Yıl: 2021 Cilt: 51 Sayı: 3 Sayfa Aralığı: 1530 - 1542 Metin Dili: İngilizce DOI: 10.3906/sag-2003-10 İndeks Tarihi: 20-01-2022

Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide

Öz:
Backround/aim: Cyclophosphamide (CP) is a drug used for treatment of many malignant diseases. However, it can cause serious side effects such as hemorrhagic cystitis and male infertility. Hydrogen sulfide (H2 S) is a gaseous mediator and is suggested to have antioxidant, antiinflammatory, and antiapoptotic effects. In this study, dose-dependent effects of H2 S donor sodium hydrosulfide (NaHS) on cyclophosphamide-induced hemorrhagic cystitis and testicular dysfunction were investigated in rats. Material and methods: Rats were divided into 5 groups (n = 8): control, CP, NaHS25 μmol/kg, NaHS50 μmol/kg, and NaHS100 μmol/ kg. After treatment for 7 days intraperitoneally (ip), a single ip dose of CP 200 mg/kg was given on the 8th day. Then, treatment was continued for 7 days. In bladder and testicular tissues, IL 6, IL 10, cGMP, NO, H2 S, FSH, LH, and testosterone levels were measured by ELISA. Histopathological examination with H&E staining, as well as immunohistochemical staining for acrolein in bladder and caspase-3 and APAF-1 in testis were performed. Results: NaHS prevented the increased IL 6 and IL 10 values induced by CP as well as prevented the decrease in cGMP values associated with CP. There was no significant change in FSH values, but the LH value, which increased with CP, decreased with 25, 50, and 100 µmol/kg NaHS. In contrast, testosterone decreased in the CP group and increased in the treatment groups. NaHS was effective in many biochemical and histopathological parameters at 25 and 50 µmol/kg doses, and this effect decreased at 100 µmol/kg dose. Conclusion: H2 S has a protective and therapeutic effect on hemorrhagic cystitis and testicular dysfunction induced by cyclophosphamide. It can be suggested that H2 S is a promising molecule in facilitating cancer treatment.Key words: Cyclophosphamide, hydrogen sulfide, testis, bladder
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APA Özatik F, Özatik O, Tekşen Y, yigitaslan s, ARI N (2021). Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide. , 1530 - 1542. 10.3906/sag-2003-10
Chicago Özatik Fikriye Yasemin,Özatik Orhan,Tekşen Yasemin,yigitaslan semra,ARI Neziha Senem Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide. (2021): 1530 - 1542. 10.3906/sag-2003-10
MLA Özatik Fikriye Yasemin,Özatik Orhan,Tekşen Yasemin,yigitaslan semra,ARI Neziha Senem Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide. , 2021, ss.1530 - 1542. 10.3906/sag-2003-10
AMA Özatik F,Özatik O,Tekşen Y,yigitaslan s,ARI N Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide. . 2021; 1530 - 1542. 10.3906/sag-2003-10
Vancouver Özatik F,Özatik O,Tekşen Y,yigitaslan s,ARI N Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide. . 2021; 1530 - 1542. 10.3906/sag-2003-10
IEEE Özatik F,Özatik O,Tekşen Y,yigitaslan s,ARI N "Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide." , ss.1530 - 1542, 2021. 10.3906/sag-2003-10
ISNAD Özatik, Fikriye Yasemin vd. "Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide". (2021), 1530-1542. https://doi.org/10.3906/sag-2003-10
APA Özatik F, Özatik O, Tekşen Y, yigitaslan s, ARI N (2021). Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide. Turkish Journal of Medical Sciences, 51(3), 1530 - 1542. 10.3906/sag-2003-10
Chicago Özatik Fikriye Yasemin,Özatik Orhan,Tekşen Yasemin,yigitaslan semra,ARI Neziha Senem Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide. Turkish Journal of Medical Sciences 51, no.3 (2021): 1530 - 1542. 10.3906/sag-2003-10
MLA Özatik Fikriye Yasemin,Özatik Orhan,Tekşen Yasemin,yigitaslan semra,ARI Neziha Senem Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide. Turkish Journal of Medical Sciences, vol.51, no.3, 2021, ss.1530 - 1542. 10.3906/sag-2003-10
AMA Özatik F,Özatik O,Tekşen Y,yigitaslan s,ARI N Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide. Turkish Journal of Medical Sciences. 2021; 51(3): 1530 - 1542. 10.3906/sag-2003-10
Vancouver Özatik F,Özatik O,Tekşen Y,yigitaslan s,ARI N Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide. Turkish Journal of Medical Sciences. 2021; 51(3): 1530 - 1542. 10.3906/sag-2003-10
IEEE Özatik F,Özatik O,Tekşen Y,yigitaslan s,ARI N "Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide." Turkish Journal of Medical Sciences, 51, ss.1530 - 1542, 2021. 10.3906/sag-2003-10
ISNAD Özatik, Fikriye Yasemin vd. "Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide". Turkish Journal of Medical Sciences 51/3 (2021), 1530-1542. https://doi.org/10.3906/sag-2003-10