The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats
Yıl: 2021 Cilt: 27 Sayı: 5 Sayfa Aralığı: 559 - 566 Metin Dili: İngilizce DOI: 10.9775/kvfd.2021.25390 İndeks Tarihi: 23-01-2022
The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats
Öz: This study examined the effect of Tarantula cubensis extract (TCE) on gentamicin-induced acute kidney injury in ovariectomized rats. A total of 40 female Wistar albino rats were randomly divided equally into five groups: Control (C), ovariectomy (O), Tarantula cubensis (OT), gentamicin (OG), and gentamicin + Tarantula cubensis (OGT). All rats except those in the C were ovariectomized. Kidney damage was created with gentamicin for OG and OGT. The OT and OGT were treated with a single dose of TCE. Blood, sera, and kidney tissue were taken at necropsy for evaluation. Total leukocyte count was higher in the OT compared to the others (P=0.002). Significant increases were also determined in serum urea, creatinine, aspartate aminotransferase, and total protein levels in the OT, OG, and OGT compared to the C and O. The glutathione level was low in the serum and kidney tissue of the OG, and the malondialdehyde level was high compared to the others (P<0.05). As a result of the use of TCE in gentamicin-induced acute kidney injury in ovariectomized rats, serum creatinine, urea, and malondialdehyde levels decreased in the OGT compared to the OG, the glutathione level increased, and the severity of histopathological findings decreased to milder levels. As a result; single dose of TCE partially reduced kidney damage in rats with gentamicin-induced acute kidney injury
Anahtar Kelime: Ovariektomili Ratlarda Gentamisin İle İndüklenen Akut Böbrek Hasarına Tarantula cubensis Ekstraktının Etkisi
Öz: Bu çalışmada ovariektomili ratlarda gentamisinle indüklenen akut böbrek hasarına Tarantula cubensis ekstraktının (TCE) etkisinin araştırılması amaçlandı. Çalışmada Wistar albino, dişi, yetişkin, 40 rat kullanıldı. Randomize şekilde her grupta 8 rat olacak şekilde 5 gruba ayrıldı. Gruplar; Kontrol grubu (K), ovariektomi grubu (O), Tarantula cubensis grubu (OT), gentamisin grubu (OG), gentamisin + Tarantula cubensis grubu (OGT) olarak belirlendi. K dışındaki tüm ratlara overektomi operasyonu yapıldı. OG ve OGT’de gentamisin ile böbrek hasarı oluşturuldu. OT ve OGT’de tek doz TCE ile tedavi uygulandı. Değerlendirme için ötenazi sonrası kan, serum ve böbrek dokusu alındı. Total lökosit sayısı OT’de diğer gruplara oranla yüksek bulundu (P=0.002). Serum biyokimyasal parametrelerde, K ve O’ya kıyasla OT, OG ve OGT’de serum üre, kreatinin, aspartat aminotransferaz ve total protein seviyelerinde önemli artışlar belirlendi. OG’de serum ve böbrek dokusunda glutatyon seviyesi düşük, malondialdehit seviyesi diğer gruplara kıyasla yüksek bulundu (P<0.05). Ovariektomili ratlarda gentamisinle indüklenen akut böbrek hasarında TCE kullanımı sonucunda, OGT’de, OG’ye kıyasla serum kreatinin, üre ve malondialdehit seviyesinin düştüğü, glutatyon seviyesinin yükseldiği ve histopatolojik bulguların şiddetinin daha hafif düzeylere indiği görüldü. Sonuç olarak gentamisinle indüklenen akut böbrek hasarında TCE tek doz uygulanması böbrekteki hasarı kısmen azalttı.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Yazar E: Kemoterapötikler. In, Yazar E, Traş B, Elmas M (Eds): Veteriner İlaç Rehberi Tedavi El Kitabı. 2. Baskı, 102-103, Atlas Yayınevi, Konya, 2018.
- 2. Shahrbaf FG, Assadi F: Drug-induced renal disorders. J Renal Inj Prev, 4 (3): 57-60, 2015. DOI: 10.12861/jrip.2015.12
- 3. Paueksakon P, Fogo AB: Drug-induced nephropathies. Histopathology, 70 (1): 94-108, 2017. DOI: 10.1111/his.13064
- 4. Sales GTM, Foresto RD: Drug-induced nephrotoxicity. Rev Assoc Med Bras, 66 (Suppl. 1): 82-90, 2020. DOI: 10.1590/1806-9282.66.s1.82
- 5. Walker PD, Shah SV: Evidence suggesting a role for hydroxyl radical in gentamicin-induced acute renal failure in rats. J Clin Invest, 81 (2): 334- 341, 1988. DOI: 10.1172/JCI113325
- 6. Randjelović P, Veljković S, Stojiljković N, Sokolović D, Ilić I: Gentamicin nephrotoxicity in animals: Current knowledge and future perspectives. EXCLI J, 16, 388-399, 2017. DOI: 10.17179/excli2017-165
- 7. Udupa V, Prakash V: Gentamicin induced acute renal damage and its evaluation using urinary biomarkers in rats. Toxicol Rep, 6, 91-99, 2019. DOI: 10.1016/j.toxrep.2018.11.015
- 8. Savin VJ, Beason-Griffin C, Richardson WP: Ultrafiltration coefficient of isolated glomeruli of rats aged 4 days to maturation. Kidney Int, 28 (6): 926-931 1985. DOI: 10.1038/ki.1985.219
- 9. Yanagida C, Ito K, Komiya I, Horie T: Protective effect of fosfomycin on gentamicin-induced lipid peroxidation of rat renal tissue. Chem Biol Interact, 148 (3): 139-147, 2004. DOI: 10.1016/j.cbi.2004.05.005
- 10. Aygün FÖ, Akçam FZ, Kaya O: Sıçanlarda deneysel gentamisin nefrotoksisitesinde oksidatif stresin rolünün ve olası oksidatif stres üzerine kafeik asit Fenetil Ester’in etkisinin araştırılması. Yüksek Lisans Tezi, Süleyman Demirel Üniv. Tıp Fak., 2010.
- 11. Aslan S, Fındık M, Kalender H, Celebi M, Izgur H, Handler J: Improvement of postpartal fertility of cows by Pulsatilla miniplex®. Wien Tierärztl Msch, 87 (12): 359-362, 2000.
- 12. Kaçar C, Zonturlu AK, Oral H, Yıldız S, Arı UC: İneklerde eken puerperal dönemde Theranekron® uygulamalarinin uterus involusyonu ve vaginal akinti üzerine etkisi. Kafkas Univ Vet Fak Derg, 13 (1): 11-15, 2007. DOI: 10.9775/kvfd.2006.36-A
- 13. Coşkun D: Veteriner Destek Tedavi: Tarantula cubensis alkolik ekstraktı, inaktif Parapoxvirüs ovis ve Corynebacterium cutis lizatı. Dicle Univ Vet Fak Derg, 10 (1): 30-37, 2017.
- 14. Albay MK, Sahinduran S, Kale M, Karakurum MC, Sezer K: Influence of Tarantula cubensis extract on the treatment of the oral lesions in cattle with bluetongue disease. Kafkas Univ Vet Fak Derg, 16 (4): 593-596, 2010. DOI: 10.9775/kvfd.2009.1192
- 15. Corum O, Er A, Dik B: Investigation of the effect of Tarantula cubensis extract on acute phase response. Acta Sci Vet, 44:1414, 2016. DOI: 10.22456/1679-9216.81289
- 16. Dik B, Er A, Çorum O: Effect of alcoholic extract of Tarantula cubensis (Theranekron®) on serum thiobarbituric acid-reactive species concentrations in sheep. Eurasian J Vet Sci, 30 (2): 68-71, 2014. DOI: 10.15312/EurasianJVetSci.201425921
- 17. Eren C, Akşit D: Deneysel gentamisin nefrotoksisitesi oluşturulan ratlarda Tarantula cubensis ekstraktının koruyucu etkilerinin araştırılması. Yüksek Lisans Tezi, Balıkesir Üniv. Sağlık Bil. Enst., 2020.
- 18. Parlakpınar H, Örüm MH, Acet A: İlaca bağlı nefrotoksisitede serbest oksijen radikalleri. F Ü Sağ Bil Tıp Derg, 27 (1): 51-56, 2013.
- 19. Gluhovschi G, Gluhovschi A, Anastasiu D, Petrica L, Gluhovschi C, Velciov S: Chronic kidney disease and the involvement of estrogen hormones in its pathogenesis and progression. Rom J Intern Med, 50 (2):135-144, 2012.
- 20. Robert R, Ghazali DA, Favreau F, Mauco G, Hauet T, Goujon JM: Gender difference and sex hormone production in rodent renal ischemia reperfusion injury and repair. J Inflamm, 8:14, 2011. DOI: 10.1186/1476- 9255-8-14
- 21. Müller V, Losonczy G, Heemann U, Vannay A, Fekete A, Reusz G, Tulassay T, Szabó AJ: Sexual dimorphism in renal ischemia-reperfusion injury in rats: Possible role of endothelin. Kidney Int, 62 (4):1364-1371, 2002. DOI: 10.1111/j.1523-1755.2002.kid590.x
- 22. Makav M, Eroğlu HA: Rotenon ile deneysel olarak oluşturulmuş parkinson modelinde östrojenin etkisi. Doktora Tezi, Kafkas Üniv. Sağlık Bil. Enst., 2018.
- 23. Silan C, Uzun Ö, Çomunoğlu NÜ, Gokçen S, Bedirhan S, Cengiz M: Gentamicin-induced nephrotoxicity in rats ameliorated and healing effects of resveratrol. Biol Pharm Bull, 30 (1): 79-83, 2007. DOI: 10.1248/ bpb.30.79
- 24. Yoshioka T, Kawada K, Shimada T, Mori M: Lipid peroxidation in maternal and cord blood and protective mechanism against activatedoxygen toxicity in the blood. Am J Obstet Gynecol, 135 (3): 372-376, 1979. DOI: 10.1016/0002-9378(79)90708-7
- 25. Beutler E, Duron O, Kelly B: Improved method for the determination of blood glutathione. J Lab Clin Med, 61, 882-888, 1963.
- 26. Bacus S, Flowers JL, Press MF, Bacus LW, McCarty Jr KS: The evaluation of estrogen receptor in primary breast carcinoma by computerassisted image analysis. Am J Clin Pathol, 90 (3): 233-239, 1988. DOI: 10.1093/ajcp/90.3.233
- 27. Etim NN, Williams ME, Akpabio U, Offiong EEA: Haematological parameters and factors affecting their values. Agric Sci, 2 (1): 37-47, 2014.
- 28. Durmuş I, Ince S, Salim MN, Eryavuz A, Küçükkurt I: Gentamisin verilen sıçanlara bor uygulamasının hematolojik parametre düzeylerine etkileri. Kocatepe Vet J, 11 (2): 140-147, 2018. DOI: 10.30607/kvj.394370
- 29. Lopez-Novoa JM, Quiros Y, Vicente L, Morales AI, LopezHernandez FJ: New insights into the mechanism of aminoglycoside nephrotoxicity: An integrative point of view. Kidney Int, 79 (1): 33-45, 2011. DOI: 10.1038/ki.2010.337
- 30. Horibe T, Matsui H, Tanaka M, Nagai H, Yamaguchi Y, Kato K, Kikuchi M: Gentamicin binds to the lectin site of calreticulin and inhibits its chaperone activity. Biochem Biophys Res Commun, 323 (1): 281-287, 2004. DOI: 10.1016/j.bbrc.2004.08.099
- 31. Zhang J, Zou Y, Cheng-Jing Y, Xiang-Heng L, Wang XP, Yu XJ, Wang J: Pioglitazone alleviates cisplatin nephrotoxicity by suppressing mitochondria‐mediated apoptosis via SIRT1/p53 signalling. J Cell Mol Med, 24 (20): 11718-11728, 2020. DOI: 10.1111/jcmm.15782
- 32. Reiter RJ, Tan D, Sainz RM, Mayo JC, Burillo SL: Melatonin: Reducing the toxicity and increasing the efficacy of drugs. J Pharm Pharmacol, 54 (10): 1299-1321, 2002. DOI: 10.1211/002235702760345374
- 33. Gülmez A, Çeliker H, Biçim S, Aydın S, Kuloğlu T: The investigation of the therapeutic effect of adropin on gentamicin-induced experimental nephropathy. Turk Neph Dial Transpl, 27 (3): 240-248, 2018. DOI: 10.5262/ tndt.2018.3121
- 34. Karahan İ, Ateşşahin, A, Yılmaz S, Çeribaşı AO, Sakin F: Protective effect of lycopene on gentamicin-induced oxidative stress and nephrotoxicity in rats. Toxicology, 215 (3): 198-204, 2005. DOI: 10.1016/j.tox. 2005.07.007
- 35. Çenesiz S: The role of oxidant and antioxidant parameters in the infectious diseases: A systematic literature review. Kafkas Univ Vet Fak Derg, 26 (6): 849-858, 2020. DOI: 10.9775/kvfd.2020.24618
- 36. Morales AI, Detaille D, Prieto M, Puente A, Briones E, Arévalo M, Leverve X, Novoa JML, El-Mir MY: Metformin prevents experimental gentamicin-induced nephropathy by a mitochondria-dependent pathway. Kidney Int, 77 (10): 861-869, 2010. DOI: 10.1038/ki.2010.11
- 37. Mohamed DI, Khairy E, Saad SS, Habib EK, Hamouda MA: Potential protective effects of dapagliflozin in gentamicin induced nephrotoxicity rat model via modulation of apoptosis associated miRNAs. Gene, 707, 198-204, 2019. DOI: 10.1016/j.gene.2019.05.009
- 38. Galdino PM, Alexandre LN, Pacheco LF, Junior RDSL, de Paula JR, Pedrino GR, Xavier CH, Ferreira PM: Nephroprotective effect of Rudgea viburnoides (Cham.) Benth leaves on gentamicin-induced nephrotoxicity in rats. J Ethnopharmacol, 201, 100-107, 2017. DOI: 10.1016/j.jep. 2017.02.035
- 39. El-Khaiat HM, Ghanem MM, Fayed HM, El-Raof YMA, El-Attar HM: Influence of vit. C on Egyptian Baladi goats (Capra hircus) with gentamicin induced nephrotoxicit. BJAS, 2 (2): 91-98, 2017. DOI: 10.21608/ bjas.2017.165725
| APA | AKYÜZ E, MAKAV M, KURU M, Bayram P (2021). The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats. , 559 - 566. 10.9775/kvfd.2021.25390 |
| Chicago | AKYÜZ Enes,MAKAV MUSTAFA,KURU Mushap,Bayram Pınar The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats. (2021): 559 - 566. 10.9775/kvfd.2021.25390 |
| MLA | AKYÜZ Enes,MAKAV MUSTAFA,KURU Mushap,Bayram Pınar The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats. , 2021, ss.559 - 566. 10.9775/kvfd.2021.25390 |
| AMA | AKYÜZ E,MAKAV M,KURU M,Bayram P The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats. . 2021; 559 - 566. 10.9775/kvfd.2021.25390 |
| Vancouver | AKYÜZ E,MAKAV M,KURU M,Bayram P The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats. . 2021; 559 - 566. 10.9775/kvfd.2021.25390 |
| IEEE | AKYÜZ E,MAKAV M,KURU M,Bayram P "The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats." , ss.559 - 566, 2021. 10.9775/kvfd.2021.25390 |
| ISNAD | AKYÜZ, Enes vd. "The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats". (2021), 559-566. https://doi.org/10.9775/kvfd.2021.25390 |
| APA | AKYÜZ E, MAKAV M, KURU M, Bayram P (2021). The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27(5), 559 - 566. 10.9775/kvfd.2021.25390 |
| Chicago | AKYÜZ Enes,MAKAV MUSTAFA,KURU Mushap,Bayram Pınar The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27, no.5 (2021): 559 - 566. 10.9775/kvfd.2021.25390 |
| MLA | AKYÜZ Enes,MAKAV MUSTAFA,KURU Mushap,Bayram Pınar The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.27, no.5, 2021, ss.559 - 566. 10.9775/kvfd.2021.25390 |
| AMA | AKYÜZ E,MAKAV M,KURU M,Bayram P The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(5): 559 - 566. 10.9775/kvfd.2021.25390 |
| Vancouver | AKYÜZ E,MAKAV M,KURU M,Bayram P The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(5): 559 - 566. 10.9775/kvfd.2021.25390 |
| IEEE | AKYÜZ E,MAKAV M,KURU M,Bayram P "The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27, ss.559 - 566, 2021. 10.9775/kvfd.2021.25390 |
| ISNAD | AKYÜZ, Enes vd. "The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27/5 (2021), 559-566. https://doi.org/10.9775/kvfd.2021.25390 |
