TY  - JOUR
TI  - Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury
AB  - Background/aim: To evaluate the clinical and histopathological effects of fetal brain tissue derived mesenchymal stem cells (FBTMSC) and fibrin glue (FG) on the facial nerve (FN) regeneration in rats with traumatic FN injury. Materials and methods: Twenty-eight Sprague Dawley rats were included in the study and divided into 4 groups. Traumatic FN injury (FP) was created by a surgical clamp compression to the main trunk of left FN in all groups. In the control group (group 1) no treatment was applied, in group 2 (FBTMSC group) 2 × 106 FBTMSC was injected, in group 3 (FG group) only FG was applied, in group 4 (FBTMSC and FG groups) both FBTMSC and FG were applied to the injured section of the nerve. The FN functions were evaluated clinically, immediately after the procedure and at 3rd, 5th, and 8th weeks postoperatively. The FNs of all subjects were excised after the 8th week; then the rats were sacrificed. The presence of stem cells in the injured zone was assessed using bromo-deoxyuridine (BrdU), and apoptosis was determined by the TUNEL method. Results: After the damage, total FP was observed in all subjects. Statistically significant functional improvement was observed in group 4 compared to all other groups (P < 0.005). TUNEL-positive cell count was statistically significantly higher in the control group than the other groups (P < 0.001). TUNEL-positive cell count was statistically significantly lower in group 4 than the other groups. The proportion of BrdU-stained cells in group 4 (5%) was higher than group 2 (2%). Conclusion: Clinically and histopathologically FBTMSC applied with FG may play a promising role as a regenerative treatment in posttraumatic FP.Key words: Facial nerve, facial nerve palsy, trauma, stem cell implantation, fetal brain tissue derived mesenchymal stem cells
AU  - Saribas, Gulistan Sanem
AU  - Karahan, Sevilay
AU  - Bayır, Ömer
AU  - Alpaslan Pinarli, Ferda
AU  - Korkmaz, Mehmet Hakan
AU  - Saylam, GuLESER
AU  - OCAL, BULENT
AU  - ÇADALLI TATAR, EMEL
AU  - keseroglu, kemal
AU  - Karagöz, Tuğba
AU  - Özoğul, Candan
DO  - 10.3906/sag-2004-3
PY  - 2021
JO  - Turkish Journal of Medical Sciences
VL  - 51
IS  - 3
SN  - 1300-0144
SP  - 1481
EP  - 1490
DB  - TRDizin
UR  - http://search/yayin/detay/483233
ER  -