TY  - JOUR
TI  - Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease
AB  - The aim of this study was to investigate Reactive Oxygen Species (ROS)-induced lipidperoxidation, protein and DNA damage with oxidative stress markers such as 4-hydroxy-2- nonenal (4-HNE), dityrosine (DT) and 8-hydroxy-2-deoxyguanosine (8-OHdG) in lambs withWhite Muscle Disease (WMD). The material of this study consisted of tissue samples taken from20 sheep with WMD and 6 healthy control groups brought to Pathology Department for routinehistopathological diagnosis between 2012 and 2019. In macroscopic examinations, chalky-whitelarge necrotic areas on the epicardial and especially on the endocardial surfaces of the right andleft ventricular walls were observed. In microscopic examinations, it was observed that thedegenerated muscle fibers in the heart were homogeneously pink in color, lost their cytoplasmiccitration, swelled and their nuclei were pyknotic. Calcifications in necrotic and degenerated areaswere detected. All white muscle disease cases were immune positive for 4-HNE, DT and 8-OHdGexpressions in heart tissues. A significant difference (P<0.05) was detected between the groups interms of immunoreactivity to 4-HNE, DT and 8-OHdG in immunohistochemical andimmunofluorescence staining findings. In conclusion, our study showed that formation of ROS thatis important in pathogenesis of WMD causes not only lipid peroxidation, but also proteinmodification and DNA damage.
AU  - KARATAŞ, Özhan
AU  - NUHOĞLU, HİLMİ
AU  - YILDIZ, Ayfer
AU  - BEYTUT, ENVER
AU  - DAĞ, SERPİL
AU  - Mendil, Ali Sefa
AU  - Karakurt, Emin
PY  - 2021
JO  - Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi
VL  - 35
IS  - 2
SN  - 1308-9323
SP  - 109
EP  - 113
DB  - TRDizin
UR  - http://search/yayin/detay/484769
ER  -