sciences. 2021; 51(4): 2135 - 2141. 10.3906/sag-2006-107
Vancouver	SARAYMEN B,MUHTAROĞLU S,koker M,Sarper N,zengin e,albayrak c,Albayrak D,Zulfikar B,Koc B,Bentli E,YILMAZ S,Cetin A,Eser B,ÇETİN M Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience. Turkish Journal of Medical Sciences. 2021; 51(4): 2135 - 2141. 10.3906/sag-2006-107
IEEE	SARAYMEN B,MUHTAROĞLU S,koker M,Sarper N,zengin e,albayrak c,Albayrak D,Zulfikar B,Koc B,Bentli E,YILMAZ S,Cetin A,Eser B,ÇETİN M "Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience." Turkish Journal of Medical Sciences, 51, ss.2135 - 2141, 2021. 10.3906/sag-2006-107
ISNAD	SARAYMEN, BERKAY vd. "Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience". Turkish Journal of Medical Sciences 51/4 (2021), 2135-2141. https://doi.org/10.3906/sag-2006-107