N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis
Yıl: 2021 Cilt: 49 Sayı: 2 Sayfa Aralığı: 147 - 155 Metin Dili: İngilizce DOI: 10.15671/hjbc.717600 İndeks Tarihi: 21-01-2022
N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis
Öz: Nowadays, the use of microalgae species as raw materials in biopharmaceutical production is on the agenda. The reasonbehind this idea is that microalgae are cell factories that are able to efficiently utilize carbon dioxide for the production of numerous biologically active compounds. However, there are several problems that remain to be solved in the production of recombinant protein from microalgaes. One of the critical requirements is to produce a bio-compatible N-glycosylation profile from the secreted recombinant proteins. The knowledge about the glycosylation machinery and N-glycan profiles of microalgae spices are quite limited. In the study, it was aimed to characterize N-glycan profiles of a green microalgae, Chlorella zofingiensis. To achieve this, photoautotrophically grown Chlorella zofingiensis extracts including (glyco-)proteins were enzymatically deglycosylated and labelled with 2-aminobenzoic acid tag. Released N-glycans were purified with a HILIC-based approach and analyzed by MALDI-TOF(/TOF)-MS. The results showed that C. zofingiensis included oligomanno- sidic type N-glycan patterns. In addition, N-glycosylation profiles of C. zofingiensis by MALDI-MS revealed that most of the oligomannosidic N-glycans were phosphorylated.
Anahtar Kelime: Yeşil Mikroalg Türü Chlorella Zofingiensis’in N-glikozliasyon Profili
Öz: Günümüzde, mikroalg türlerinin biyofarmasötik üretiminde hammadde olarak kullanımı konusu tartışılmaktadır. .Bu fik - rin altında yatan temel neden mikro alglerin, biyolojik olarak aktif çok sayıda bileşenin üretimi için karbon dioksiti etkin bir şekilde kullanabilen hücre fabrikaları olmasıdır. Bununla birlikte, mikroalglerden rekombinant protein üretiminde kullanılması için çözülmesi gereken birkaç sorun mevcuttur. Kritik gereksinimlerden birisi salgılanan rekombinant protein - lerden biyo-uyumlu bir N-glikozilasyon profile üretmektir. Mikroalg türlerinin glikozilasyon makineleri ve N-glikan profilleri hakkındaki bilgiler oldukça sınırlıdır. Bu çalışmada, yeşil bir mikroalg türü olan Chlorella zofingiensis ‘in N-glikan profilinin karakterize edilmesi amaçlandı. Bu amaçla (gliko-)proteinleri içeren fotoototrofik olarak yetiştirilmiş Chlorella zofingiensis ekstreleri enzimatik olarak deglikozile edildi ve 2-aminobenzoik asit etiketi ile etiketlendi. Serbest hale getirilen N-glikanlar HILIC bazlı bir yaklaşımla saflaştırıldı ve MALDI-TOF (/TOF)-MS ile analiz edildi. Sonuçlar C. zofingiensis’in oligomannozidik türde N-glikan modellerini içerdiğini göstermiştir. Ek olarak, MALDI-MS tarafından C. zofingiensis’in N-glikosilasyon profilleri, oligomannosidik N-glikanların neredeyse yarısının fosforilenmiş olduğunu ortaya koymuştur.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | YAMAN M, Aladağ E, KAYILI H, KADIOĞLU Y, Salih B (2021). N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis. , 147 - 155. 10.15671/hjbc.717600 |
| Chicago | YAMAN Mehmet Emrah,Aladağ Erdinç,KAYILI Hacı Mehmet,KADIOĞLU Yücel,Salih Bekir N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis. (2021): 147 - 155. 10.15671/hjbc.717600 |
| MLA | YAMAN Mehmet Emrah,Aladağ Erdinç,KAYILI Hacı Mehmet,KADIOĞLU Yücel,Salih Bekir N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis. , 2021, ss.147 - 155. 10.15671/hjbc.717600 |
| AMA | YAMAN M,Aladağ E,KAYILI H,KADIOĞLU Y,Salih B N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis. . 2021; 147 - 155. 10.15671/hjbc.717600 |
| Vancouver | YAMAN M,Aladağ E,KAYILI H,KADIOĞLU Y,Salih B N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis. . 2021; 147 - 155. 10.15671/hjbc.717600 |
| IEEE | YAMAN M,Aladağ E,KAYILI H,KADIOĞLU Y,Salih B "N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis." , ss.147 - 155, 2021. 10.15671/hjbc.717600 |
| ISNAD | YAMAN, Mehmet Emrah vd. "N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis". (2021), 147-155. https://doi.org/10.15671/hjbc.717600 |
| APA | YAMAN M, Aladağ E, KAYILI H, KADIOĞLU Y, Salih B (2021). N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis. Hacettepe Journal of Biology and Chemistry, 49(2), 147 - 155. 10.15671/hjbc.717600 |
| Chicago | YAMAN Mehmet Emrah,Aladağ Erdinç,KAYILI Hacı Mehmet,KADIOĞLU Yücel,Salih Bekir N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis. Hacettepe Journal of Biology and Chemistry 49, no.2 (2021): 147 - 155. 10.15671/hjbc.717600 |
| MLA | YAMAN Mehmet Emrah,Aladağ Erdinç,KAYILI Hacı Mehmet,KADIOĞLU Yücel,Salih Bekir N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis. Hacettepe Journal of Biology and Chemistry, vol.49, no.2, 2021, ss.147 - 155. 10.15671/hjbc.717600 |
| AMA | YAMAN M,Aladağ E,KAYILI H,KADIOĞLU Y,Salih B N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis. Hacettepe Journal of Biology and Chemistry. 2021; 49(2): 147 - 155. 10.15671/hjbc.717600 |
| Vancouver | YAMAN M,Aladağ E,KAYILI H,KADIOĞLU Y,Salih B N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis. Hacettepe Journal of Biology and Chemistry. 2021; 49(2): 147 - 155. 10.15671/hjbc.717600 |
| IEEE | YAMAN M,Aladağ E,KAYILI H,KADIOĞLU Y,Salih B "N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis." Hacettepe Journal of Biology and Chemistry, 49, ss.147 - 155, 2021. 10.15671/hjbc.717600 |
| ISNAD | YAMAN, Mehmet Emrah vd. "N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis". Hacettepe Journal of Biology and Chemistry 49/2 (2021), 147-155. https://doi.org/10.15671/hjbc.717600 |
