Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study

BAYDENİZ, Soner Baydeniz; SAYAR, ILYAS; Bozkurt, Aliseydi; GÜRBÜZEL, MEHMET; ARSLAN, YUSUF KEMAL

doi:10.5455/annalsmedres.2021.01.112

Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study

Soner Baydeniz, (Biyoloji Bölümü, Fen Bilimleri Enstitüsü, Erzincan Binali Yıldırım Üniversitesi, Erzincan, Türkiye)

Mehmet GÜRBÜZEL, (Erzincan Binali Yıldırım Üniversitesi Tıp Fakültesi Tıbbi Biyoloji Anabilim Dalı, Erzincan, Türkiye)

Ilyas Sayar, (Erzincan Binali Yıldırım Üniversitesi Tıp Fakültesi Patoloji Anabilim Dalı, Erzincan, Türkiye)

Aliseydi Bozkurt, (Erzincan Binali Yıldırım Üniversitesi Tıp Fakültesi Üroloji Anabilim Dalı, Erzincan, Türkiye)

Yusuf Kemal Arslan (Erzincan Binali Yıldırım Üniversitesi Tıp Fakültesi Biyoistatistik Anabilim Dalı, Erzincan, Türkiye)

Annals of Medical Research

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Yıl: 2021 Cilt: 28 Sayı: 10 Sayfa Aralığı: 1926 - 1932 Metin Dili: İngilizce DOI: 10.5455/annalsmedres.2021.01.112 İndeks Tarihi: 18-01-2022

Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study

Öz:

Aim: The level of free DNA is used as a cancer biomarker due to having several advantages related to lower cost, easy applicability, and rapid results compared to invasive methods. Materials and Methods: This study investigated whether or not free DNA functions as intended when stored. The free DNA isolated for this purpose was kept at –80 degrees for four years. Then, the level of free DNA was measured in patients with bladder cancer by both photometric and fluorometric methods, and it was investigated whether this biomarker was effective in determining Single Nucleotide Polymorphisms (SNP). The gene names and the reference numbers of the locations where polymorphism was expected were as follows: CCAT2 (rs6983267), HRAS (rs12628), and RET (rs1799939). Results: According to the results, having the T allele at rs12628 increases the risk of disease by 2.6 times (OR: 2.60; 95% CI: 1.10– 6.10) and having the G allele at rs1799939 increases the risk of disease 2.7 times (OR: 2.70; 95% CI: 1.12–6.69). Conclusions: The general findings showed that fluorometric measurements were more advantageous when considering the sensitivity of free DNA measurements. It was also concluded that, if treated rapidly and conserved in appropriate laboratory conditions, free DNA can be effectively used in SNP studies. This study provides important data to demonstrate the quality of free DNA.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BAYDENİZ S, GÜRBÜZEL M, SAYAR I, Bozkurt A, ARSLAN Y (2021). Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study. , 1926 - 1932. 10.5455/annalsmedres.2021.01.112
Chicago	BAYDENİZ Soner Baydeniz,GÜRBÜZEL MEHMET,SAYAR ILYAS,Bozkurt Aliseydi,ARSLAN YUSUF KEMAL Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study. (2021): 1926 - 1932. 10.5455/annalsmedres.2021.01.112
MLA	BAYDENİZ Soner Baydeniz,GÜRBÜZEL MEHMET,SAYAR ILYAS,Bozkurt Aliseydi,ARSLAN YUSUF KEMAL Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study. , 2021, ss.1926 - 1932. 10.5455/annalsmedres.2021.01.112
AMA	BAYDENİZ S,GÜRBÜZEL M,SAYAR I,Bozkurt A,ARSLAN Y Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study. . 2021; 1926 - 1932. 10.5455/annalsmedres.2021.01.112
Vancouver	BAYDENİZ S,GÜRBÜZEL M,SAYAR I,Bozkurt A,ARSLAN Y Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study. . 2021; 1926 - 1932. 10.5455/annalsmedres.2021.01.112
IEEE	BAYDENİZ S,GÜRBÜZEL M,SAYAR I,Bozkurt A,ARSLAN Y "Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study." , ss.1926 - 1932, 2021. 10.5455/annalsmedres.2021.01.112
ISNAD	BAYDENİZ, Soner Baydeniz vd. "Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study". (2021), 1926-1932. https://doi.org/10.5455/annalsmedres.2021.01.112

APA	BAYDENİZ S, GÜRBÜZEL M, SAYAR I, Bozkurt A, ARSLAN Y (2021). Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study. Annals of Medical Research, 28(10), 1926 - 1932. 10.5455/annalsmedres.2021.01.112
Chicago	BAYDENİZ Soner Baydeniz,GÜRBÜZEL MEHMET,SAYAR ILYAS,Bozkurt Aliseydi,ARSLAN YUSUF KEMAL Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study. Annals of Medical Research 28, no.10 (2021): 1926 - 1932. 10.5455/annalsmedres.2021.01.112
MLA	BAYDENİZ Soner Baydeniz,GÜRBÜZEL MEHMET,SAYAR ILYAS,Bozkurt Aliseydi,ARSLAN YUSUF KEMAL Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study. Annals of Medical Research, vol.28, no.10, 2021, ss.1926 - 1932. 10.5455/annalsmedres.2021.01.112
AMA	BAYDENİZ S,GÜRBÜZEL M,SAYAR I,Bozkurt A,ARSLAN Y Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study. Annals of Medical Research. 2021; 28(10): 1926 - 1932. 10.5455/annalsmedres.2021.01.112
Vancouver	BAYDENİZ S,GÜRBÜZEL M,SAYAR I,Bozkurt A,ARSLAN Y Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study. Annals of Medical Research. 2021; 28(10): 1926 - 1932. 10.5455/annalsmedres.2021.01.112
IEEE	BAYDENİZ S,GÜRBÜZEL M,SAYAR I,Bozkurt A,ARSLAN Y "Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study." Annals of Medical Research, 28, ss.1926 - 1932, 2021. 10.5455/annalsmedres.2021.01.112
ISNAD	BAYDENİZ, Soner Baydeniz vd. "Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study". Annals of Medical Research 28/10 (2021), 1926-1932. https://doi.org/10.5455/annalsmedres.2021.01.112