Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients

Kızıltunç, Ahmet; ÖZKAN, HALİL İBRAHİM; Kiziltunc Ozmen, Hilal; Akgül, Hayati Murat; Açıkgöz, Gözde; Akgül, Nilgün

doi:10.3906/sag-2006-84

Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients

Gözde DERİNDAĞ, (Pamukkale Üniversitesi, Diş Hekimliği Fakültesi, Ağız, Diş ve Çene Radyolojisi Anabilim Dalı, Denizli, Türkiye)

Hayati Murat AKGÜL, (Pamukkale Üniversitesi, Diş Hekimliği Fakültesi, Ağız, Diş ve Çene Radyolojisi Anabilim Dalı, Denizli, Türkiye)

Ahmet KIZILTUNÇ, (Atatürk Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Erzurum, Türkiye)

Halil İbrahim ÖZKAN, (Atatürk Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Erzurum, Türkiye)

Hilal KIZILTUNÇ ÖZMEN, (Atatürk Üniversitesi, Tıp Fakültesi, Radyasyon Onkolojisi Anabilim Dalı, Erzurum, Türkiye)

Nilgün AKGÜL (Pamukkale Üniversitesi, Diş Hekimliği Fakültesi, Restoratif Diş Hekimliği Anabilim Dalı, Denizli, Türkiye)

Turkish Journal of Medical Sciences

1 0

Yıl: 2021 Cilt: 51 Sayı: 2 Sayfa Aralığı: 644 - 649 Metin Dili: İngilizce DOI: 10.3906/sag-2006-84 İndeks Tarihi: 21-01-2022

Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients

Öz:

Background/aim: It is believed that radiotherapy has important effects on oxidant/antioxidant systems. Oxidative stress occurs when the balance between oxidant formation and antioxidant defense is disrupted in favor of oxidants. The aim of this study was to determine the biochemical changes in saliva pre- and postradiotherapy in head-neck radiotherapy patients and to find out the effects of radiation on glutathione (GSH), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels in saliva. Materials and methods: This study included 16 patients undergoing head-neck radiotherapy in Atatürk University Research Hospital. The levels of GSH, GSH-Px, and MDA were measured in saliva samples taken from the patients pre- and postradiotherapy. The same biochemical parameters were also measured in saliva samples from 30 healthy individuals who did not undergo head-neck radiotherapy. The data obtained were analyzed using the paired t-test and the Mann–Whitney U test. Results: When the levels of GSH (P > 0.05), GSH-Px (P > 0.05), and MDA (P < 0.05) in saliva were compared pre- and postradiotherapy in the patient group, the only significant increase was detected in the MDA level postradiotherapy. When the pre- and postradiotherapy levels of saliva GSH (P < 0.01, P < 0.001, respectively), GSH-Px (P > 0.05, P < 0.05, respectively), and MDA (P < 0.01, P < 0.001, respectively) were compared with those of the control group, it was revealed that the GSH level was significantly lower and the MDA level was significantly higher in both pre- and postradiotherapy compared to the control group. Also, only the postradiotherapy saliva GSH-Px level was found to be significantly lower than the control group. Conclusion: These findings show that the changes in saliva GSH, GSH-Px, and MDA levels in patients with head-neck malignity intensified due to radiation.Key words: Glutathione, glutathione peroxidase, head and neck neoplasms, malondialdehyde, radiotherapy, saliva

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Çakur B, Miloğlu Ö, Harorlı A. Oral health care in patients underwent chemotherapy and radiotherapy. The Journal of Faculty of Dentistry of Atatürk University 2006; 2006 (3): 50- 55 (in Turkish).
2. Dirix P, Nuyts S, Van den Bogaert W. Radiation-induced xerostomia in patients with head and neck cancer: a literature review. Cancer 2006; 107(11): 2525-2534. doi: 10.1002/ cncr.22302
3. Imanimoghaddam M, Rahrooh M, Tafakhori Z, Zahedanaraki S, Homaeieshandiz F. Changes of parotid and submandibular glands caused by radiotherapy--an ultrasound evaluation. Dentomaxillofacial Radiology 2012; 41 (5): 379-384. doi: 10.1259/dmfr/17113005
4. Canakci CF, Cicek Y, Yildirim A, Sezer U, Canakci V. Increased levels of 8-hydroxydeoxyguanosine and malondialdehyde and its relationship with antioxidant enzymes in saliva of periodontitis patients. European Journal of Dentistry 2009; 3 (2): 100-106.
5. Halimi M, Parsian H, Asghari SM, Sariri R, Moslemi D et al. Clinical translation of human microRNA 21 as a potential biomarker for exposure to ionizing radiation. Translational Research: The Journal of Laboratory and Clinical Medicine 2014; 163 (6): 578-584. doi: 10.1016/j.trsl.2014.01.009
6. Khalil Arjmandi M, Moslemi D, Sadati Zarrini A, Ebrahimnezhad Gorji M, Mosapour A et al. Pre and post radiotherapy serum oxidant/antioxidant status in breast cancer patients: impact of age, BMI and clinical stage of the disease. Reports of Practical Oncology and Radiotherapy 2016; 21 (3): 141-148. doi: 10.1016/j.rpor.2015.12.009
7. Nguyen TT, Ngo LQ, Promsudthi A, Surarit R. Salivary lipid peroxidation in patients with generalized chronic periodontitis and acute coronary syndrome. Journal of Periodontology 2016; 87 (2): 134-141. doi: 10.1902/jop.2015.150353
8. Shariff AK, Patil SR, Shukla PS, Sontakke AV, Hendre AS et al. Effect of oral antioxidant supplementation on lipid peroxidation during radiotherapy in head and neck malignancies. Indian Journal of Clinical Biochemistry 2009; 24 (3): 307-311. doi: 10.1007/s12291-009-0057-3
9. Demirci S, Ozsaran Z, Celik HA, Aras AB, Aydin HH. The interaction between antioxidant status and cervical cancer: a case control study. Tumori 2011; 97 (3): 290-295. doi: 10.1700/912.10024
10. Gupta S, Singh KK, Vyas VJ, Chaturvedi VN, Reddy MV et al. Assessment of oxidative stress and effect of antioxidant supplementation during radiotherapy in carcinoma of upper digestive tract. Indian Journal of Clinical Biochemistry 2000; 15 (1): 52-55. doi: 10.1007/BF02873549
11. Khademi H, Khozeimeh F, Tavangar A, Amini S, Ghalayani P. The serum and salivary level of malondialdehyde, vitamins A, E, and C in patient with recurrent aphthous stomatitis. Advanced Biomedical Research 2014; 3: 246. doi: 10.4103/2277-9175.146366
12. Ziaudeen S, Ravindran R. Assessment of oxidant-antioxidant status and stress factor in recurrent aphthous stomatitis patients: case control study. Journal of Clinical and Diagnostic Research 2017; 11 (3): 01-04. doi: 10.7860/JCDR/2017/22894.9348
13. Fu TY, Tsai MH, Wang JS, Ger LP. Antioxidant enzymes in oral verrucous carcinoma. Journal of Oral Pathology and Medicine 2017; 46 (1): 46-49. doi: 10.1111/jop.12460
14. Metgud R, Bajaj S. Evaluation of salivary and serum lipid peroxidation, and glutathione in oral leukoplakia and oral squamous cell carcinoma. Journal of Oral Science 2014; 56 (2): 135-142. doi: 10.2334/josnusd.56.135
15. Babiuch K, Bednarczyk A, Gawlik K, Pawlica-Gosiewska D, Kesek B et al. Evaluation of enzymatic and non-enzymatic antioxidant status and biomarkers of oxidative stress in saliva of patients with oral squamous cell carcinoma and oral leukoplakia: a pilot study. Acta Odontologica Scandinavica 2019; 77 (6): 408- 418. doi: 10.1080/00016357.2019.1578409
16 .Shetty SR, Babu S, Kumari S, Shetty P, Hegde S et al. Status of salivary lipid peroxidation in oral cancer and precancer. Indian Journal of Medical and Paediatric Oncology 2014; 35 (2): 156- 158. doi: 10.4103/0971-5851.138990
17. Büyükköprü D, Dural S. The investigation of influence of head and neck radiotherapy on flow rate and Ph of saliva. Journal of Hacettepe University Faculty of Dentistry 2008; 32 (3): 71-78 (in Turkish).
18. Lin CY, Ju SS, Chia JS, Chang CH, Chang CW et al. Effects of radiotherapy on salivary gland function in patients with head and neck cancers. Journal of Dental Sciences 2015; 10 (3): 253- 262. doi: 10.1016/j.jds.2015.01.004
19. Khoubnasabjafari M, Ansarin K, Jouyban A. Salivary malondialdehyde as an oxidative stress biomarker in oral and systemic diseases. Journal of Dental Research, Dental Clinics, Dental Prospects 2016; 10 (2): 71-74. doi: 10.15171/ joddd.2016.011
20. Rodriguez de Sotillo D, Velly AM, Hadley M, Fricton JR. Evidence of oxidative stress in temporomandibular disorders: a pilot study. Journal of Oral Rehabilitation 2011; 38 (10): 722- 728. doi: 10.1111/j.1365-2842.2011.02216.x
21. Abdolsamadi H, Goodarzi MT, Ahmadi Motemayel F, Jazaeri M, Feradmal J et al. Reduction of melatonin level in patients with type II diabetes and periodontal diseases. Journal of Dental Research, Dental Clinics, Dental Prospects 2014; 8 (3): 160-165.doi: 10.5681/joddd.2014.029
22. Smriti K, Pai KM, Ravindranath V, Pentapati KC. Role of salivary malondialdehyde in assessment of oxidative stress among diabetics. Journal of Oral Biology and Craniofacial Research 2016; 6 (1): 41-44. doi: 10.1016/j.jobcr.2015.12.004
23. Abdolsamadi H, Rafieian N, Goodarzi MT, Feradmal J, Davoodi P et al. Levels of salivary antioxidant vitamins and lipid peroxidation in patients with oral lichen planus and healthy individuals. Chonnam Medical Journal 2014; 50 (2): 58-62. doi: 10.4068/cmj.2014.50.2.58
24. Shirzad A, Pouramir M, Seyedmajidi M, Jenabian N, Bijani A et al. Salivary total antioxidant capacity and lipid peroxidation in patients with erosive oral lichen planus. Journal of Dental Research, Dental Clinics, Dental Prospects 2014; 8 (1): 35-39. doi: 10.5681/joddd.2014.006
25. Babaee N, Hosseinkazemi H, Pouramir M, Khakbaz Baboli O, Salehi M et al. Salivary oxidant/antioxidant status and hematological parameters in patients with recurrent aphthous stomatitis. Caspian Journal of Internal Medicine 2016; 7 (1): 13-18.
26. Momen-Beitollahi J, Mansourian A, Momen-Heravi F, Amanlou M, Obradov S et al. Assessment of salivary and serum antioxidant status in patients with recurrent aphthous stomatitis. Medicina Oral, Patología Oral y Cirugía Bucal 2010; 15 (4): 557-561. doi: 10.4317/medoral.15.e557
27. Ahmadi-Motamayel F, Goodarzi MT, Jamshidi Z, Kebriaei R. Evaluation of salivary and serum antioxidant and oxidative stress statuses in patients with chronic periodontitis: acasecontrol study. Frontiers in Physiology 2017; 8: 189. doi: 10.3389/fphys.2017.00189
28. Balaji TM, Vasanthi HR, Rao SR. Gingival, plasma and salivary levels of melatonin in periodontally healthy individuals and chronic periodontitis patients: a pilot study. Journal of Clinical and Diagnostic Research 2015; 9 (3): 23-25. doi: 10.7860/ JCDR/2015/11311.5652
29. Dalai C, Ignat-Romanul I, Rosca E, Muresan M, Micle O et al. Correlation between histopathological aspects of periodontitis and biochemical changes of oxidative stress. Romanian Journal of Morphology and Embryology 2013; 54 (Suppl. 3): 817-822.
30. Lodhi K, Saimbi CS, Khan MA, Nath C, Shukla R. Evaluation of melatonin levels in saliva in gingivitis and periodontitis cases: a pilot study. Contemporary Clinical Dentistry 2016; 7 (4): 519-523. doi: 10.4103/0976-237X.194115
31. Almadori G, Bussu F, Galli J, Limongelli A, Persichilli S et al. Salivary glutathione and uric acid levels in patients with head and neck squamous cell carcinoma. Head & Neck 2007; 29 (7): 648-664. doi: 10.1002/hed.20579
32. Kaur J, Politis C, Jacobs R. Salivary 8-hydroxy-2- deoxyguanosine, malondialdehyde, vitamin C, and vitamin E in oral pre-cancer and cancer: diagnostic value and free radical mechanism of action. Clinical Oral Investigations 2016; 20 (2): 315-319. doi: 10.1007/s00784-015-1506-4
33. Yakob M, Fuentes L, Wang MB, Abemayor E, Wong DT. Salivary biomarkers for detection of oral squamous cell carcinoma - current state and recent advances. Current Oral Health Reports 2014; 1 (2): 133-141. doi: 10.1007/s40496-014- 0014-y
34. Frijhoff J, Winyard PG, Zarkovic N, Davies SS, Stocker R et al. Clinical relevance of biomarkers of oxidative stress. Antioxidants & Redox Signaling 2015; 23 (14): 1144-1170. doi: 10.1089/ars.2015.6317
35. Sowa P, Misiolek M, Pasinski B, Bartosz G, Soszynski M et al. Oxidative stress markers patients with parotid gland tumors: apilot study. BioMed Research International 2018; 2018: 4340871. doi: 10.1155/2018/4340871
36. Martinez-Useros J, Li W, Cabeza-Morales M, Garcia-Foncillas J. Oxidative stress: anew target for pancreatic cancer prognosis and treatment. Journal of Clinical Medicine 2017; 6 (3): 29. doi: 10.3390/jcm6030029
37. Bahar G, Feinmesser R, Shpitzer T, Popovtzer A, Nagler RM. Salivary analysis in oral cancer patients: DNA and protein oxidation, reactive nitrogen species, and antioxidant profile. Cancer 2007; 109 (1): 54-59. doi: 10.1002/cncr.22386
38. Choudhari SK, Chaudhary M, Gadbail AR, Sharma A, Tekade S. Oxidative and antioxidative mechanisms in oral cancer and precancer: a review. Oral Oncology 2014; 50 (1): 10-18. doi: 10.1016/j.oraloncology.2013.09.011
39. Agha-Hosseini F, Mirzaii-Dizgah I, Mikaili S, Abdollahi M. Increased salivary lipid peroxidation in human subjects with oral lichen planus. International Journal of Dental Hygiene 2009; 7 (4): 246-250. doi: 10.1111/j.1601-5037.2009.00365.x
40. Bansal A, Simon MC. Glutathione metabolism in cancer progression and treatment resistance. The Journal of Cell Biology 2018; 217 (7): 2291-2298. doi: 10.1083/jcb.201804161

APA	Açıkgöz G, Akgül H, Kızıltunç A, ÖZKAN H, Kiziltunc Ozmen H, Akgül N (2021). Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients. , 644 - 649. 10.3906/sag-2006-84
Chicago	Açıkgöz Gözde,Akgül Hayati Murat,Kızıltunç Ahmet,ÖZKAN HALİL İBRAHİM,Kiziltunc Ozmen Hilal,Akgül Nilgün Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients. (2021): 644 - 649. 10.3906/sag-2006-84
MLA	Açıkgöz Gözde,Akgül Hayati Murat,Kızıltunç Ahmet,ÖZKAN HALİL İBRAHİM,Kiziltunc Ozmen Hilal,Akgül Nilgün Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients. , 2021, ss.644 - 649. 10.3906/sag-2006-84
AMA	Açıkgöz G,Akgül H,Kızıltunç A,ÖZKAN H,Kiziltunc Ozmen H,Akgül N Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients. . 2021; 644 - 649. 10.3906/sag-2006-84
Vancouver	Açıkgöz G,Akgül H,Kızıltunç A,ÖZKAN H,Kiziltunc Ozmen H,Akgül N Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients. . 2021; 644 - 649. 10.3906/sag-2006-84
IEEE	Açıkgöz G,Akgül H,Kızıltunç A,ÖZKAN H,Kiziltunc Ozmen H,Akgül N "Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients." , ss.644 - 649, 2021. 10.3906/sag-2006-84
ISNAD	Açıkgöz, Gözde vd. "Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients". (2021), 644-649. https://doi.org/10.3906/sag-2006-84

APA	Açıkgöz G, Akgül H, Kızıltunç A, ÖZKAN H, Kiziltunc Ozmen H, Akgül N (2021). Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients. Turkish Journal of Medical Sciences, 51(2), 644 - 649. 10.3906/sag-2006-84
Chicago	Açıkgöz Gözde,Akgül Hayati Murat,Kızıltunç Ahmet,ÖZKAN HALİL İBRAHİM,Kiziltunc Ozmen Hilal,Akgül Nilgün Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients. Turkish Journal of Medical Sciences 51, no.2 (2021): 644 - 649. 10.3906/sag-2006-84
MLA	Açıkgöz Gözde,Akgül Hayati Murat,Kızıltunç Ahmet,ÖZKAN HALİL İBRAHİM,Kiziltunc Ozmen Hilal,Akgül Nilgün Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients. Turkish Journal of Medical Sciences, vol.51, no.2, 2021, ss.644 - 649. 10.3906/sag-2006-84
AMA	Açıkgöz G,Akgül H,Kızıltunç A,ÖZKAN H,Kiziltunc Ozmen H,Akgül N Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients. Turkish Journal of Medical Sciences. 2021; 51(2): 644 - 649. 10.3906/sag-2006-84
Vancouver	Açıkgöz G,Akgül H,Kızıltunç A,ÖZKAN H,Kiziltunc Ozmen H,Akgül N Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients. Turkish Journal of Medical Sciences. 2021; 51(2): 644 - 649. 10.3906/sag-2006-84
IEEE	Açıkgöz G,Akgül H,Kızıltunç A,ÖZKAN H,Kiziltunc Ozmen H,Akgül N "Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients." Turkish Journal of Medical Sciences, 51, ss.644 - 649, 2021. 10.3906/sag-2006-84
ISNAD	Açıkgöz, Gözde vd. "Evaluation of saliva glutathione, glutathione peroxidase, and malondialdehyde levels in head-neck radiotherapy patients". Turkish Journal of Medical Sciences 51/2 (2021), 644-649. https://doi.org/10.3906/sag-2006-84