Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury

Saribas, Gulistan Sanem; Karahan, Sevilay; Bayır, Ömer; Alpaslan Pinarli, Ferda; Korkmaz, Mehmet Hakan; Saylam, GuLESER; OCAL, BULENT; ÇADALLI TATAR, EMEL; keseroglu, kemal; Karagöz, Tuğba; Özoğul, Candan

doi:10.3906/sag-2004-3

Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury

Ömer BAYIR, (Sağlık Bilimleri Üniversitesi, Dışkapı Yıldırım Beyazıt Eğitim ve Araştırma Hastanesi, Kulak Burun Boğaz Anabilim Dalı, Ankara, Türkiye)

Tuğba KARAGÖZ, (Kaman Devlet Hastanesi, Kulak Burun Boğaz ve Baş Boyun Cerrahisi Anabilim Dalı, Kırşehir, Türkiye)

Ferda ALPASLAN PINARLI, (Myogen Laboratuvarı ve Sağlık Hizmetleri, Ankara, Türkiye)

Gülistan Sanem SARIBAŞ, (Ahi Evran Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Kırşehir, Türkiye)

Candan ÖZOĞUL, (Girne Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Girne, KKTC)

Kemal KESEROĞLU, (Sağlık Bilimleri Üniversitesi, Dışkapı Yıldırım Beyazıt Eğitim ve Araştırma Hastanesi, Kulak Burun Boğaz Anabilim Dalı, Ankara, Türkiye)

Güleser SAYLAM, (Sağlık Bilimleri Üniversitesi, Dışkapı Yıldırım Beyazıt Eğitim ve Araştırma Hastanesi, Kulak Burun Boğaz Anabilim Dalı, Ankara, Türkiye)

Emel ÇADALLI TATAR, (Sağlık Bilimleri Üniversitesi, Dışkapı Yıldırım Beyazıt Eğitim ve Araştırma Hastanesi, Kulak Burun Boğaz Anabilim Dalı, Ankara, Türkiye)

Sevilay KARAHAN, (Hacettepe Üniversitesi, Tıp Fakültesi, Biyoistatistik Anabilim Dalı, Ankara, Türkiye)

Bülent ÖCAL, (Sağlık Bilimleri Üniversitesi, Dışkapı Yıldırım Beyazıt Eğitim ve Araştırma Hastanesi, Kulak Burun Boğaz Anabilim Dalı, Ankara, Türkiye)

Mehmet Hakan KORKMAZ, (Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Kulak Burun Boğaz Anabilim Dalı, Ankara, Türkiye)

Diğer Yazarlar

Turkish Journal of Medical Sciences

0 0

Yıl: 2021 Cilt: 51 Sayı: 3 Sayfa Aralığı: 1481 - 1490 Metin Dili: İngilizce DOI: 10.3906/sag-2004-3 İndeks Tarihi: 20-01-2022

Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury

Öz:

Background/aim: To evaluate the clinical and histopathological effects of fetal brain tissue derived mesenchymal stem cells (FBTMSC) and fibrin glue (FG) on the facial nerve (FN) regeneration in rats with traumatic FN injury. Materials and methods: Twenty-eight Sprague Dawley rats were included in the study and divided into 4 groups. Traumatic FN injury (FP) was created by a surgical clamp compression to the main trunk of left FN in all groups. In the control group (group 1) no treatment was applied, in group 2 (FBTMSC group) 2 × 106 FBTMSC was injected, in group 3 (FG group) only FG was applied, in group 4 (FBTMSC and FG groups) both FBTMSC and FG were applied to the injured section of the nerve. The FN functions were evaluated clinically, immediately after the procedure and at 3rd, 5th, and 8th weeks postoperatively. The FNs of all subjects were excised after the 8th week; then the rats were sacrificed. The presence of stem cells in the injured zone was assessed using bromo-deoxyuridine (BrdU), and apoptosis was determined by the TUNEL method. Results: After the damage, total FP was observed in all subjects. Statistically significant functional improvement was observed in group 4 compared to all other groups (P < 0.005). TUNEL-positive cell count was statistically significantly higher in the control group than the other groups (P < 0.001). TUNEL-positive cell count was statistically significantly lower in group 4 than the other groups. The proportion of BrdU-stained cells in group 4 (5%) was higher than group 2 (2%). Conclusion: Clinically and histopathologically FBTMSC applied with FG may play a promising role as a regenerative treatment in posttraumatic FP.Key words: Facial nerve, facial nerve palsy, trauma, stem cell implantation, fetal brain tissue derived mesenchymal stem cells

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Bayır Ö, Karagöz T, Alpaslan Pinarli F, Saribas G, Özoğul C, keseroglu k, Saylam G, ÇADALLI TATAR E, Karahan S, OCAL B, Korkmaz M (2021). Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury. , 1481 - 1490. 10.3906/sag-2004-3
Chicago	Bayır Ömer,Karagöz Tuğba,Alpaslan Pinarli Ferda,Saribas Gulistan Sanem,Özoğul Candan,keseroglu kemal,Saylam GuLESER,ÇADALLI TATAR EMEL,Karahan Sevilay,OCAL BULENT,Korkmaz Mehmet Hakan Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury. (2021): 1481 - 1490. 10.3906/sag-2004-3
MLA	Bayır Ömer,Karagöz Tuğba,Alpaslan Pinarli Ferda,Saribas Gulistan Sanem,Özoğul Candan,keseroglu kemal,Saylam GuLESER,ÇADALLI TATAR EMEL,Karahan Sevilay,OCAL BULENT,Korkmaz Mehmet Hakan Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury. , 2021, ss.1481 - 1490. 10.3906/sag-2004-3
AMA	Bayır Ö,Karagöz T,Alpaslan Pinarli F,Saribas G,Özoğul C,keseroglu k,Saylam G,ÇADALLI TATAR E,Karahan S,OCAL B,Korkmaz M Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury. . 2021; 1481 - 1490. 10.3906/sag-2004-3
Vancouver	Bayır Ö,Karagöz T,Alpaslan Pinarli F,Saribas G,Özoğul C,keseroglu k,Saylam G,ÇADALLI TATAR E,Karahan S,OCAL B,Korkmaz M Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury. . 2021; 1481 - 1490. 10.3906/sag-2004-3
IEEE	Bayır Ö,Karagöz T,Alpaslan Pinarli F,Saribas G,Özoğul C,keseroglu k,Saylam G,ÇADALLI TATAR E,Karahan S,OCAL B,Korkmaz M "Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury." , ss.1481 - 1490, 2021. 10.3906/sag-2004-3
ISNAD	Bayır, Ömer vd. "Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury". (2021), 1481-1490. https://doi.org/10.3906/sag-2004-3

APA	Bayır Ö, Karagöz T, Alpaslan Pinarli F, Saribas G, Özoğul C, keseroglu k, Saylam G, ÇADALLI TATAR E, Karahan S, OCAL B, Korkmaz M (2021). Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury. Turkish Journal of Medical Sciences, 51(3), 1481 - 1490. 10.3906/sag-2004-3
Chicago	Bayır Ömer,Karagöz Tuğba,Alpaslan Pinarli Ferda,Saribas Gulistan Sanem,Özoğul Candan,keseroglu kemal,Saylam GuLESER,ÇADALLI TATAR EMEL,Karahan Sevilay,OCAL BULENT,Korkmaz Mehmet Hakan Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury. Turkish Journal of Medical Sciences 51, no.3 (2021): 1481 - 1490. 10.3906/sag-2004-3
MLA	Bayır Ömer,Karagöz Tuğba,Alpaslan Pinarli Ferda,Saribas Gulistan Sanem,Özoğul Candan,keseroglu kemal,Saylam GuLESER,ÇADALLI TATAR EMEL,Karahan Sevilay,OCAL BULENT,Korkmaz Mehmet Hakan Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury. Turkish Journal of Medical Sciences, vol.51, no.3, 2021, ss.1481 - 1490. 10.3906/sag-2004-3
AMA	Bayır Ö,Karagöz T,Alpaslan Pinarli F,Saribas G,Özoğul C,keseroglu k,Saylam G,ÇADALLI TATAR E,Karahan S,OCAL B,Korkmaz M Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury. Turkish Journal of Medical Sciences. 2021; 51(3): 1481 - 1490. 10.3906/sag-2004-3
Vancouver	Bayır Ö,Karagöz T,Alpaslan Pinarli F,Saribas G,Özoğul C,keseroglu k,Saylam G,ÇADALLI TATAR E,Karahan S,OCAL B,Korkmaz M Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury. Turkish Journal of Medical Sciences. 2021; 51(3): 1481 - 1490. 10.3906/sag-2004-3
IEEE	Bayır Ö,Karagöz T,Alpaslan Pinarli F,Saribas G,Özoğul C,keseroglu k,Saylam G,ÇADALLI TATAR E,Karahan S,OCAL B,Korkmaz M "Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury." Turkish Journal of Medical Sciences, 51, ss.1481 - 1490, 2021. 10.3906/sag-2004-3
ISNAD	Bayır, Ömer vd. "Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury". Turkish Journal of Medical Sciences 51/3 (2021), 1481-1490. https://doi.org/10.3906/sag-2004-3