Development of Iridium Based Fluorimetric Method for Determination of Cysteine
Yıl: 2021 Cilt: 49 Sayı: 4 Sayfa Aralığı: 355 - 365 Metin Dili: İngilizce DOI: 10.15671/hjbc.798021 İndeks Tarihi: 21-01-2022
Development of Iridium Based Fluorimetric Method for Determination of Cysteine
Öz: Cysteine (Cys) and homocysteine (Hcy) amino acids that are containing thiol groups play many important roles in bi - ological systems. The plasma level of Hcy is known to be an indicator of some disorders such as cardiovascular and Alzheimer’s disease. Cys deficiency in plasma is associated with liver damage, muscle and fat loss, skin lesions, slowed growth, etc. In this study, iridium-based complexes were chosen for developing a fluorescent-based method for the deter - mination of Cys levels due to their high photoluminescence efficiency. For this aim, the iridium (III) complex was synthesized then, this complex interacted with Cys solutions. It was found that, as the concentration of Cys interacting with the iridium complex, the fluorescence intensity increased. Based on these data, it was thought that the synthesized iridium complex could be used in the fluorimetric determination of Cys level in serum samples.
Anahtar Kelime: Sistein Tayini için İridyum Tabanlı Fluorimetrik Metot Geliştirilmesi
Öz: iyol grupları içeren aminoasitler olarak Sistein (Cys) ve homosistein (Hcy) biyolojik sistemlerde birçok önemli rol oynamak- tadır. HCy’nin plazma seviyesinin, kardiyovasküler ve Alzheimer gibi bazı hastalıkların bir göstergesi olduğu bilinmektedir. Plazmadaki Cys eksikliği karaciğer hasarı, kas ve yağ kaybı, cilt lezyonları, yavaş büyüme vb. Gibi durumlarla ilişkilidir. Bu çalışmada, Cys seviyelerinin belirlenmesi için floresan tabanlı bir metot geliştirilmek üzere yüksek fotolüminesans etkinlik gösteren iridium (III) kompleksleri seçilmiştir. Daha sonra sentezlenen kompleks, Cys çözeltileriyle etkileştirilmiştir. İridyum kompleksiyle etkileşen Cys derişimi arttıkça çözeltinin floresans şiddetinin de arttığı bulunmuştur. Buradan yola çıkarak, sentezlenen iridyum kompleksinin, serum örneklerinde Cys seviyesinin florimetrik tayininde kullanılabileceği düşünülmek - tedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ÜNLÜER Ö (2021). Development of Iridium Based Fluorimetric Method for Determination of Cysteine. , 355 - 365. 10.15671/hjbc.798021 |
| Chicago | ÜNLÜER Özlem Biçen Development of Iridium Based Fluorimetric Method for Determination of Cysteine. (2021): 355 - 365. 10.15671/hjbc.798021 |
| MLA | ÜNLÜER Özlem Biçen Development of Iridium Based Fluorimetric Method for Determination of Cysteine. , 2021, ss.355 - 365. 10.15671/hjbc.798021 |
| AMA | ÜNLÜER Ö Development of Iridium Based Fluorimetric Method for Determination of Cysteine. . 2021; 355 - 365. 10.15671/hjbc.798021 |
| Vancouver | ÜNLÜER Ö Development of Iridium Based Fluorimetric Method for Determination of Cysteine. . 2021; 355 - 365. 10.15671/hjbc.798021 |
| IEEE | ÜNLÜER Ö "Development of Iridium Based Fluorimetric Method for Determination of Cysteine." , ss.355 - 365, 2021. 10.15671/hjbc.798021 |
| ISNAD | ÜNLÜER, Özlem Biçen. "Development of Iridium Based Fluorimetric Method for Determination of Cysteine". (2021), 355-365. https://doi.org/10.15671/hjbc.798021 |
| APA | ÜNLÜER Ö (2021). Development of Iridium Based Fluorimetric Method for Determination of Cysteine. Hacettepe Journal of Biology and Chemistry, 49(4), 355 - 365. 10.15671/hjbc.798021 |
| Chicago | ÜNLÜER Özlem Biçen Development of Iridium Based Fluorimetric Method for Determination of Cysteine. Hacettepe Journal of Biology and Chemistry 49, no.4 (2021): 355 - 365. 10.15671/hjbc.798021 |
| MLA | ÜNLÜER Özlem Biçen Development of Iridium Based Fluorimetric Method for Determination of Cysteine. Hacettepe Journal of Biology and Chemistry, vol.49, no.4, 2021, ss.355 - 365. 10.15671/hjbc.798021 |
| AMA | ÜNLÜER Ö Development of Iridium Based Fluorimetric Method for Determination of Cysteine. Hacettepe Journal of Biology and Chemistry. 2021; 49(4): 355 - 365. 10.15671/hjbc.798021 |
| Vancouver | ÜNLÜER Ö Development of Iridium Based Fluorimetric Method for Determination of Cysteine. Hacettepe Journal of Biology and Chemistry. 2021; 49(4): 355 - 365. 10.15671/hjbc.798021 |
| IEEE | ÜNLÜER Ö "Development of Iridium Based Fluorimetric Method for Determination of Cysteine." Hacettepe Journal of Biology and Chemistry, 49, ss.355 - 365, 2021. 10.15671/hjbc.798021 |
| ISNAD | ÜNLÜER, Özlem Biçen. "Development of Iridium Based Fluorimetric Method for Determination of Cysteine". Hacettepe Journal of Biology and Chemistry 49/4 (2021), 355-365. https://doi.org/10.15671/hjbc.798021 |
